Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats

Ribeiro, L.P.; Freitas-Lima, Leandro Ceotto; Naumann, Gustavo B.; Meyrelles, Silvana S.; Lunz, Wellington; Pires, Simone da Fonseca; Andrade, Hélida Monteiro de; Carnielli, Juliana B.T.; Figueiredo, Suely G.

doi:10.1590/1414-431x20177033

Cited by 3 publications

(2 citation statements)

References 39 publications

(60 reference statements)

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“…According to the methods from literatures[11], a little change is made.At 2 days after training, the load and movement of the rats were increased. The following protocol was applied: running at approximately 5 m/min, 10 min after warming up, followed by a starting load at 10 m/min, with a gradual increase over 3 min, followed by 5 m/min, until exhaustion.…”

Section: Methodsmentioning

confidence: 99%

Netrin-1 plays a role in the effect of moderate exercise on myocardial fibrosis in rats

Zhou¹,

Hong³

et al. 2019

PLoS ONE

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Introduction This study aimed to investigate the effect of aerobic exercise on the expression of neitrin-1,DCC receptor and myocardial fibrosis in rats with acute myocardial infarction. Methods Twenty-four rats were randomly divided into three groups: the sham group (n = 8), the acute myocardial infarction (AMI) model group (n = 8), and the aerobic exercise treatment after acute myocardial infarction group (ET) (n = 8). After 10 weeks, the serum levels of netrin-1, tumor necrosis factor alpha α (TNF-α), and interleukin 6 (IL-6) were measured. The expression of matrix metalloproteinase 2 and 9 (MMP2, 9), and their inhibitor, tissue inhibitor of metalloproteinase 2 (TIMP2), myocardial netrin-1, and the deleted in colorectal cancer (DCC) receptor were evaluated. Histopathological results were also evaluated. The collagen volume fraction of the myocardial tissues was also calculated. Results Compared with the sham group, in the AMI and ET groups, left ventricular end diastolic pressure (LVEDP) were increased, while left ventricular systolic pressure (LVSP), and left ventricular pressure maximal rate of rise and fall (± dp/dtmax) were significantly decreased (P<0.05,). Compared with the AMI group, in the ET group, LVSP, and ±dp/dtmax were significantly increased while LVEDP was decreased (P<0.05). Compared with the sham group, the AMI group and ET groups showed increased levels of serum TNF-α, IL-6 and significantly reduced levels of netrin-1. Levels of TNF-α and IL-6 were significantly reduced in the ET group compared with the AMI group, whereas the level of netrin-1 was increased. The expression of myocardial MMP2 and MMP9 was significantly increased in the AMI group compared with the sham group, whereas that of myocardial netrin-1, TIMP2 and the DCC receptor, was significantly reduced. Compared with the AMI group, the ET group showed reduced expression of myocardial MMP2 and MMP9 proteins, whereas expression of myocardial netrin-1, TIMP2 and the DCC receptor, was significantly increased. The collagen volume fraction of the myocardial tissues was significantly increased in the AMI group and the ET group compared with the sham group, with a greater increase in the AMI group. Conclusions Aerobic exercise increased levels of serum netrin-1, myocardial netrin-1, and the DCC receptor and reduced the expression of myocardial MMP2 and MMP9 proteins, to improve the degree of fibrosis following myocardial infarction in rats.

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Section: Methodsmentioning

confidence: 99%

Netrin-1 plays a role in the effect of moderate exercise on myocardial fibrosis in rats

Zhou¹,

Hong³

et al. 2019

PLoS ONE

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“…Regarding the cardiac muscle, it has been shown that the basal level of HSPB5 protein is higher in the heart of rats with low running performance, associated with an increase of other proteins related to stress response [ 150 ], while the only paper specifically addressing the exercise-induced modulation of HSPB5 shows that acute endurance exercise does not determine any early upregulation of this protein in mouse heart [ 63 ].…”

Section: Hspb5 Modulation By Physical Activity/exercisementioning

confidence: 99%

Alpha B-Crystallin in Muscle Disease Prevention: The Role of Physical Activity

Dimauro

Caporossi

2022

Molecules

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HSPB5 or alpha B-crystallin (CRYAB), originally identified as lens protein, is one of the most widespread and represented of the human small heat shock proteins (sHSPs). It is greatly expressed in tissue with high rates of oxidative metabolism, such as skeletal and cardiac muscles, where HSPB5 dysfunction is associated with a plethora of human diseases. Since HSPB5 has a major role in protecting muscle tissues from the alterations of protein stability (i.e., microfilaments, microtubules, and intermediate filament components), it is not surprising that this sHSP is specifically modulated by exercise. Considering the robust content and the protective function of HSPB5 in striated muscle tissues, as well as its specific response to muscle contraction, it is then realistic to predict a specific role for exercise-induced modulation of HSPB5 in the prevention of muscle diseases caused by protein misfolding. After offering an overview of the current knowledge on HSPB5 structure and function in muscle, this review aims to introduce the reader to the capacity that different exercise modalities have to induce and/or activate HSPB5 to levels sufficient to confer protection, with the potential to prevent or delay skeletal and cardiac muscle disorders.

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