Oxidative stress is involved in LLLT mechanism of action on skin healing in rats

Hartmann, Diane Duarte; Martins, Rodrigo Pereira; Silva, T.C. da; Stefanello, Sílvio Terra; Courtes, Aline Alves; Gonçalves, Débora Farina; Furtado, Andrezza Bond Vieira; Duarte, Bárbara Santos Luccas; Signori, Luís Ulisses; Soares, Félix Alexandre Antunes; Puntel, Gustavo Orione

doi:10.1590/1414-431x202010293

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…In addition to wavelength, power, pulse rate, pulse duration, interpulse interval, total irradiation time, intensity (power/area) and dose (power irradiation time/area irradiated) are among the important parameters in LLLT. The mechanism of action of this type of laser is through photothermal effects; however, LLLT usually does not cause noticeable temperature changes 134,135 …”

Section: Instrumental Methodsmentioning

confidence: 99%

“…The mechanism of action of this type of laser is through photothermal effects; however, LLLT usually does not cause noticeable temperature changes. 134 , 135 …”

Section: Instrumental Methodsmentioning

confidence: 99%

“…The mechanism of action of this type of laser is through photothermal effects; however, LLLT usually does not cause noticeable temperature changes. 134,135 The exact mechanism of this method is not known, but it seems that the technique reduces wound inflammation by reducing the number of chemicals produced by the cells and reducing enzymes related to pain and inflammation. The influential factors in this method are the ideal wavelength, radiation dose, duration, and location of the treatment.…”

Section: Low-level Laser Therapymentioning

confidence: 99%

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Emerging treatment strategies in wound care

Mirhaj

Labbaf

Tavakoli

et al. 2022

International Wound Journal

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Wound healing is a complex process in tissue regeneration through which the body responds to the dissipated cells as a result of any kind of severe injury. Diabetic and non-healing wounds are considered an unmet clinical need. Currently, different strategic approaches are widely used in the treatment of acute and chronic wounds which include, but are not limited to, tissue transplantation, cell therapy and wound dressings, and the use of an instrument. A large number of literatures have been published on this topic; however, the most effective clinical treatment remains a challenge. The wound dressing involves the use of a scaffold, usually using biomaterials for the delivery of medication, autologous stem cells, or growth factors from the blood. Antibacterial and anti-inflammatory drugs are also used to stop the infection as well as accelerate wound healing. With an increase in the ageing population leading to diabetes and associated cutaneous wounds, there is a great need to improve the current treatment strategies. This research critically reviews the current advancement in the therapeutic and clinical approaches for wound healing and tissue regeneration. The results of recent clinical trials suggest that the use of modern dressings and skin substitutes is the easiest, most accessible, and most cost-effective way to treat chronic wounds with advances in materials science such as graphene as 3D scaffold and biomolecules hold significant promise. The annual market value for successful wound treatment exceeds over $50 billion US dollars, and this will encourage industries as well as academics to investigate the application of emerging smart materials for modern dressings and skin substitutes for wound therapy.cells therapy, platelet therapy, skin tissue engineering, wound dressing, wound healing Key Messages• treatment of wound healing is currently considered an unmet clinical need • currently heavily under research are growth factors and cytokines released from platelets and leukocytes that have a significant effect on the cellular functions such as migration, differentiation, and proliferation, so they can regulate the wound healing process

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Section: Instrumental Methodsmentioning

confidence: 99%

“…The mechanism of action of this type of laser is through photothermal effects; however, LLLT usually does not cause noticeable temperature changes. 134 , 135 …”

Section: Instrumental Methodsmentioning

confidence: 99%

Section: Low-level Laser Therapymentioning

confidence: 99%

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Emerging treatment strategies in wound care

Mirhaj

Labbaf

Tavakoli

et al. 2022

International Wound Journal

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“…The red lights in the biophotonic sensor are placed in a hexagon for optimal light intensity and consider the overlapping of light to ensure effective light distribution. The selected red-light wavelength of 660 nm ± 5% [ 5 , 10 , 13 , 14 ] is absorbed well into the tissues, promoting metabolic cell activity and activating cell regeneration to treat skin disease [ 15 , 16 ]. The appearance applied to the skin can be confirmed through Figure 1 c.…”

Section: System and Algorithmmentioning

confidence: 99%

The Novel Digital Therapeutics Sensor and Algorithm for Pressure Ulcer Care Based on Tissue Impedance

