The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells

Chen, W.L.; Luo, Dong-feng; Gao, Chao; Ding, Yi; Wang, S.Y.

doi:10.1590/1414-431x20154430

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Moreover, miR-181b, which is located in the intron of the FAMLF (familial acute myelogenous leukemia related factor) gene, showed an inverse correlation with FAMLF expression, and an interaction with its 5′UTR. Downregulation of FAMLF by miR-181b inhibited cell viability and arrested cell cycle in Raji BL cells [ 138 , 139 ].…”

Section: Lymphomasmentioning

confidence: 99%

MiRNA Dysregulation in Childhood Hematological Cancer

Oliveira

Roberto

Baroni

et al. 2018

IJMS

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For decades, cancer biology focused largely on the protein-encoding genes that have clear roles in tumor development or progression: cell-cycle control, apoptotic evasion, genome instability, drug resistance, or signaling pathways that stimulate growth, angiogenesis, or metastasis. MicroRNAs (miRNAs), however, represent one of the more abundant classes of cell modulators in multicellular organisms and largely contribute to regulating gene expression. Many of the ~2500 miRNAs discovered to date in humans regulate vital biological processes, and their aberrant expression results in pathological and malignant outcomes. In this review, we highlight what has been learned about the roles of miRNAs in some of the most common human pediatric leukemias and lymphomas, along with their value as diagnostic/prognostic factors.

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Section: Lymphomasmentioning

confidence: 99%

MiRNA Dysregulation in Childhood Hematological Cancer

Oliveira

Roberto

Baroni

et al. 2018

IJMS

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“…Studies have shown that FAMLF was highly expressed in acute myeloid leukemia and BL patients, NB4 acute promyelocytic leukemia cells, U937 macrophage-like cells, K562 myeloid leukemia cells, U266 myeloma cells, HL60 promyelocytic cells, CA46 lymphoma cells, and especially in Raji BL cells, while low expression was observed in unaffected individuals. FAMLF may be involved in hematopoietic neoplasms by promoting cell proliferation and preventing cell differentiation ( 6 , 7 ).…”

Section: Introductionmentioning

confidence: 99%

FAMLF is a target of miR-181b in Burkitt lymphoma

Ding

Huang

et al. 2017

Braz J Med Biol Res

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Burkitt lymphoma (BL) is a highly malignant non-Hodgkin's lymphoma that is closely related to the abnormal expression of genes. Familial acute myelogenous leukemia related factor (FAMLF; GenBank accession No. EF413001.1) is a novel gene that was cloned by our research group, and miR-181b is located in the intron of the FAMLF gene. To verify the role of miR-181b and FAMLF in BL, RNAhybrid software was used to predict target site of miR-181b on FAMLF and real-time quantitative PCR (RQ-PCR) was used to detect expression of miR-181b and FAMLF in BL patients, Raji cells and unaffected individuals. miR-181b was then transfected into Raji and CA46 cell lines and FAMLF expression was examined by RQ-PCR and western blotting. Further, Raji cells viability and proliferation were detected by MTT and clone formation, and Raji cell cycle and apoptosis were detected by flow cytometry. The results showed that miR-181b can bind to bases 21–42 of the FAMLF 5′ untranslated region (UTR), FAMLF was highly expressed and miR-181b was lowly expressed in BL patients compared with unaffected individuals. FAMLF expression was significantly and inversely correlated to miR-181b expression, and miR-181b negatively regulated FAMLF at posttranscriptional and translational levels. A dual-luciferase reporter gene assay identified that the 5′ UTR of FAMLF mRNA contained putative binding sites for miR-181b. Down-regulation of FAMLF by miR-181b arrested cell cycle, inhibited cell viability and proliferation in a BL cell line model. Our findings explain a new mechanism of BL pathogenesis and may also have implications in the therapy of FAMLF-overexpressing BL.

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The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells

Cited by 2 publications

References 22 publications

MiRNA Dysregulation in Childhood Hematological Cancer

MiRNA Dysregulation in Childhood Hematological Cancer

FAMLF is a target of miR-181b in Burkitt lymphoma

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