In vivo evidence of angiogenesis inhibition by β2-glycoprotein I subfractions in the chorioallantoic membrane of chicken embryos

Baldavira, Camila Machado; Gomes, Lígia Ferreira; Cruz, Leandro; Maria, Durvanei Augusto; Capelozzi, Vera Luíza

doi:10.1590/1414-431x202010291

Cited by 1 publication

(2 citation statements)

References 35 publications

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“…The effects of tissue samples upon angiogenesis were assayed in the chorioallantoic membrane of chicken embryo model [19]. Fifty-seven fertilized eggs (conventional, free from food contaminating human pathogens, Lohmann breed) (Yamaguishi farm, Sao Paulo, Brazil) were used.…”

Section: Angiogenesis and Embryo Developmentmentioning

confidence: 99%

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Differential Angiogenic Induction Impacts Nasal Polyp Tissue Growth

Góes

Sarafan

Amaral

et al. 2023

Indian J Otolaryngol Head Neck Surg

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Background: In chronic rhinosinusitis with nasal polyps, in ammatory edema drives tissue remodeling favoring anomalous growth of the nasal mucosa, but a proangiogenic contribution of nasal polyp in support of tissue growth is still controversial. The chorioallantoic membrane of chicken embryo model was employed to address the potentiality of nasal tissue fragments to modulate angiogenesis. Methods: Fifty-seven fertilized eggs were implanted with polyp or healthy nasal mucosa tissue or were kept as non-implanted controls. The embryos' size, length, and development stage, and chorioallantoic membrane vasculature morphology were evaluated after 48h. Quantitative computer vision techniques applied to digital chorioallantoic membrane images automatically calculated the branching index as the ratio between the areas of the convex polygon surrounding the vascular tree and the vessels' area.Results: Mucosal, but not polyp tissue implants, hampered embryo development and induced underdeveloped chorioallantoic membranes with anastomosed, interrupted, and regressive vessels.Vessels' areas and branching indexes were higher among the chorioallantoic membranes with polyp implants and controls than among those with healthy mucosa implants.Discuss: This study demonstrated for the rst time a differential angiogenic induction from nasal polyp tissue supporting tissue growth when compared with healthy nasal mucosa.Conclusion: Nasal polyp presents differential angiogenic induction that impacts tissue growth.

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Section: Angiogenesis and Embryo Developmentmentioning

confidence: 99%

“…The vessels' area in each ROI provides information about the growth and elongation of blood vessels, whereas branching index informed about the blood distribution and CAM area coverage. This provided complementary information about the angiogenesis processes taking place in the CAM membrane [19,22,23].…”

Section: Quantitative Morphologymentioning

confidence: 99%