Antioxidant effect of 4-nerolidylcatechol and α-tocopherol in erythrocyte ghost membranes and phospholipid bilayers

Abstract

4-Nerolidylcatechol (4-NC) is found in Pothomorphe umbellata root extracts and is reported to have a topical protective effect against UVB radiation-induced skin damage, toxicity in melanoma cell lines, and antimalarial activity. We report a comparative study of the antioxidant activity of 4-NC and α-tocopherol against lipid peroxidation initiated by two free radical-generating systems: 2,2′-azobis(2-aminopropane) hydrochloride (AAPH) and FeSO4/H2O2, in red blood cell ghost membranes and in egg phosphatidylcholine (PC) vesicles. Lipid peroxidation was monitored by membrane fluidity changes assessed by electron paramagnetic resonance spectroscopy of a spin-labeled lipid and by the formation of thiobarbituric acid-reactive substances. When lipoperoxidation was initiated by the hydroxyl radical in erythrocyte ghost membranes, both 4-NC and α-tocopherol acted in a very efficient manner. However, lower activities were observed when lipoperoxidation was initiated by the peroxyl radical; and, in this case, the protective effect of α-tocopherol was lower than that of 4-NC. In egg PC vesicles, malondialdehyde formation indicated that 4-NC was effective against lipoperoxidation initiated by both AAPH and FeSO4/H2O2, whereas α-tocopherol was less efficient in protecting against lipoperoxidation by AAPH, and behaved as a pro-oxidant for FeSO4/H2O2. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical assay indicated that two free radicals were scavenged per 4-NC molecule, and one free radical was scavenged per α-tocopherol molecule. These data provide new insights into the antioxidant capacity of 4-NC, which may have therapeutic applications for formulations designed to protect the skin from sunlight irradiation.

O Uso De Plantas Medicinais Pela População De União Da Vitória – Pr: O Saber Popular Confrontado Pelo Conhecimento Científico

“…Usado para pressão alta, descrito como antioxidante (Fernandes et al, 2013) e antitumoral (Sacoman et al, 2008)…”

Correction of the Hematological Homeostasis Parameters during Physical Loads by Using Energy Orientation Pharmacological Agents

“…У контрольній групі спортсменів співвідношення МДА і GSH наприкінці досліджень перевищувало 4,5 ум. од., що в 1,67 рази більше порівняно з даними до початку дослідження і свідчить про пошкодження структури ліпідного шару біологічних мембран [20] за рахунок змін ПАР факторів у бік накопичення прооксидантних факторів при одночасному зниженні вмісту неферментативних антиоксидантних і наступного формування окисного стресу [27].…”

Theoretical Investigation of the Structural, Spectroscopic, Electronic, and Pharmacological Properties of 4-Nerolidylcathecol, an Important Bioactive Molecule

“…Its potent antioxidant activity is largely established in the literature [8][9][10][11][12], but other pharmacological properties, such as antimicrobial [13], analgesic, anti-inflammatory [14], cytotoxic [15,16], and antimalarial activities [17] are related to 4NRC. It is the major secondary metabolite of Piper genus plants, traditionally known for their use in the treatment of various diseases [12,15,[17][18][19][20]. However, the therapeutic applicability of 4NRC is limited by its very poor water solubility.…”

“…Many works in the literature have discussed the pharmacological properties [10,12,16,[18][19][20][21][22] of 4NRC, but studies on the chemical-quantum, spectroscopic, and structural properties of 4NRC are still lacking.…”