microRNA-130b downregulation potentiates chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SOX9

Zhang, Penggui; Gao, Guangming; Zhou, Ziyu; He, Xuejun

doi:10.1590/1414-431x202010345

Cited by 11 publications

(4 citation statements)

References 39 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR‐690 regulates the differentiation of induced pluripotent stem cells (IPSCs) into insulin‐producing cells by targeting Sox9 (Xu et al, 2019). miR‐130b enhances chondrogenic differentiation of BMSCs by targeting Sox9 (Zhang et al, 2021). rno‐miR‐90 is a new miRNA sequence discovered by our team, and this study preliminarily verified its ability to promote the directed differentiation of BMSCs into chondrocytes through Smoc2‐targeting 3′‐UTR.…”

Section: Discussionmentioning

confidence: 99%

rno‐miR‐90 promotes chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SPARC‐related modular calcium binding 2

Ouyang

Wang

Xie

et al. 2023

The Anatomical Record

View full text Add to dashboard Cite

Bone marrow mesenchymal stem cells (BMSCs) have the ability to differentiate into chondrocytes. In the differentiation of BMSCs into chondrocytes, micro-RNAs (miRNAs) play an important role. rno-miR-90 is a new miRNA discovered by our research team, and its role in chondrogenic differentiation of BMSCs is unknown. This study aimed to investigate whether rno-miR-90 could promote chondrogenic differentiation of BMSCs by regulating secreted protein acidic and rich in cysteine-related modular calcium binding 2 (Smoc2). First, BMSCs chondroblast differentiation was successfully induced in vitro by classical induction method of transforming growth factor (TGF)-β3. On this basis, we transfected rno-miR-90 mimic and inhibitor, and confirmed that rno-miR-90 mimic could promote the differentiation of BMSCs into chondrocytes by real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. In addition, we demonstrated that Smoc2 was a target gene of rno-miR-90 by dual-luciferase reporter assay, and confirmed that rno-miR-90 mimic could inhibit the expression of Smoc2 by RT-qPCR and western blotting. In order to further prove the targeting relationship between rno-miR-90 and Smoc2, we constructed three interfering fragments of Smoc2, and proved that silencing Smoc2 could promote the differentiation of BMSCs into chondrocytes at the transcriptional and protein levels. Finally, we constructed a carrier scaffold for ectopic chondrogenic differentiation in vivo, and confirmed that rno-miR-90 mimic and siSmoc2 could promote chondrogenic differentiation of BMSCs by Alcian blue staining and immunohistochemistry.

show abstract

Section: Discussionmentioning

confidence: 99%

rno‐miR‐90 promotes chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SPARC‐related modular calcium binding 2

Ouyang

Wang

Xie

et al. 2023

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…For example, miR-130b interacting with SOX9 inhibits BMSC chondrogenesis. 13 MiR-132-3p enhances MSC differentiation into chondrocytes by regulating ADAMTS-5. 14 MiR-410 targets Wnt3a to boost BMSC chondrogenesis.…”

Section: Introductionmentioning

confidence: 99%

MiR-362-5p inhibits cartilage repair in osteoarthritis via targeting plexin B1

Zhang

et al. 2022

J Orthop Surg (Hong Kong)

