NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway

Li, Jingrong; Fang, Ruihua; Deng, Liehua

doi:10.1590/1414-431x20187952

Cited by 21 publications

(29 citation statements)

References 22 publications

(22 reference statements)

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“…A previous study demonstrated that NOP14 enhances the stability of mutp53, enabling mutp53 to participate in the progression of pancreatic cancer . NOP14 could suppress cancer metastasis by regulating the Wnt/β‐catenin signalling pathway in cancers . In our research, we found that NOP14 is involved in cell proliferation and migration and induces apoptosis in BCa cells.…”

Section: Discussionsupporting

confidence: 60%

“…35 NOP14 could suppress cancer metastasis by regulating the Wnt/β-catenin signalling pathway in cancers. 36,37 In our research, we found that NOP14 is involved in cell proliferation and migration and induces apoptosis in BCa cells. Furthermore, NOP14 silencing reduced the expression of cell cycle-related proteins and MMP9 and upregulated the expression of cleaved Caspase 3.…”

Section: Discussionmentioning

confidence: 59%

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CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer

Ying

Xie

et al. 2020

Cell Proliferation

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Objectives Downregulation of miR‐502‐5p has emerged as a critical factor in tumour progression in several cancers. Herein, we elucidated the role of miR‐502‐5p in bladder cancer. Materials and methods RT‐qPCR was performed to examine the expression of miR‐502‐5p in bladder cancer. And DNA methylation analysis showed that epigenetic mechanisms may contribute to the downregulation of miR‐502‐5p. Then, wound‐healing assay, transwell assay, colony formation assay, CCK8 assay and flow cytometry analysis were applied to evaluate the function of miR‐502‐5p in bladder cancer cell lines. Western blot was conducted to measure the protein levels of related genes. Furthermore, dual‐luciferase reporter assay, in vivo tumorigenesis assay and immunohistochemical staining were also conducted as needed. Results MiR‐502‐5p is frequently downregulated in BCa. Meanwhile, hypermethylation of CpG islands contributes to the downregulation of miR‐502‐5p. Functionally, overexpression of miR‐502‐5p inhibited cell proliferation and migration in vitro and repressed tumour growth in vivo. CCND1, DNMT3B and NOP14 were identified as direct targets of miR‐502‐5p. Interestingly, DNMT3B and miR‐502‐5p established a positive feedback loop in the regulation of bladder cancer. In addition, rescue experiments further validated the direct molecular interaction between miR‐502‐5p and its targets. Conclusions Our study proposed and demonstrated that the miR‐502‐5p–mediated regulatory network is critical in bladder cancer; this network may be useful in the development of more effective therapies against bladder cancer.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 59%

CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer

Ying

Xie

et al. 2020

Cell Proliferation

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“…By inhibiting the Wnt/ β-catenin pathways, NOP14 suppresses breast cancer [49]. In addition, NOP14 can reduce melanoma cell proliferation and metastasis by regulating the Wnt/b-catenin signaling pathway30484495 [50]. It was found out in this study that the expression of NOP14 was upregulated in CRC, which means its pathogenesis requires further research and con rmation.…”

Section: Discussionmentioning

confidence: 78%

Integrated analysis of RNA binding proteins in human colorectal cancer

Fan

Liu

Shi

et al. 2020

Preprint

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Background: Though RNA-binding proteins play an essential role in a variety of different tumors, there is still limited effort made on the systematic analysis of RNA binding proteins (RBPs) in the survival of colorectal cancer(CRC).Methods: An analysis was conducted of the CRC transcriptome data collected from the TCGA database and RBPs were extracted from CRC. R software was applied to analyze the differentially expressed genes (DEGs) of RBPs. To identify the pathways and functional annotation of RBPs DEGs, Gene Ontology (GO) function, Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were carried out using the database for annotation, visualization and integrated discovery. The interactive gene database search tool was employed to conduct protein-protein interaction analysis, while Cytoscape software was applied for visual processing. Based on the COX regression analysis of the prognostic value of interacting RBPs and survival time, the RBPs related to survival were screened out, and a prognostic model was constructed. The data stored in TCGA database was taken as the Train group, while the chip data obtained from the GEO database was treated as the Test group to verify the model. Then, both survival analysis and ROC curve verification were conducted. Finally, the risk curves and nomograms of the two groups were drawn to predict the survival period.Results: There were 314 genes up-regulated by RBPs DEGs and 155 genes down-regulated, from which twelve RBPs (NOP14, MRPS23, MAK16, TDRD6, POP1, TDRD5, TDRD7, PPARGC1A, LIN28B, CELF4, LRRFIP2, MSI2) with prognostic markers were obtained.Conclusions: These twelve genes may be applicable as the predictor of CRC and play an essential role in the pathogenesis of CRC. In spite of this, it remains necessary to further explore the underlying mechanism.

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“…By inhibiting the Wnt/β-catenin pathways, NOP14 suppresses breast cancer [67]. In addition, NOP14 can reduce melanoma cell proliferation and metastasis by regulating the Wnt/b-catenin signalling pathway [68]. In clinical studies of patients with ovarian cancer, downregulation of NOP14 was associated with a significantly worse survival rate [69].…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of RNA-binding proteins in human colorectal cancer

et al. 2020

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Background: Although RNA-binding proteins play an essential role in a variety of different tumours, there are still limited efforts made to systematically analyse the role of RNA-binding proteins (RBPs) in the survival of colorectal cancer (CRC) patients. Methods: Analysis of CRC transcriptome data collected from the TCGA database was conducted, and RBPs were extracted from CRC. R software was applied to analyse the differentially expressed genes (DEGs) of RBPs. To identify related pathways and perform functional annotation of RBP DEGs, Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out using the database for annotation, visualization and integrated discovery. Protein-protein interactions (PPIs) of these DEGs were analysed based on the Search Tool for the Retrieval of Interacting Genes (STRING) database and visualized by Cytoscape software. Based on the Cox regression analysis of the prognostic value of RBPs (from the PPI network) with survival time, the RBPs related to survival were identified, and a prognostic model was constructed. To verify the model, the data stored in the TCGA database were designated as the training set, while the chip data obtained from the GEO database were treated as the test set. Then, both survival analysis and ROC curve verification were conducted. Finally, the risk curves and nomograms of the two groups were generated to predict the survival period. Results: Among RBP DEGs, 314 genes were upregulated while 155 were downregulated, of which twelve RBPs (NOP14, MRPS23, MAK16, TDRD6, POP1, TDRD5, TDRD7, PPARGC1A, LIN28B, CELF4, LRRFIP2, MSI2) with prognostic value were obtained. Conclusions: The twelve identified genes may be promising predictors of CRC and play an essential role in the pathogenesis of CRC. However, further investigation of the underlying mechanism is needed.

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NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway

Cited by 21 publications

References 22 publications

CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer

CCND1, NOP14 and DNMT3B are involved in miR‐502‐5p–mediated inhibition of cell migration and proliferation in bladder cancer

Integrated analysis of RNA binding proteins in human colorectal cancer

Integrated analysis of RNA-binding proteins in human colorectal cancer

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