Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats

Tavares, José Roberto; Vasques, Ênio Rodrigues; Arida, Ricardo Mário; Cavalheiro, Ésper A.; Cabral, Francisco Romero; Torres, Laila Brito; Rodrigues, Francisco Sandro Menezes; Jurkiewicz, Aron; Caricati‐Neto, Afonso; Godoy, Carlos; Silva, Sérgio Gomes da

doi:10.1590/1414-431x20144311

Cited by 12 publications

(19 citation statements)

References 32 publications

(36 reference statements)

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“…This Ca 2+ overload increases Ca 2+ influx in mitochondrial matrix, promoting mitochondrial Ca 2+ overload and ATP production [8]. These cellular alterations caused by myocardial I/R severely compromise cardiac excitation-contraction coupling, contributing to development of cardiac arrhythmias and death in animals [7,8,13,15] and AMI patients [9].…”

Section: Discussionmentioning

confidence: 99%

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A Simple and Efficient Methodology for the Study of Cardioprotective Drugs in Animal Model of Cardiac Ischemia-Reperfusion

Tavares¹,

Francisco²,

Menezes-Rodrigues³

et al. 2017

J Mol Imaging Dynam

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To study cardioprotective drugs, we developed a simple and efficient methodology to evaluate effects of drugs on cardiac electrical activity using electrocardiogram (ECG) in rats submitted to cardiac ischemia-reperfusion (I/R). Using adult male Wistar rats (14 -16-week-old) anesthetized (urethane 1.25 g/kg, i.p.) and kept under mechanical ventilation, we used surgical procedures to induce cardiac I/R by means mechanical occlusion of left anterior descendent coronary artery for 10 min (ischemia) with silk suture (tourniquet) followed by its removal to allow coronary recirculation (reperfusion). To evaluate the effects of surgical process, a group of rats (SHAM-operated) was submitted to surgical procedures previously described, but without coronary occlusion. To evaluate cardiac electrical activity in rats submitted to cardiac I/R and SHAM-operated, the ECG system was coupled to animal body to determine the incidence of ventricular arrhythmia (VA), atrio-ventricular blockade (AVB) and lethality (LET). To evaluate injury biomarkers production in rats submitted to cardiac I/R and SHAM-operated, serum concentrations of creatine kinase fraction (MB/CK-MB) and troponin I were determined by biochemical techniques. Using the methodology proposed in this work, we observed that VA, AVB and LET incidence was significantly higher in cardiac I/R group (85%, 79% and 70%, respectively) than in SHAM-operated group (0%, 0% and 0%, respectively). Serum levels of CK-MB and troponin I were also significantly higher in cardiac I/R (1,850 ± 222 U/L and 0.031 ± 0.009 ng/mL, respectively) compared to SHAM-operated group (808 ± 72 U/L and 0.200 ± 0.027 ng/mL). To evaluate efficiency of methodology proposed in this work to study the effect of cardioprotective drugs, the effects of the L-type Ca 2+ channel blocker (CCB) nifedipine (30 mg/kg, intravenously -IV) in rats submitted to cardiac I/R were studied. The treatment with nifedipine (before ischemia) significantly reduced the incidence of VA (from 85% to 28%), AVB (from 79% to 14%), and LET (from 70% to 14%). These results indicate that the methodology described in the present work is simple, and efficient, to evaluate cardiac functional and biochemical alterations induced by cardiac I/R, and also the study of cardioprotective drugs in rats. This methodology could contribute to the development of new pharmacological cardioprotective strategies for to treatment of ischemic cardiac diseases in humans, such as myocardial infarction.

