A dose-response curve for biodosimetry from a 6 MV electron linear accelerator

Lemos-Pinto, M. M. P.; Cadena, Marília Barbosa; Santos, Nilcilene Nascimento dos; Fernandes, T. S.; Borges, Edvane; Amaral, Ademir

doi:10.1590/1414-431x20154470

Cited by 15 publications

(17 citation statements)

References 21 publications

(23 reference statements)

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“…The overdispersion of data could be caused by nonhomogeneous irradiation of blood samples. Lemos-Pinto et al [15] stated that the overdispersion can be caused by nonhomogeneous irradiation of blood samples, resulting in nonuniformity of radiation effects, and this phenomenon was uncommon for high doses but has been reported elsewhere. [116] In this study, the dose-response curve produced here showed a significance for the highest dose at 4 Gy.…”

Section: Discussionmentioning

confidence: 99%

“…A dose-response calibration curve should be constructed by considering the basic physical parameters such as types of ionizing radiation – low- or high-LET and radiation dose rate. [15] Other factors that must be considered are dose detection limit and time interval. [20] The result may depend on the exposure conditions such as heterogeneous (partial-body) or homogeneous (whole-body) exposures, exposure time, and affected organs and organ systems.…”

Section: Discussionmentioning

confidence: 99%

“…[23] This might be due to the longer time required to irradiate the samples allowing time for DNA repair. [15] It may be important for a biodosimetry laboratory to generate several curves to cover all the types of radiations that are involved in accident scenarios. This curve may also be useful for in vitro dose reconstruction after accidental occupational exposures involving high energy X-rays, which is the basic recommendation in radiological emergency programs.…”

Section: Discussionmentioning

confidence: 99%

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Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

et al. 2019

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Chromosome aberration is a biomarker that has been used as a standard tool in biological dosimetry (biodosimetry) of individuals after exposure to ionizing radiation. It is based mainly on the induction of dicentric chromosomes – one of the radiation-induced biological effects, in order to correlate them with radiation dose. In this study, a dose calibration curve for X-rays was generated by using the dicentric assay and by fitting the data to both Chromosomal Aberration Calculation Software and Dose Estimate programs to compare the output of each method. Peripheral blood samples from four nonsmoker healthy donors were irradiated with various doses ranging from 0 to 4 Gy with 250 kV or 122 keV X-rays at a dose rate of 0.17 Gy/min. The irradiated blood was cultured, harvested, and analyzed according to the standard procedure as described by the International Atomic Energy Agency with slight modifications. The dose-response calibration data for dicentrics were fitted with the linear-quadratic model (Y dic = 0.03987D 2 + 0.00651D). The dose-response calibration curve obtained in this research was comparable to other estimations with similar radiation quality and dose rates. The results in this research convinced us in sustaining a biodosimetry using a dose-response calibration curve in our laboratory.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

et al. 2019

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“…Research conducted by Lemos-Pinto et al [21] showed that the yield of dicentrics increased with radiation dose of 6 MV electron linear accelerator. These dose-dependent results clearly indicated that the data well represented by the linear-quadratic model based on CABAS and Dose Estimate software programs.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Ionizing Radiation Induced Dicentric Chromosome and Micronuclei in Human Peripheral Blood Lymphocytes for Preliminary Reconstruction of Cytogenetic Biodosimetry

et al. 2017

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Cytological biodosimetry methodology has been widely used for determining and estimating the precise irradiation dose received by victims in the situation of emergency irradiation exposure. The aim of this study was to assess the gamma-ray induced dicentric chromosomes and micronuclei (MN) in peripheral blood lymphocytes for preliminary reconstruction of cytogenetic biodosimetry. The study was performed by exposing blood samples taken from seven healthy donors to gamma rays at dose range of 0.1 to 4.0 Gy, followed by culturing them for 48-72 hours at 37 °C by the standard technique. After being harvested, the chromosome spread at metaphase and MN were stained with Giemsa's solution. The results showed that the frequency of both dicentrics and MN of samples were increased with the increase of radiation dose. Considerable increases of both cytologic damages were found in the samples exposed to higher doses (>2 Gy). Significant differences (p>0.05) only found in mean frequencies of MN for all doses tested. Reconstruction of the relationship of these frequencies with doses was found to follow linear-quadratic curve lines and was consistent with that of other studies. Due to the aforementioned advantages namely the dependence of radiation dose and dose rate on the frequency of of both dicentric and MN, despite some limitations, these assays have been found to be suitable to be used as biological dosimetry. It is concluded that in order for this cytogenetic biodosimety method by means of scoring/assessing the radiation-induced dicentrics and MN could be used in radiation emergency and protection, and further studies with larger numbers of samples need to be done.

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“…Disponível em: https://portalperiodicos.unoesc.edu.br/archives on radiation quality (or LET-Linear Energy Transfer) and relative biological effectiveness (RBE). However, in the assessment of absorbed doses from the same irradiated blood sample, interlaboratory variations are quite common.These variations arise from several reasons such as environmental conditions of each laboratory, choice of reagents, handling procedures and equipment, and the level of training for the subjective nature of microscopic identification of unstable chromosome alterations 17. Hence, the International Atomic Energy Agency recommends that each cytogenetic laboratory should generate its own dose-response curve in order to perform biodosimetry 14.…”

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confidence: 99%

A New Methodology for Diagnosis of Fanconi Anemia Based on Biological Dosimetry

Amaral

Lima²,

Silva

et al. 2019

Arch. Biosc. Health

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Fanconi Anemia (FA) is a syndrome associated with chromosomal fragility. Current laboratory tests to diagnose this disease are based on the scoring of chromosomal aberrations induced in peripheral blood lymphocytes by clastogenic chemical agents, mainly: diepoxybutane (DEB) or mitomycin C (MMC). This study evaluated an alternative test for the diagnosis of FA, in which ionizing radiation replaces DEB/MMC. Two groups were studied: normal and DEB-sensitive individuals. From each individual, samples of peripheral blood were irradiated using an electron linear accelerator. Following lymphocyte cultures, and slide preparation, metaphases were scored based on the same methodology for biological dosimetry, according to recommendations of the International Atomic Energy Agency. Our results emphasized a pattern of distribution of dicentrics, fragments, as well as abnormal chromosomal arrangements. The methodology of analysis here proposed permitted to distinguish normal from DEB-sensitive subjects.

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A dose-response curve for biodosimetry from a 6 MV electron linear accelerator

Cited by 15 publications

References 21 publications

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

Development of Dose-Response Calibration Curve for Dicentric Chromosome Induced by X-Rays

Assessment of Ionizing Radiation Induced Dicentric Chromosome and Micronuclei in Human Peripheral Blood Lymphocytes for Preliminary Reconstruction of Cytogenetic Biodosimetry

A New Methodology for Diagnosis of Fanconi Anemia Based on Biological Dosimetry

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