ATIVIDADE ANTIBACTERIANA IN VITRO DO EXTRATO DE Abarema cochliacarpos (GOMES) BARNEBY & J.W. GRIMES CONTRA BACTÉRIAS ISOLADAS DE FERIDAS CUTÂNEAS DE CÃES

Abstract

ResumoAbarema cochliacarpos é uma espécie nativa do Brasil, pertence à família Leguminosae -Mimosoidae, muito utilizada na medicina popular. Objetivou-se avaliar a atividade antibacteriana in vitro dos extratos ciclohexânico, acetônico e etanólico da casca de Abarema cochliacarpos (Gomes) Barneby & J.W. Grimes contra bactérias isoladas de feridas cutâneas de cães. A atividade antibacteriana dos extratos foi determinada pelo método de difusão em meio sólido enquanto que a Concentração Mínima Inibitória foi determinada em microplacas. Alíquotas dos poços sem crescimento bacteriano após 24h de incubação a 35-37 o C foram adicionadas em placas com meio de cultura para determinação da Concentração Mínima Bactericida. A análise fitoquímica da planta revelou resultados positivos para alcalóides, flavonóides, saponinas, taninos (condensáveis), terpenos e esteróides. As bactérias isoladas foram: Staphylococcus intermedius, Bacillus sp., Pasteurella sp. e Escherichia coli. Os ensaios in vitro dos extratos da casca de Abarema cochliacarpos mostraram inibição contra as bactérias Gram-positivas Staphylococcus intermedius e Bacillus sp. nas concentrações testadas (100, 50, 25, 12,5 e 6,25 mg/mL), exceto para o extrato em ciclohexano que não demonstrou inibição nas concentrações de 12,5 e 6,25 mg/mL contra o Staphylococcus intermedius. Não houve halos de inibição frente aos isolados Gram-negativos Pasteurella sp. e Escherichia coli. Concluiu-se que os extratos ciclohexânico, acetônico e etanólico da casca de Abarema cochliacarpos mostraram atividade antibacteriana, para a maioria das concentrações testadas, frente às cepas Gram-positivas Staphylococcus intermedius e Bacillus sp. isoladas de feridas cutâneas de cão. Palavras-chave: ação antibacteriana; barbatimão; fitoquímica; Mimosoidae; taninos. AbstractAbarema cochliacarpos is a native species of Brazil, it belongs to the family Leguminosae -Mimosoidae, and it has been long used in folk medicine. This study aimed to evaluate the in vitro antibacterial activity of cyclohexane, acetone, and ethanol extracts of the bark of Abarema cochliacarpos (Gomes) Barneby & JW Grimes against bacteria isolated from skin wounds in dogs. The antibacterial activity of the extracts was determined by the diffusion method on solid medium while the Minimal Inhibitory Concentration was determined in microplates. Aliquots of the wells without bacterial growth after 24 hours of incubation at 35-37 o C were added to plates with culture medium to determine Minimum Bactericidal Concentration. The phytochemical analysis of the plant showed positive results for

Antioxidant and cicatrizing activity of the species Abarema cochliacarpos (Gomes) Barneby J. W. Grimes

“…In addition, A. cochliacarpos has pharmacological potential against Gram-positive bacteria, mainly of the genus Staphylococcus, and can be exploited in the future to obtain bioactive compounds with antibacterial action (Tenório et al, 2016). The hydroethanolic extract from stem barks of A. cochliacarpos for the treatment of rats with induced burns and infected with strains of S. aureus reported that the animals showed complete healing in 21 days (Soares et al, 2013).…”

“…Many traditional communities in northeast Brazil use its bark in folk medicine (Agra et al, 2008;Pereia et al, 2013). An ethnopharmacological survey in a Brazilian traditional community has documented popular uses of this species to treat inflammatory disorders, including ulcerations, and for wound healing purposes (Tenório et al, 2016).…”

HPLC/HR‐MS‐Based Metabolite Profiling and Chemometrics: A Powerful Approach to Identify Bioactive Compounds from Abarema cochliacarpos

“…Abarema cochliacarpos (Gomes) Barneby & J.W. Grimes is a Brazilian native species that belongs to the Leguminosae family, which occurs mainly at the Atlantic Forest, Caatinga and rupestrian field biomes [1] . A. cochliacarpos bark is used in the traditional medicine to treat leucorrhea, uterine bleeding, inflammations, purulent wounds, vaginal irritation, and gastric ulcers.…”

“…A. cochliacarpos bark is used in the traditional medicine to treat leucorrhea, uterine bleeding, inflammations, purulent wounds, vaginal irritation, and gastric ulcers. Additionally, A. cochliacarpos extracts possess a diverse range of biological activities such as antibacterial, [1] anti‐inflammatory, [2–5] antioxidant, [6,7] gastroprotective and ulcer‐healing [8] …”

“…Phytochemical screening assays are preliminary qualitative tests that indicate the presence or absence of a specific class of metabolites in a given extract by means of a series of colorimetric and precipitation reactions [10,11] . Phytochemical screening assays of A. cochliacarpos extracts revealed the presence of alkaloids, flavonoids, saponins, tannins (condensable), terpenoids, leucoanthocyanidins and steroids [1,6] . However, there is a lack of in‐depth large‐scale metabolite profiling studies of A. cochliacarpos .…”