View full text Add to dashboard Cite

Background and objectives Chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) exerts great function during the pathogenesis of osteoarthritis (OA). Studies have reported the association of plexin B1 (PLXNB1) with OA pathogenesis. In this study, the upstream mechanism and function of PLXNB1 in this disease were explored. Methods Flow cytometry was applied to test BMSC characterization. Chondrogenic differentiation of BMSCs was evaluated by Alcian blue staining. The expression of PLXNB1, miR-362-5p, miR-501-5p, miR-1827, miR-500-5p was measured using RT-qPCR analysis. The protein levels of PLXNB1, Aggrecan, and Silent information regulator factor 2-related enzyme 1 (SIRT1) were determined by western blotting. Binding relationship between miR-362-5p and PLXNB1 was confirmed using bioinformatics analysis and luciferase reporter assay. The in vivo model of OA was established in Sprague-Dawley rats which received medial meniscus instability surgery. For histopathological examination, cartilage tissues in the knee joint of rats were stained with hematoxylin and eosin. Micro-CT analysis was employed to observe the changes of morphometric indices including average trabecular separation, average trabecular thickness, and bone volume fraction. Results BMSCs were identified to possess the characteristics of mesenchymal stem cells. PLXNB1 was observed to be highly expressed during chondrogenic differentiation of BMSCs and PLXNB1 overexpression promoted BMSC chondrogenic differentiation. Mechanically, PLXNB1 was targeted by miR-362-5p. In rescue assays, miR-362-5p reversed the effects of PLXNB1 on chondrogenic differentiation of BMSCs. In the in vivo experiments, upregulated PLXNB1 expression alleviated joint injury of OA rats. Additionally, overexpressed miR-362-5p and downregulated PLXNB1 expression levels were detected in OA rats. Conclusion MiR-362-5p promotes OA progression by suppressing PLXNB1.

show abstract

“…For instance, miR-590-5p facilitates chondrocyte apoptosis, proliferation, and inflammation in OA by targeting fibroblast growth factor 18 [ 15 ]. Depletion of miR-130b promotes chondrogenic differentiation of BMSCs in OA by targeting SRY-box transcription factor 9 [ 16 ]. Recently, miR-20a-5p has been indicated to be implicated in multiple cellular processes of disorders like osteogenic and adipogenic differentiation [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-20a-5p facilitates cartilage repair in osteoarthritis via suppressing mitogen-activated protein kinase kinase kinase 2

et al. 2022

View full text Add to dashboard Cite

Bone marrow mesenchymal stem cell (BMSC) chondrogenic differentiation contributes to the treatment of osteoarthritis (OA). Numerous studies have indicated that microRNAs (miRNAs) regulate the pathogenesis and development of multiple disorders, including OA. Nevertheless, the role of miR-20a-5p in OA remains obscure. Forty male C57BL/6 mice were divided into four groups and were surgically induced OA or underwent sham surgery in the presence or absence of miR-20a-5p. Flow cytometry was implemented to detect surface markers of BMSCs. Reverse transcription quantitative polymerase chain reaction revealed the upregulation of miR-20a-5p during BMSC chondrogenic differentiation. Western blotting displayed that miR-20a-5p inhibition decreased protein levels of cartilage matrix markers but enhanced those of catabolic and hypertrophic chondrocyte markers in BMSCs. Alcian blue staining, hematoxylin‑eosin staining and micro-CT revealed that miR-20a-5p inhibition restrained chondrogenic differentiation and miR-20a-5p overexpression promoted the repair of damaged cartilage and subchondral bone, respectively. Luciferase reporter assay identified that mitogen activated protein kinase kinase kinase 2 (Map3k2) was a target of miR-20a-5p in BMSCs. Moreover, the expression of miR-20a-5p and Map3k2 was negatively correlated in murine cartilage tissues. Knocking down Map3k2 could rescue the suppressive influence of miR-20a-5p inhibition on chondrogenic differentiation of BMSCs. In conclusion, miR-20a-5p facilitates BMSC chondrogenic differentiation and contributes to cartilage repair in OA by suppressing Map3k2.

show abstract

microRNA-130b downregulation potentiates chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SOX9

Cited by 11 publications

References 39 publications

rno‐miR‐90 promotes chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SPARC‐related modular calcium binding 2

rno‐miR‐90 promotes chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SPARC‐related modular calcium binding 2

MiR-362-5p inhibits cartilage repair in osteoarthritis via targeting plexin B1

MiR-20a-5p facilitates cartilage repair in osteoarthritis via suppressing mitogen-activated protein kinase kinase kinase 2

Contact Info

Product

Resources

About