Low miR-1273a expression predicts poor prognosis of colon cancer and facilitates tumor cell proliferation, migration, and invasion

Sun, Lei; Zhou, Xin; Jiang, Qian; Zhuang, Yibo; Li, Dongzheng

doi:10.1590/1414-431x202010394

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Colon Cancer is a refractory disease with high morbidity and mortality worldwide. [16][17][18] Although surgical resection can improve the prognosis of some colon cancer patients, the prognosis of colon cancer with perineural invasion is still poor. [19][20][21] Therefore, exploring the biology and novel prognostic biomarkers of Colon Cancer with perineural invasion may provide clinicians new tools to treat the disease.…”

Section: Discussionmentioning

confidence: 99%

The Midkine-related ceRNA Network is a Prognostic Marker of Colon Cancer With Perineural Invasion

Wang¹,

Ye²,

Cheng³

et al. 2021

Preprint

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BackgroundPerineural invasion (PNI) is a prominent characteristic of multiple solid tumors and indicates poor prognosis[1, 2]. A growing body of evidence emphasizes the critical role of competitive endogenous RNA(ceRNA) regulatory networks in various human cancers. However, the complexity and behavior characteristics of the ceRNA network in colon cancer with perineural invasion were still unclear. In this study, we aimed to clarify a midkine(MDK)-related ceRNA regulatory network and identify potential prognostic markers associated with colon cancer with perineural invasion.Material and MethodsWe extract the expression profiles of three RNAs (long non-coding RNAs [lncRNAs], microRNAs [miRNAs], and mRNAs) from The Cancer Genome Atlas (TCGA) database. In order to construct the lncRNA-miRNA-mRNA triple regulatory network in colon cancer with perineural invasion, we performed joint analysis in the MDKhigh and MDKlow expression groups, as well as the colon cancer and paracancerous tissue groups. Kaplan-Meier method was used to plot the Overall survival (OS) curves, and the log-rank test was used for testing. Univariate and multivariate Cox regression analysis were used to determine OS-related characteristics.ResultsKCNQ1OT1-miR-454-3p/miR-204-5p-MAP1B ceRNA network related to the prognosis of colon cancer was obtained through bioinformatics analysis. Importantly, we determined the KCNQ1OT1/MAP1B axis in the ceRNA through correlation analysis, and through Cox regression analysis it seems to be a clinical prognostic model. ConclusionIn conclusion, the current study constructing a ceRNA based KCNQ1OT1/MAP1B axis may be a novel important prognostic factor for the diagnosis and prognosis of colon cancer with perineural invasion.

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Section: Discussionmentioning

confidence: 99%

The Midkine-related ceRNA Network is a Prognostic Marker of Colon Cancer With Perineural Invasion

Wang¹,

Ye²,

Cheng³

et al. 2021

Preprint

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“…In developing countries, around one quarter of patients with COAD are in an advanced stage at diagnosis, and thus have lost their opportunities for obtaining a radical surgery [3]. Even though the consensus policy in the diagnosis and treatment of COAD has developed rapidly in recent years, it is still an important issue because of its high prevalence and poor prognosis [4]. Therefore, novel prognostic biomarkers and therapeutic targets for COAD are urgently needed in order to overcome these problems.…”

Section: Introductionmentioning

confidence: 99%

Elevated expression of CXCL3 in colon cancer promotes malignant behaviors of tumor cells in an ERK-dependent manner

Cheng

Yang

Liang

et al. 2023

Preprint

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Background: CXCL3 is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. Recently, numerous evidence indicated that CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression. However, the expression profile of CXCL3 and the exact molecular mechanism behind the role of CXCL3 in colon adenocarcinoma (COAD) has not been fully elucidated. Methods: The expression and clinical significance of CXCL3 mRNA and protein in the tissues from COAD patients were estimated using bioinformatics and immunohistochemistry assays. The expression and roles of exogenous administration or overexpression of CXCL3 in HT-29 and SW480 COAD cells were determined using ELISA, CCK-8 and Transwell assays. Mechanically, CXCL3-induced malignant behaviors were elucidated using western blotting assay and ERk1/2 inhibitor PD98059. Results: TCGA-COAD data analysis revealed that CXCL3 mRNA is highly expressed and has high clinical diagnostic accuracy in COAD. Increased expression of CXCL3 mRNA was associated with patient’s clinical stage, race, gender, age, histological subtype, nodal mestastasis and TP53 mutation status. Similarly, immunohistochemistry assay also exhibited that CXCL3 protein in COAD tissues was significantly up-regulated. Gene expression associated assay implied that CXCL1 and CXCL2 were markedly correlated with CXCL3 in COAD. PPI analysis revealed that CCNB1, MAD2L1, H2AFZ and CXCL2 may be the important molecules involved in CXCL3-related tumor biology. GSEA analysis revealed that CXCL3 was mainly enriched in the cell cycle, DNA replication, NOD-like receptors, NOTCH and TGF-β Signal pathways. In vitro, exogenous administration or overexpression of CXCL3 resulted in increased malignant behaviors of HT-29 and SW480 cells, and overexpression of CXCL3 affected the expression of genes related to extracellular signal regulated kinase (ERK) pathway, including ERK1/2, p-ERK, Bcl-2, Bax and Cyclin D1. In addition, CXCL3-induced malignant behaviors in HT-29 and SW480 cells were obviously attenuated following treatment with ERK inhibitor PD98059. Conclusion: CXCL3 is upregulated in COAD and plays a crucial role in the control of malignant behaviors of tumor cells, which indicated its involvement in the pathogenesis of COAD.

