Transcriptional profiling of uterine leiomyoma rats treated by a traditional herb pair, Curcumae rhizoma and Sparganii rhizoma

Abstract

The aim of this study was to elucidate the concise effects of a traditional herb pair, Curcumae rhizoma-Sparganii rhizoma (CRSR), on uterine leiomyoma (UL) by analyzing transcriptional profiling. The UL rat model was made by intramuscular injection of progesterone and gavage administration of diethylstilbestrol. From 11 weeks of the establishment of the model, rats of the UL+CRSR group were gavaged daily with CRSR (6.67 g/kg). The serum concentrations of progesterone (P) and estradiol (E 2 ) were determined by radioimmunoassay, the uterine index was measured by caliper measurement, and the pathological status was observed by hematoxylin and eosin stain. Gene expression profiling was checked by NimbleGen Rat Gene Expression Microarrays. The results indicated that the uterine mass of UL+CRSR rats was significantly shrunk and serum P and E 2 levels significantly reduced compared to UL animals and nearly to the level of normal rats. Results of microarrays displayed the extensive inhibition of CRSR upon the expression of proliferation and deposition of extracellular matrix (ECM)-related genes, and significantly regulated a wide range of metabolism disorders. Furthermore, CRSR extensively regulated key pathways of the UL process, such as MAPK, PPAR, Notch, and TGF-β/Smad. Regulation of the crucial pathways for the UL process and ECM metabolism may be the underlying mechanisms of CRSR treatment. Further studies will provide clear clues for effectively treating UL with CRSR.

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“…40 Also, 95% and 60% ethanol extraction, respectively, were used in two of the studies. [41][42] The methods of treatment administration also varied from intragastrical/gavage [42][43] to intraperitoneal. 41 In some of these studies, more than one plant was used jointly, whether as a measured concoction, or a mixture to test the synergistic effects of the combined plants.…”

“…From this review, Anthocyanins, 50 Quercetin, indole-3-carbinol, 100 and joint Curcumae rhizoma-Sparganii rhizoma (CRSR) herb extracts inhibited ECM components (fibronectin, versican, and collagen1A1). 42 Resveratrol also regulated fibronectin, © 2021 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License α-SMA, vimentin, COL1A1, and β-catenin proteins in vitro by interacting with these proteins, thus resulting in a downregulated expression.…”

“…49 CRSR combined extracts also regulated important fibroid signaling pathways: TGFbeta/Smad, MAPK, and PPAR signaling pathways. 42…”

Inhibition of Fibroblast Activation by Components of Rhizoma Curcumae and Rhizoma Sparganii in A Rat Model of Uterine Leiomyoma

“…Our previous studies have shown that the therapeutic effects of RCRS on UL include improving pathological status, reducing volume, and inhibiting myoma cell proliferation (Yu et al, 2014). Another study showed that the therapeutic effect of RCRS on UL may be related to the regulation of endocrine levels (Yu et al, 2019). The results of metabolomics prove that RCRS can reverse the abnormal metabolism in rats.…”

Inhibition of Fibroblast Activation in Uterine Leiomyoma by Components of Rhizoma Curcumae and Rhizoma Sparganii

“…Our previous studies have confirmed that the therapeutic effects of RCRS on UL include improvement of pathological status, reduction of volume, and inhibition of myoma cell proliferation ( 7 ). Another study showed that the therapeutic effect of RCRS on UL might be related to the regulation of endocrine levels ( 8 ). The results of a metabolomics study confirmed that RCRS can reverse abnormal metabolism in rats.…”