Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents

Cristina, Romeo Teodor; Kocsis, Rudolf; Tulcan, Camelia; Alexa, Ersilia; Boldura, Oana Maria; Hulea, Călin; Dumitrescu, Eugenia; Radulov, Isidora; Муселин, Флорин

doi:10.1590/1414-431x20199001

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…While these methods only allow for a relative quantification of venom components, others like inductive coupled plasma (ICP) MS can be used for an absolute quantification, using the statistical abundance of cysteine sulfur in most venom proteins [ 54 , 55 ]. In addition, rather uncommon analytical tools have been used to investigate viper venoms, such as TD in-source decay (ISD) [ 56 ], venom on-a-chip [ 57 , 58 ], Fourier transform infrared spectroscopy (FTIR) [ 59 ], and the usage of a solid-phase combinatorial hexapeptide ligand library (CPLL) [ 60 ].…”

Section: Introductionmentioning

confidence: 99%

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Damm

Hempel

Süssmuth

2021

Toxins

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Fine-tuned by millions of years of evolution, snake venoms have frightened but also fascinated humanity and nowadays they constitute potential resources for drug development, therapeutics and antivenoms. The continuous progress of mass spectrometry techniques and latest advances in proteomics workflows enabled toxinologists to decipher venoms by modern omics technologies, so-called ‘venomics’. A tremendous upsurge reporting on snake venom proteomes could be observed. Within this review we focus on the highly venomous and widely distributed subfamily of Viperinae (Serpentes: Viperidae). A detailed public literature database search was performed (2003–2020) and we extensively reviewed all compositional venom studies of the so-called Old-World Vipers. In total, 54 studies resulted in 89 venom proteomes. The Viperinae venoms are dominated by four major, four secondary, six minor and several rare toxin families and peptides, respectively. The multitude of different venomics approaches complicates the comparison of venom composition datasets and therefore we differentiated between non-quantitative and three groups of quantitative workflows. The resulting direct comparisons within these groups show remarkable differences on the intra- and interspecies level across genera with a focus on regional differences. In summary, the present compilation is the first comprehensive up-to-date database on Viperinae venom proteomes and differentiating between analytical methods and workflows.

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Section: Introductionmentioning

confidence: 99%

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Damm

Hempel

Süssmuth

2021

Toxins

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“…The presence of blood coagulation and preventive proteins has been observed in snake venom. The effectiveness of these proteins is influenced by the amount and rate of venom entering the bloodstream [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Purification, and characterization of a new pro-coagulant protein from Iranian Echis carinatus venom

Khodadadi,

Rabiei,

Sardari

et al. 2024

Biochemistry and Biophysics Reports

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“…This method utilizes the Agilent 2,100 Bioanalyzer in combination with the protein LabChip kit, which simplifies the process of bioanalytical investigation and provides a system with standardized analysis handling and data processing ( 34 ). Although lab-on-chip cannot identify a polypeptide as a particular protein, the technology has been proven to be available for examining snake venom composition ( 35 ) and soybean cultivars in previous studies ( 36 ). It can perform molecular mass, migration time, peak height, peak area, relative concentration, and percentage of overall protein content and generate complete multi-peak spectrums of a sample.…”

Section: Introductionmentioning

confidence: 99%

Combining with lab-on-chip technology and multi-organ fusion strategy to estimate post-mortem interval of rat

Zhang

Long

et al. 2023

Front. Med.

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BackgroundThe estimation of post-mortem interval (PMI) is one of the most important problems in forensic pathology all the time. Although many classical methods can be used to estimate time since death, accurate and rapid estimation of PMI is still a difficult task in forensic practice, so the estimation of PMI requires a faster, more accurate, and more convenient method.Materials and methodsIn this study, an experimental method, lab-on-chip, is used to analyze the characterizations of polypeptide fragments of the lung, liver, kidney, and skeletal muscle of rats at defined time points after death (0, 1, 2, 3, 5, 7, 9, 12, 15, 18, 21, 24, 27, and 30 days). Then, machine learning algorithms (base model: LR, SVM, RF, GBDT, and MLPC; ensemble model: stacking, soft voting, and soft-weighted voting) are applied to predict PMI with single organ. Multi-organ fusion strategy is designed to predict PMI based on multiple organs. Then, the ensemble pruning algorithm determines the best combination of multi-organ.ResultsThe kidney is the best single organ for predicting the time of death, and its internal and external accuracy is 0.808 and 0.714, respectively. Multi-organ fusion strategy dramatically improves the performance of PMI estimation, and its internal and external accuracy is 0.962 and 0.893, respectively. Finally, the best organ combination determined by the ensemble pruning algorithm is all organs, such as lung, liver, kidney, and skeletal muscle.ConclusionLab-on-chip is feasible to detect polypeptide fragments and multi-organ fusion is more accurate than single organ for PMI estimation.

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Protein structure of the venom in nine species of snake: from bio-compounds to possible healing agents

Cited by 7 publications

References 36 publications

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Old World Vipers—A Review about Snake Venom Proteomics of Viperinae and Their Variations

Purification, and characterization of a new pro-coagulant protein from Iranian Echis carinatus venom

Combining with lab-on-chip technology and multi-organ fusion strategy to estimate post-mortem interval of rat

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