Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model

Guo, Xiangjun; Bai, Zhaofang; Qiang, Bin; Bu, Fanping; Zhao, Nange

doi:10.1590/1414-431x20165625

Cited by 5 publications

(5 citation statements)

References 29 publications

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“…Geng Guangqi et al; used the ventral transverse process approach to expose the vertebral body and intervertebral disc, and they successfully established a model establishment by drilling a hole in the upper endplate of the vertebral body and, filling it with gelfoam sponge infused with H37Rv standard M. tuberculosis suspension. The New Zealand rabbit model of spinal tuberculosis using the same or similar methods has been used in basic research related to spinal tuberculosis with satisfactory results 15 , 16 . In previous in vivo experiments using this method, the results showed that repeated surgeries caused local adhesion, unclear anatomical structure boundaries, and a corresponding increase in the incidence of surgical complications such as peritoneal rupture.…”

Section: Discussionmentioning

confidence: 99%

A comparative study of a rabbit spinal tuberculosis model constructed by local direct infection via the posterior lateral approach

Yue

Zhu

et al. 2022

Sci Rep

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The present study aims to establish a method of constructing a New Zealand rabbit spinal tuberculosis model by direct local infusion of M. tuberculosis H37Rv strain into the intervertebral disc space through the posterior lateral approach. Sixty-six New Zealand rabbits were pretreated with complete Freund's adjuvant and randomly divided into 4 group: the posterolateral approach model group (Group A, 25), ventral transverse process approach model group (Group B, 25), control group (Group C, 10), and blank group (Group D, 6). In Groups A and B, the bone holes were filled with gelatin sponge after drilling, and the local area was directly infused with 0.1 ml of M. tuberculosis H37Rv strain suspension. In Group C, the gelatin sponge was filled through the posterolateral approach and the local area was infused with 0.1 ml of normal saline suspension. In Group D, No specific treatment was performed. The general conditions of the experimental rabbits in each group were compared to those of a control group; the degree of vertebral body exposure, incision length, and complications of the two methods were compared; and the tuberculosis models were evaluated by imaging, histopathology, and bacterial culture. In Group A, the lateral side of the vertebral body was well exposed, the damage was mild, and no peritoneal rupture or gastrointestinal complications were observed. In Group B, the ventral side of the vertebral body and the intervertebral disc were exposed, and abdominal complications were more likely to occur. The survival rates of the experimental rabbits at 8 weeks after surgery were 92.0% in Group A, 88.00% in Group B, 90.0% in Group C, and 100% in Group D. MRI examinations showed that in Group A, the positive rate of radiographic bone findings was 86.9% at 4 weeks after surgery and 100% at 8 weeks after surgery; in Group B, the positive rate of radiographic bone findings was 78.2% at 4 weeks after surgery and 95.4% at 8 weeks after surgery. There was no significant difference between Groups A and B in the radiographic bone findings rate detected by the same imaging method at the same time point (P > 0.05). Eight weeks after surgery, bone destruction, paravertebral abscess, and caseous necrosis occurred in the vertebral bodies of surviving rabbits in Groups A and B. The BacT/ALERT 3D rapid culture system was used to culture the pus in the lesion, and the results showed that the positive rate of tuberculosis was 52.17% in Group A and 54.54% in Group B, and the difference was not statistically significant (P > 0.05). After pretreatment with complete Freund's adjuvant, direct infusion of the H37Rv strain of M. tuberculosis into the intervertebral disc space of New Zealand rabbits via the posterolateral approach and the ventral transverse process approach can successfully establish rabbit spinal tuberculosis models.

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Section: Discussionmentioning

confidence: 99%

A comparative study of a rabbit spinal tuberculosis model constructed by local direct infection via the posterior lateral approach

Yue

Zhu

et al. 2022

Sci Rep

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“…ST is the most occurred non-pulmonary tuberculosis in China, which account for more than 50% of total cases [ 18 ]. The progression of disease could finally lead to spinal deformity, neural dissects and even paraplegia, which are irreversible damages not directly caused by the Mtb infection but prolonged inflammatory responses surrounding the infected tissues [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…In previous research, the expression of NF-κB subtype p65 has been shown elevated in lymphocytes in New Zealand white rabbits with ST [ 18 ]. NF-κB is potent in inflammatory responses in most diseases, however, the overexpression of it might be deleterious.…”

Section: Discussionmentioning

confidence: 99%

“…Tumor necrosis factor (TNF), the activator of NF-κB, has been shown essential in restricting the growth of bacteria in granulomas [ 12 16 17 ]. In previous study, the expression of MCP-1 and NF-κB are both upregulated in New Zealand rabbits carrying ST [ 18 ]. Hence, both of the proteins can be important targets in attenuating the inflammatory effects in ST.…”

