A manual method to obtain platelet rich plasma

Marques, Fabiana Paulino; Ingham, Sheila Jean McNeill; Forgas, Andrea; Franciozi, Carlos Eduardo da Silveira; Sasaki, Pedro Henrique; Abdalla, René Jorge

doi:10.1590/1413-78522014220200798

Cited by 7 publications

(5 citation statements)

References 22 publications

(22 reference statements)

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“…The gel is injected to the desirable site. It should be noted that there is a difference in platelet quantity: platelet and WBC count is higher in younger people and higher in females compared to males [15,21,22]. [16,23].…”

Section: The Techniquementioning

confidence: 99%

Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Mijiritsky

Assaf

Peleg

et al. 2021

Biology

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Growth factors (GFs) play a vital role in cell proliferation, migration, differentiation and angiogenesis. Autologous platelet concentrates (APCs) which contain high levels of GFs make them especially suitable for periodontal regeneration and facial rejuvenation. The main generations of APCs presented are platelet-rich plasma (PRP), platelet-rich fibrin (PRF) and concentrated growth factor (CGF) techniques. The purpose of this review is to provide the clinician with an overview of APCs’ evolution over the past decade in order to give reliable and useful information to be used in clinical work. This review summarizes the most interesting and novel articles published between 1997 and 2020. Electronic and manual searches were conducted in the following databases: Pubmed, Scopus, Cochrane Library and Embase. The following keywords were used: growth factors, VEGF, TGF-b1, PRP, PRF, CGF and periodontal regeneration and/or facial rejuvenation. A total of 73 articles were finally included. The review then addresses the uses of the three different techniques in the two disciplines, as well as the advantages and limitations of each technique. Overall, PRP is mainly used in cases of hard and soft tissue procedures, while PRF is used in gingival recession and the treatment of furcation and intrabony defects; CGF is mainly used in bone regeneration.

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Section: The Techniquementioning

confidence: 99%

Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Mijiritsky

Assaf

Peleg

et al. 2021

Biology

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“…Another study proved that centrifugation of 541 g for 61 mL of whole blood at 5 minutes was optimal for obtaining high platelet in the sample. 14 In this study, we also included a single centrifugation technique that is commonly used. The platelet counts in PRP can affect and positively correlate with the concentration of growth factors and better clinical results.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality

Maharani¹,

Astuti²

2022

AMJ

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Background: Platelet-rich plasma (PRP) is a new concept used in medical world, especially for wound healing. The main process that affects the PRP quality is the centrifugation process. This study aimed to assess the PRP separation process and determine the best technique of various centrifugation process.Methods: This experimental study used acid citrate dextrose (ACD) blood taken from 11 healthy respondents and compared three-techniques including the single centrifugation (SC), the double centrifugation (DC), and the double centrifugation turn down - turn up (DC-TDTU) techniques. The quality of PRP was measured based on blood cells count (platelet, leukocyte, erythrocyte count, and Ht value) at each stage of centrifugation. The examination was carried out in 2021 at the Hematology Laboratory, Poltekkes Jakarta 3.Results: The mean values of platelets, leukocytes, and Ht were increased in PRP compared to plasma supernatant both using the DC and DC-TDTU techniques, wherase the SC technique decreased in plasma compared with whole blood. When the procedures using DC and DC-TDTU are carried out properly, platelets would be concentrated in the second centrifugation. However, some erythrocyte and leukocyte contamination occurred by DC-TDTU technique compared to the DC technique.Conclusion: The double centrifugation technique is the best Platelet-rich plasma separation technique compared to the DC-TDTU and SC techniques.

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“…It could be argued that the age and gender have some effect on PRP. For instance, studying 637 people, Marques et al found that platelet count in women and young people were higher than in men and older people ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of the application of platelet rich plasma in sports medicine

Gholami¹,

Ravaghi²,

Salehi³

et al. 2016

Electron physician

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IntroductionIn recent years, platelet rich plasma (PRP) has been receiving increasing attention for the treatment of soft tissue injuries. These numerous applications have raised a great deal of questions and debate about the effectiveness of this method. This study aimed to determine the efficacy of PRP in improving sports injuries and subsequently throw some light on these controversies.MethodsA systematic review of the literature and meta-analysis of results were undertaken. All related databases, such as PubMed, Cochrane Database of Systematic Reviews, DARE, and EMBASE, were searched on the use of PRP on athletes and in sports medicine. The search was conducted from June 2013 to February 2014.ResultsOur search retrieved 905 studies, of which 13 randomized control trials (RCT) met our inclusion criteria for systematic review and meta-analysis. All articles were appraised by Critical Appraisal Skills Program (CASP) checklist for RCT studies. The analysis of the results of pain scores and physical activity/functions did not show any superiority for PRP as opposed to the other options.ConclusionsThe meta-analysis showed no more effectiveness for PRP application in sports-related injuries in terms of physical function improvement and pain relief. Therefore, the extensive use of PRP for such injuries should be limited. Well-designed RCTs are needed to support the findings.

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A manual method to obtain platelet rich plasma

Cited by 7 publications

References 22 publications

Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Use of PRP, PRF and CGF in Periodontal Regeneration and Facial Rejuvenation—A Narrative Review

Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality

A systematic review and meta-analysis of the application of platelet rich plasma in sports medicine

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