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Section: Discussionmentioning

confidence: 99%

“…In the present work, it was used a similar method to induce cardiac I/R previously described in other study published by our lab [13]. All rats were anesthetized with urethane (1.25 g/kg), and fixed in the supine position.…”

Section: Protocol Of Cardiac Ischemia and Reperfusion (I/r)mentioning

confidence: 99%

A Simple and Efficient Methodology for the Study of Cardioprotective Drugs in Animal Model of Cardiac Ischemia-Reperfusion

Tavares¹,

Francisco²,

Menezes-Rodrigues³

et al. 2017

J Mol Imaging Dynam

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“…In a recent study, the incidence of ischemia/ reperfusion (I/R)-induced ventricular arrhythmias was found to be higher in isolated hearts from rats which had been affected by audiogenic epileptic seizure (10). In contrast with this finding, Tavares et al reported that pilocarpine-induced epilepsy did not affect the incidence of ventricular arrhythmias in anesthetized rats (11).…”

Section: Introductionmentioning

confidence: 92%

The effect of generalized seizure activity on ischemia-induced cardiac arrhythmias and myocardial injury with histopathological evaluation in anesthetized rats

2018

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Background/aim: Epileptic seizure leads to sudden unexpected death in epilepsy (SUDEP) among affected patients. The causes of SUDEP are still unclear. The aim of this study was to research the effect of epilepsy on myocardial injury and arrhythmias during experimentally induced acute myocardial ischemia. Materials and methods: Wistar albino rats were divided into four groups: sham, pentylentetrazole (PTZ) + sham, ischemia, and PTZ + ischemia groups. PTZ (65 mg/kg, ip) was given 2 h before ischemia. Seizure scoring was conducted by evaluating the PTZ-induced behavioral changes in the rats. The left main coronary artery was ligated in anesthetized rats for 30 min. The incidence and the number of ventricular arrhythmias were determined. Histopathological scoring was performed for tissue injury by using a microscope. Results: Seizure scores were not different among the groups (P > 0.05). The incidence and number of ventricular tachycardia (VT) episodes were significantly higher in the PTZ + ischemia group than in the ischemia group (P ˂ 0.05). More prominent myocardial damage was observed in the PTZ + ischemia group than in the other groups (histopathological scores: PTZ + ischemia; 2.5 ± 0.5 versus ischemia; 1.2 ± 0.4, P ˂ 0.05). Conclusion: PTZ-induced seizure in rats increased myocardial injury and the incidence and number of VT episodes in myocardial ischemia. These results reveal that seizure in epilepsy patients may increase ventricular arrhythmia and myocardial injury during heart attack.

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“…Several drugs are used for the pharmacotherapy of obesity-FDA approved pharmacological monotherapy options-including orlistat (ORL, pancreatic lipase inhibitor) [12,13]. Therefore, our group decided to evaluate potential cardioprotective effects of the agents used in the pharmacotherapy (such as ORL) of dyslipidemia in normotensive ratstreated with ORL for ten days-and submitted to the model of in vivo cardiac I/R developed by our group [14]. The cardioprotection was analyzed by evaluation of the electrophysiological parameters through the electrocardiogram analysis (arrhythmias), and serum concentration biochemical markers of cardiac lesion produced in response to the cardiac I/R protocol (creatine kinase (CK)), lowdensity lipoprotein cholesterol (LDL-C) and lethality.…”

Section: Editorialmentioning

confidence: 99%

Potential Cardioprotective Effects of Orlistat for Treatment of Myocardial Infarction

Rodrigues¹,

Tavares²,

Araújo³

et al. 2017

J Thrombo Cir

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Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats

Cited by 12 publications

References 32 publications

A Simple and Efficient Methodology for the Study of Cardioprotective Drugs in Animal Model of Cardiac Ischemia-Reperfusion

A Simple and Efficient Methodology for the Study of Cardioprotective Drugs in Animal Model of Cardiac Ischemia-Reperfusion

The effect of generalized seizure activity on ischemia-induced cardiac arrhythmias and myocardial injury with histopathological evaluation in anesthetized rats

Potential Cardioprotective Effects of Orlistat for Treatment of Myocardial Infarction

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