Section: Introductionmentioning

confidence: 99%

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Isoliquiritigenin attenuates spinal tuberculosis through inhibiting immune response in a New Zealand white rabbit model

Wang

Yang

Cui³

et al. 2018

Korean J Physiol Pharmacol

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Spinal tuberculosis (ST) is the tuberculosis caused by Mycobacterium tuberculosis (Mtb) infections in spinal curds. Isoliquiritigenin 4,2′,4′-trihydroxychalcone, ISL) is an anti-inflammatory flavonoid derived from licorice (Glycyrrhiza uralensis), a Chinese traditional medicine. In this study, we evaluated the potential of ISL in treating ST in New Zealand white rabbit models. In the model, rabbits (n=40) were infected with Mtb strain H37Rv or not in their 6th lumbar vertebral bodies. Since the day of infection, rabbits were treated with 20 mg/kg and 100 mg/kg of ISL respectively. After 10 weeks of treatments, the adjacent vertebral bone tissues of rabbits were analyzed through Hematoxylin-Eosin staining. The relative expression of Monocyte chemoattractant protein-1 (MCP-1/CCL2), transcription factor κB (NF-κB) p65 in lymphocytes were verified through reverse transcription quantitative real-time PCR (RT-qPCR), western blotting and enzyme-linked immunosorbent assays (ELISA). The serum level of interleukin (IL)-2, IL-4, IL-10 and interferon γ (IFN-γ) were evaluated through ELISA. The effects of ISL on the phosphorylation of IκBα, IKKα/β and p65 in NF-κB signaling pathways were assessed through western blotting. In the results, ISL has been shown to effectively attenuate the granulation inside adjacent vertebral tissues. The relative level of MCP-1, p65 and IL-4 and IL-10 were retrieved. NF-κB signaling was inhibited, in which the phosphorylation of p65, IκBα and IKKα/β were suppressed whereas the level of IκBα were elevated. In conclusion, ISL might be an effective drug that inhibited the formation of granulomas through downregulating MCP-1, NF-κB, IL-4 and IL-10 in treating ST.

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“…12 miRNA-155 is currently recognized as a miRNA involved in many biological processes, such as infection and in ammation, 13 which plays an important regulatory role in T cell-dependent immune response. 14 The previous studies found that the expression of miRNA-155 in patients with acute PTB was obviously increased, 15 which could up-regulate the early immune response of MTB infection. 16 Otherwise, the results showed that the expression of miRNA-155 in the serum of initial PTB patients was signi cantly lower than that in the healthy control group.…”

Section: Target-gene Interaction Network Predictionmentioning

confidence: 98%

Bioinformatic analysis of the microRNA-155 in pulmonary tuberculosis

Zhao

Chen

Zhang

et al. 2022

Preprint

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BACKGROUND: Tuberculosis is a chronic and infectious disease caused by Mycobacterium Tuberculosis.To identify the target genes of microRNA-155(miRNA-155) and their functional mechanisms by bioinformatic analysis. METHODS: Precursor sequences of miRNA-155 were obtained using the DNAMAN. Target genes were predicted through targetscan, PicTar and MiRDB database. Additionally, the functional enrichment analysis and Reactome enrichment analysis were performed to annotate the gene functions. RESULTS: Mature gene sequences of miRNA-155 family were highly conserved among various species. 49 target genes obtained from multiple databases were found to be enriched in membrane- enclosed lumen, negative regulation of cellular process, intracellular signal transduction and so on. Besides, the target genes were also seemed to be enriched in checkpoint kinase 1/ checkpoint kinase 2（Chk1/chk2） mediated inactivation of cyclin B:CDK1 complex (cyclin- dependent protein kinases). ACTL6A, SMARCA4, ACTB, and other genes might be closely related to the onset of tuberculosis. CONCLUSIONS: miRNA-155 target genes are involved in multiple biological processes and possibly participate in the pulmonary tuberculosis (PTB) pathogenesis by regulating immune-related genes, which may be used as potential biological markers for the research on PTB.

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Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model

Cited by 5 publications

References 29 publications

A comparative study of a rabbit spinal tuberculosis model constructed by local direct infection via the posterior lateral approach

A comparative study of a rabbit spinal tuberculosis model constructed by local direct infection via the posterior lateral approach

Isoliquiritigenin attenuates spinal tuberculosis through inhibiting immune response in a New Zealand white rabbit model

Bioinformatic analysis of the microRNA-155 in pulmonary tuberculosis

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