Cardioprotection against experimental myocardial ischemic injury using cornin

Abstract

Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and reperfusion (I/R) injury in vivo, and elucidated its potential mechanism in vitro. Effects of cornin on cell viability, as well as expression of phospho-CREB and phospho-Akt in hypoxic H9c2 cells in vitro, and myocardial I/R injury in vivo, were investigated. Cornin attenuated hypoxia-induced cytotoxicity significantly in H9c2 cells in a concentration-dependent manner. Treatment of H9c2 cells with cornin (10 µM) blocked the reduction of expression of phospho-CREB and phospho-Akt in a hypoxic condition. Treatment of rats with cornin (30 mg/kg, iv) protected them from myocardial I/R injury as indicated by a decrease in infarct volume, improvement in hemodynamics, and reduction of severity of myocardial damage. Cornin treatment also attenuated the reduction of expression of phospho-CREB and phospho-Akt in ischemic myocardial tissue. These data suggest that cornin exerts protective effects due to an increase in expression of phospho-CREB and phospho-Akt.

Preventive effect of Agnucastoside C against Isoproterenol-induced myocardial injury

“…and xylazine (10 mg/kg, i.m.) 13 , needle electrodes were inserted into paw pads, and connected to Cardiart 1081 (BPL) ECG machine. ECG recordings were made using 20 mm/mV sensitivity with a paper speed of 50 mm/s in lead II position and ST-segment elevation (expressed in mV) in normal and experimental animals were recorded.…”

“…Our earlier studies also reported that active components of its leaves exhibit cardio protection 11 , 12 . As in some reports, iridoid glycosides such as Cornin, Picroside II and Catalpol were also shown to be effective in cardio protection 13 – 15 , in this investigation we thought of isolating an iridoid glycoside from the methanolic extract of its leaves and then to investigate its preventing effects on myocardial injury, if any. On the basis of its spectroscopic analyses, as described earlier in previous report 16 , the isolated compound was identified as agnucastoside-C (7-O-trans-p-coumaroyl-6-O-trans-caffeoyl-8-epiloganic acid).…”

The microRNA‐193a‐5p induced ROS production and disturbed colocalization between STAT3 and androgen receptor play critical roles in cornin induced apoptosis

“…Cornin, also known as verbenalin, an iridoid monoglucoside, derived from Cornus officinalis (Choi et al, 2011), or Verbena officinalis (Xu et al, 2016), has been reported to induce apoptosis, inhibit the migration and invasion (Liao et al, 2015) and attenuate BPH (Hwangbo et al, 2018). Nevertheless, the antitumor mechanism of cornin has not been clearly determined in PCs to date.…”

Cornin protects against cerebral ischemia/reperfusion injury by preventing autophagy via the PI3K/Akt/mTOR pathway

“…Cornin is an iridoid glycoside derived from the fruit of Verbena officinalis L. Previous studies confirmed that cornin has inhibition effects in lipid peroxidation [15], cardioprotection against myocardial ischemic injury [16], and brain protection against cerebral ischemic injury [17,18]. However, the underlying molecular mechanism of brain protective effect during cerebral ischemia and reperfusion is unclear.…”