Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension

Marzuca‐Nassr, Gabriel Nasri; Fortes, Marco Aurélio Salomão; Guimarães‐Ferreira, Lucas; Murata, Gilson Masahiro; Vitzel, Kaio Fernando; Vasconcelos, Diogo Antônio Alves de; Bassit, R A; Curi, Rui

doi:10.1590/1414-431x20198391

Cited by 13 publications

(16 citation statements)

References 45 publications

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“…It has been reported that HS mimics spaceflight, bed rest, or the hospitalization state in humans [ 52 ]. Previous studies of our group [ 6 , 7 , 53 ] and others [ 61 , 62 ] described that this experimental model promotes a reduction of body weight, fat mass, muscle weight, and soleus CSA. Concomitantly, there are a drop of protein synthesis and a raise of protein degradation.…”

Section: Discussionmentioning

confidence: 80%

“…In this situation, the animals can still move using the forelimbs but do not use their hindlimbs. The same HS protocol was used in previous studies from the same laboratory [ 6 , 7 , 52 , 53 ].…”

Section: Methodsmentioning

confidence: 99%

“…There are several strategies to treat skeletal muscle atrophy [ 1 , 2 , 3 , 4 ]. One of them is dietary supplementation [ 5 , 6 ], including administration of fish oils (FOs) [ 7 ]. FOs contain high amounts of eicosapentaenoic (EPA) and docosahexaenoic (DHA), ω-3 fatty acids (FAs).…”

Section: Introductionmentioning

confidence: 99%

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Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

et al. 2021

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Endoplasmic reticulum stress (ERS) and autophagy pathways are implicated in disuse muscle atrophy. The effects of high eicosapentaenoic (EPA) or high docosahexaenoic (DHA) fish oils on soleus muscle ERS and autophagy markers were investigated in a rat hindlimb suspension (HS) atrophy model. Adult Wistar male rats received daily by gavage supplementation (0.3 mL per 100 g b.w.) of mineral oil or high EPA or high DHA fish oils (FOs) for two weeks. Afterward, the rats were subjected to HS and the respective treatments concomitantly for an additional two-week period. After four weeks, we evaluated ERS and autophagy markers in the soleus muscle. Results were analyzed using two-way analysis of variance (ANOVA) and Bonferroni post hoc test. Gastrocnemius muscle ω-6/ω-3 fatty acids (FAs) ratio was decreased by both FOs indicating the tissue incorporation of omega-3 fatty acids. HS altered (p < 0.05) the protein content (decreasing total p38 and BiP and increasing p-JNK2/total JNK2 ratio, and caspase 3) and gene expressions (decreasing BiP and increasing IRE1 and PERK) of ERS and autophagy (decreasing Beclin and increasing LC3 and ATG14) markers in soleus. Both FOs attenuated (p < 0.05) the increase in PERK and ATG14 expressions induced by HS. Thus, both FOs could potentially attenuate ERS and autophagy in skeletal muscles undergoing atrophy.

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Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

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Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

et al. 2021

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“…Dietary supplementation of creatine in tumor-bearing Wistar rats prevents muscle atrophy via alleviating inflammation and proteolysis as evidenced by reduced inflammation factor (TNF-α and IL-6) and muscle atrophy gene (MAFbx and MuRF1) [41]. In a separate study, Marzuca-Nassr et al reported that oral gavage of creatine to Wistar rats increases the muscle mass and the protein level of 4E-BP1 in extensor digitorum longus and prevents hindlimb suspension-induced muscle atrophy [42].…”

Section: Protein Amino Acid and Peptidesmentioning

confidence: 99%

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

Wang

Liu²,

Quan

et al. 2021

Nutrients

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Imbalance of protein homeostasis, with excessive protein degradation compared with protein synthesis, leads to the development of muscle atrophy resulting in a decrease in muscle mass and consequent muscle weakness and disability. Potential triggers of muscle atrophy include inflammation, malnutrition, aging, cancer, and an unhealthy lifestyle such as sedentariness and high fat diet. Nutraceuticals with preventive and therapeutic effects against muscle atrophy have recently received increasing attention since they are potentially more suitable for long-term use. The implementation of nutraceutical intervention might aid in the development and design of precision medicine strategies to reduce the burden of muscle atrophy. In this review, we will summarize the current knowledge on the importance of nutraceuticals in the prevention of skeletal muscle mass loss and recovery of muscle function. We also highlight the cellular and molecular mechanisms of these nutraceuticals and their possible pharmacological use, which is of great importance for the prevention and treatment of muscle atrophy.

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“…Under the in uence of MSTN receptor, muscle atrophy may occur [51]. Marzucanassr et al [52] found an increase in MSTN expression in the muscle of rats with hind limb unloading. In our previous hindlimb unloading model, LIPUS effectively inhibited the expression of MSTN in quadriceps femoris muscle of rats with muscular atrophy [53].Similarly, LIPUS signi cantly inhibited MSTN expression in type 1 diabetic rats, exercise-induced muscle hypertrophy rats and ovariectomized rats [14,17,54].In this study, LIPUS also signi cantly inhibited the expression of MSTN and its receptor at mRNA and protein levels, which may be related to the mechanism of LIPUS improving muscle atrophy.…”

Section: Disscusionmentioning

confidence: 99%

Low-intensity Pulsed Ultrasound Improves Muscle Atrophy in Hindlimb Unloading Rats and its Metabolic Mechanism

Sun¹,

An²,

Zhang³

et al. 2021

Preprint

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Low-intensity pulsed ultrasound (LIPUS) has been proved to promote the proliferation of myoblast C2C12. However, whether LIPUS can effectively prevent muscle atrophy has not been clarified, and if so, what is the possible mechanism. Myostatin (MSTN) is a negtive regulator of skeletal muscle, and inhibition of its expression has a positive effect on the growth and development of skeletal muscle.The aim of this study is to evaluate the effects of LIPUS on muscle atrophyin hind limb unloading rats, and explored the mechanisms. The rats were randomly divided into four groups: normal control group (NC), hind limb unloading group (UL), hind limb unloading plus 30 mW/cm2 irradiation group (UL + 30 mW/cm2), hind limb suspension plus 80 mW/cm2 irradiation group (UL + 80 mW/cm2). The rats were suspended or/and treated with LIPUS for 20 min/d for 28 days. C2C12 cells were exposed to LIPUS at 30 or 80 mW/cm2 for 5 days. After 28 days, LIPUS significantly prevented the decrease of cross-sectional area of muscle fiber and promoted the quality of gastrocnemius muscle. In addition, LIPUS significantly inhibited the content of MSTN in the serum and gastrocnemius muscle of hind limb rats, and its receptor, and promoted myoblast C2C12 proliferation, promoted the stability of alanine, aspartate and glutamate metabolism pathway. These results suggest that the key mechanism of LIPUS in preventing muscle atrophy induced by hind limb unloading may be through inhibiting MSTN and stabilizing alanine, aspartate and glutamate metabolism.

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Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension

Cited by 13 publications

References 45 publications

Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

Endoplasmic Reticulum Stress and Autophagy Markers in Soleus Muscle Disuse-Induced Atrophy of Rats Treated with Fish Oil

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

Low-intensity Pulsed Ultrasound Improves Muscle Atrophy in Hindlimb Unloading Rats and its Metabolic Mechanism

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