Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion

Paim, Ágata; Braghirolli, Daikelly Iglesias; Cardozo, Nilo Sérgio Medeiros; Pranke, Patrícia; Tessaro, Isabel Cristina

doi:10.1590/1414-431x20186754

Cited by 6 publications

(5 citation statements)

References 37 publications

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“…When analyzing the pore size of the scaffold by SEM, a structure of micropores and macropores was observed with dimensions ranging from 73 µm to 124 µm and with an average size of 86 µm ( Figure 1 B). A crucial characteristic of a scaffold is the ability to have interconnected pores, which is favorable for cell adhesion and growth; however, extremely large pores can impair the vascularization of the biomaterial by making difficult contact between cells [ 41 ]. Majore et al [ 42 ] reported how the size of mesenchymal cells could vary from 11 to 19 µm and suggested how the size of the pores, presented in the scaffold developed, could be sufficient for cell growth, allowing the exchange of nutrients and metabolites between permeated cells within the material.…”

Section: Resultsmentioning

confidence: 99%

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Leite

Oliveira²,

Quelemes³

et al. 2023

Pharmaceuticals

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Hydrogels are structures that have value for application in the area of tissue engineering because they mimic the extracellular matrix. Naturally obtained polysaccharides, such as chitosan (CH) and cashew gum, are materials with the ability to form polymeric networks due to their physicochemical properties. This research aimed to develop a scaffold based on chitosan and phthalated cashew tree gum and test it as a support for the growth of human mesenchymal stem cells. In this study, phthalation in cashew gum (PCG) was performed by using a solvent-free route. PCG-CH scaffold was developed by polyelectrolyte complexation, and its ability to support adherent stem cell growth was evaluated. The scaffold showed a high swelling rate. The pore sizes of the scaffold were analyzed by scanning electron microscopy. Human dental pulp stem cells (hDPSCs) were isolated, expanded, and characterized for their potential to differentiate into mesenchymal lineages and for their immunophenotypic profile. Isolated mesenchymal stem cells presented fibroblastoid morphology, plastic adhesion capacity, and differentiation in osteogenic, adipogenic, and chondrogenic lineages. Mesenchymal stem cells were cultured in scaffolds to assess cell adhesion and growth. The cells seeded on the scaffold showed typical morphology, attachment, and adequate distribution inside the matrix pores. Thus, cells seeded in the scaffold may improve the osteoinductive and osteoconductive properties of these biomaterials.

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Section: Resultsmentioning

confidence: 99%

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Leite

Oliveira²,

Quelemes³

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…138 In addition, some more complex dynamic mechanical behaviors play very important regulatory roles in the stem cell microenvironment maintenance, thus how to achieve accurate detection of these mechanical parameters is also the research direction of scientists. 139,140 4.2.3. Biological Factors.…”

Section: Acs Applied Bio Materialsmentioning

confidence: 99%

“…Equivalently, not only did the polylysine and 19-mer peptide bifunctional hydrogels prepared by Farrukh regulate the stiffness of the scaffolds by adjusting the proportions of the two components, thus promoting the differentiation of stem cells to varying degrees, but also it promoted the generation of β1-integrin in embryonic cortical neurons by the components synergy . In addition, some more complex dynamic mechanical behaviors play very important regulatory roles in the stem cell microenvironment maintenance, thus how to achieve accurate detection of these mechanical parameters is also the research direction of scientists. , …”

Section: Bioreactor Synergy With Scaffolds For Stem Cells Proliferati...mentioning

confidence: 99%

Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

Huang

Liu

et al. 2018

ACS Appl. Bio Mater.

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Because of unparalleled advantages over other cells, stem cells are widely used in genetic diagnosis, drug delivery, and regenerative medicine. However, because the content of stem cells in the organism is far from satisfactory, it is of great significance of stem cells to in vitro proliferation and differentiation. However, many stem cell cultures have low expansion efficiency and stem cells lose their value-adding ability and differentiation ability after many generations of culture. To solve these problems, people hope to more realistically simulate the microenvironment in which stem cells grow in vivo. Cell scaffolds gradually evolve from 2D structures to 3D structures. The addition of growth factors influences cell behavior from internal biochemical conditions and the development of smart bioreactors gradually make progress to more precise regulate the external conditions of stem cell. In this paper, the key factors for constructing the microenvironment of stem cell growth were analyzed, and we reviewed the application of bioreactors and 3D scaffolds in stem cell cultivation. Finally, this paper indicated the development directions of stem cell culture in vitro.

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“…MSCs from exfoliated deciduous teeth were used in association with polycaprolactone electrospun scaffolds to study the effect of flow rate and shear stress, adhesion time and seeding density under direct perfusion in a bioreactor [ 21 ]. MSC derivates of exfoliated deciduous teeth modulated early astrocyte response after spinal cord contusion [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

2023

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Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber to house the organ coupled to a peristaltic pump to promote liquid flow through the organ vascular tree. The rats’ liver decellularization was made with a solution of sodium dodecyl sulfate. The recellularization was made with 108 mesenchymal stromal/stem cells and cultivated for seven days. The decellularized matrices showed an absence of DNA while preserving the collagen and glycosaminoglycans quantities, confirming the efficiency of the process. The functional analyses showed a rise in lactate dehydrogenase levels occurring in the first days of the cultivation, suggesting that there is cell death in this period, which stabilized on the seventh day. Histological analysis showed conservation of the collagen web and some groups of cells next to the vessels. It was possible to establish a system for decellularization and a bioreactor to use for the recellularization method. It is easy to assemble, can be ready to use in little time and be easily sterilized.

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Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion

Cited by 6 publications

References 37 publications

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Novel Scaffold Based on Chitosan Hydrogels/Phthalated Cashew Gum for Supporting Human Dental Pulp Stem Cells

Bioreactor Synergy with 3D Scaffolds: New Era for Stem Cells Culture

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

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