Jung

Jang

Lee

2023

Sensors

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Visual diagnosis and rejuvenation are methods currently used to diagnose and treat pressure ulcers, respectively. However, the treatment process is difficult. We developed a biophotonic sensor to diagnose pressure ulcers and, subsequently, developed a pressure ulcer care device (PUCD.) We conducted animal and clinical trials to investigate the device’s effectiveness. We confirmed the accuracy of the pressure ulcer diagnosis algorithm to be 91% and we observed an 85% reduction in immune cells when using the PUCD to treat pressure ulcer-induced mice. Additionally, we compared the treatment group to the pressure ulcer induction group to assess the PUCD’s effectiveness in identifying immune cells through its nuclear shape. These results indicate a positive effect and suggest the use of PUCD as a recovery method for pressure ulcer diagnosis and treatment.

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“…It is believed that red and infrared (NIR) light is absorbed by chromophores, such as cytochrome c oxidase (CCO) in the mitochondria, and potentially by photoacceptors in the cell’s plasma membrane [ 14 ]. This absorption leads to the generation of reactive oxygen species (ROS) [ 15 , 16 ]. ROS, in turn, can activate various intracellular signaling pathways, modulate the affinity of transcription factors, and influence processes such as proliferation, cell survival, tissue repair, regeneration, and inflammation [ 13 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Laser Biostimulation Induces Wound Healing-Promoter β2-Defensin Expression in Human Keratinocytes via Oxidative Stress

Migliario,

Yerra,

Gino

et al. 2023

Antioxidants

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The innate immune system is the first line of defense of the body composed of anatomical barriers, such as skin and mucosa, as well as effector cells, antimicrobial peptides, soluble mediators, and cell receptors able to detect and destroy viruses and bacteria and to sense trauma and wounds to initiate repair. The human β-defensins belong to a family of antimicrobial small cationic peptides produced by epithelial cells, and show immunomodulatory and pro-healing activities. Laser biostimulation is a therapy widely used to contrast microbial infection and to accelerate wound healing through biological mechanisms that include the creation of oxidative stress. In this paper, we explored laser biostimulation’s ability to modulate the production of two β-defensins, hBD-1 and hBD-2, in human keratinocytes and whether this modulation was, at least in part, oxidative-stress-dependent. Human spontaneously immortalized keratinocytes (HaCaT) were stimulated using laser irradiation at a 980 nm wavelength, setting the power output to 1 W (649.35 mW/cm2) in the continuous mode. Cells were irradiated for 0 (negative control), 5, 10, 25 and 50 s, corresponding to an energy stimulation of 0, 5, 10, 25 and 50 J. Positive control cells were treated with lipopolysaccharide (LPS, 200 ng/mL). After 6 and 24 h of treatment, the cell conditioned medium was collected and analyzed via ELISA assay for the production of hBD-1 and hBD-2. In another set of experiments, HaCaT were pre-incubated for 45 min with antioxidant drugs—vitamin C (Vit. C, 100 µM), sodium azide (NaN3, 1 mM); ω-nitro-L-arginine methyl ester (L-NAME, 10 mM) and sodium pyruvate (NaPyr, 100 µM)—and then biostimulated for 0 or 50 s. After 6 h, the conditioned medium was collected and used for the ELISA analysis. The hBD-1 and hBD-2 production by HaCaT was significantly increased by single laser biostimulation after 6 h in an energy-dependent fashion compared to basal levels, and both reached production levels induced by LPS. After 24 h, only hBD-2 production induced by laser biostimulation was further increased, while the basal and stimulated hBD-1 levels were comparable. Pre-incubation with antioxidative drugs was able to completely abrogate the laser-induced production of both hBD-1 and hBD-2 after 6 h, with the exception of hBD-1 production in samples stimulated after NaN3 pre-incubation. A single laser biostimulation induced the oxidative-stress-dependent production of both hBD-1 and hBD-2 in human keratinocytes. In particular, the pro-healing hBD-2 level was almost three times higher than the baseline level and lasted for 24 h. These findings increase our knowledge about the positive effects of laser biostimulation on wound healing.

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Oxidative stress is involved in LLLT mechanism of action on skin healing in rats

Cited by 8 publications

References 37 publications

Emerging treatment strategies in wound care

Emerging treatment strategies in wound care

The Novel Digital Therapeutics Sensor and Algorithm for Pressure Ulcer Care Based on Tissue Impedance

Laser Biostimulation Induces Wound Healing-Promoter β2-Defensin Expression in Human Keratinocytes via Oxidative Stress

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