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“…In developing countries, around one quarter of patients with COAD are in an advanced stage at diagnosis, and thus have lost their opportunities for obtaining a radical surgery [ 3 ]. Even though the consensus policy in the diagnosis and treatment of COAD has developed rapidly in recent years, it is still an important issue because of its high prevalence and poor prognosis [ 4 ]. Therefore, novel prognostic biomarkers and therapeutic targets for COAD are urgently needed in order to overcome these problems.…”

Section: Introductionmentioning

confidence: 99%

Elevated expression of CXCL3 in colon cancer promotes malignant behaviors of tumor cells in an ERK-dependent manner

Cheng,

Yang,

Liang

et al. 2023

BMC Cancer

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Background CXC chemokine ligand 3 (CXCL3) is a member of CXC-type chemokine family that is identified as a major regulator in immune and inflammation responses. Recently, numerous evidence indicated that CXCL3 is broadly expressed in various human tumor types, and it is also known to play a critical role in mediating tumor development and progression. However, the expression profile of CXCL3 and the exact molecular mechanism behind the role of CXCL3 in colon adenocarcinoma (COAD) has not been fully elucidated. Methods The expression and clinical significance of CXCL3 mRNA and protein in the tissues from COAD patients were estimated using bioinformatics and immunohistochemistry assays. The expression and roles of exogenous administration or overexpression of CXCL3 in HT-29 and SW480 COAD cells were determined using enzyme-linked immunosorbent assay(ELISA), Cell Counting Kit-8 (CCK-8) and Transwell assays. Mechanically, CXCL3-induced malignant behaviors were elucidated using western blotting assay and extracellular signal-regulated protein kinase 1/2 (ERk1/2) inhibitor PD98059. Results The cancer genome atlas (TCGA)-COAD data analysis revealed that CXCL3 mRNA is highly expressed and has high clinical diagnostic accuracy in COAD. Increased expression of CXCL3 mRNA was associated with patient’s clinical stage, race, gender, age, histological subtype, nodal mestastasis and tumor protein 53 (TP53) mutation status. Similarly, immunohistochemistry assay also exhibited that CXCL3 protein in COAD tissues was significantly up-regulated. Gene expression associated assay implied that CXC chemokine ligand 1 (CXCL1) and CXC chemokine ligand 2 (CXCL2) were markedly correlated with CXCL3 in COAD. Protein-protein interaction (PPI) analysis revealed that cyclin B1 (CCNB1), mitotic arrest deficient 2 like 1 (MAD2L1), H2A family member Z (H2AFZ) and CXCL2 may be the important protein molecules involved in CXCL3-related tumor biology. Gene set enrichment analysis (GSEA) analysis revealed that CXCL3 was mainly enriched in the cell cycle, DNA replication, NOD-like receptors, NOTCH and transforming growth factor-β (TGF-β) Signal pathways. In vitro, exogenous administration or overexpression of CXCL3 resulted in increased malignant behaviors of HT-29 and SW480 cells, and down-regulation of CXCL3 expression inhibited the malignant behaviors of these tumor cells. In addition, overexpression of CXCL3 affected the expression of genes related to extracellular signal regulated kinase (ERK) pathway, including ERK1/2, p-ERK, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and Cyclin D1. Finally, CXCL3-induced malignant behaviors in HT-29 and SW480 cells were obviously attenuated following treatment with ERK inhibitor PD98059. Conclusion CXCL3 is upregulated in COAD and plays a crucial role in the control of malignant behaviors of tumor cells, which indicated its involvement in the pathogenesis of COAD.

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Low miR-1273a expression predicts poor prognosis of colon cancer and facilitates tumor cell proliferation, migration, and invasion

Cited by 4 publications

References 32 publications

The Midkine-related ceRNA Network is a Prognostic Marker of Colon Cancer With Perineural Invasion

The Midkine-related ceRNA Network is a Prognostic Marker of Colon Cancer With Perineural Invasion

Elevated expression of CXCL3 in colon cancer promotes malignant behaviors of tumor cells in an ERK-dependent manner

Elevated expression of CXCL3 in colon cancer promotes malignant behaviors of tumor cells in an ERK-dependent manner

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