Therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia using Fe2O3 nanoparticles

Abstract

This study aimed to investigate the therapeutic mechanism of treating SMMC-7721 liver cancer cells with magnetic fluid hyperthermia (MFH) using Fe2O3 nanoparticles. Hepatocarcinoma SMMC-7721 cells cultured in vitro were treated with ferrofluid containing Fe2O3 nanoparticles and irradiated with an alternating radio frequency magnetic field. The influence of the treatment on the cells was examined by inverted microscopy, MTT and flow cytometry. To study the therapeutic mechanism of the Fe2O3 MFH, Hsp70, Bax, Bcl-2 and p53 were detected by immunocytochemistry and reverse transcription polymerase chain reaction (RT-PCR). It was shown that Fe2O3 MFH could cause cellular necrosis, induce cellular apoptosis, and significantly inhibit cellular growth, all of which appeared to be dependent on the concentration of the Fe2O3 nanoparticles. Immunocytochemistry results showed that MFH could induce high expression of Hsp70 and Bax, decrease the expression of mutant p53, and had little effect on Bcl-2. RT-PCR indicated that Hsp70 expression was high in the early stage of MFH (<24 h) and became low or absent after 24 h of MFH treatment. It can be concluded that Fe2O3 MFH significantly inhibited the proliferation of in vitro cultured liver cancer cells (SMMC-7721), induced cell apoptosis and arrested the cell cycle at the G2/M phase. Fe2O3 MFH can induce high Hsp70 expression at an early stage, enhance the expression of Bax, and decrease the expression of mutant p53, which promotes the apoptosis of tumor cells.

Efficacy of hyperthermia in human colon adenocarcinoma cells is improved by auraptene

“…Analysis of cell cycle revealed increased presence of HT29 cells in G 2 /M phase after heat shock application, while combination of hyperthermia with auraptene resulted in considerable accumulation of cells in sub G 1 /G 1 phase. In consistence with our findings, G 2 /M phase arrest was reported after hyperthermia in several cell types such as liver, lung, breast and renal cancer cells (Qi et al 2015;Zhao et al 2015;Yan et al 2014;Lin et al 2013). Same as present results, previous studies have also indicated growth controlling effects of auraptene on breast and gastric carcinoma cells by cell cycle arrest in sub G 1 (Mousavi et al 2015, Moon et al 2015 and G 0 -G 1 phases (de Medina et al 2010;Krishnan et al 2009).…”

A Novel Model of Cancer

“…Reports display that Fe 2 O 3 in nanoparticles had a therapeutic effect on cancer cells in vitro and xenograft liver cancer in mice and where the result was increased inhibitory rate and ratio of apoptosis [11]. Another report describes the therapeutic mechanism of treating SMMC-7721 liver cancer cells using Fe 2 O 3 nanoparticles.…”

“…Several studies have shown that a history of autoimmune diseases increases the risk of cancer [8], despite the fact that cancer and autoimmune diseases are fundamentally different pathological conditions [9]. Numerous reports display that the molecule Fe 2 O 3 increases cellular necrosis, increases the rate of apoptosis [10] and significantly inhibits cellular growth [11]. Fe 2 O 3 is the active substance in Ferumoxytol [12].…”

Skin Cancer and Its Treatment: Novel Treatment Approaches with Emphasis on Nanotechnology

“…Their surface can be augmented with various functional groups, increasing biocompatibility and biodegradability, which further increases their usefulness. Polymers like cellulose, dextran, PEG, or PLGA that can also be added to the surface increase their biocompatibility and biodegradability [153][154][155].…”