SIMULTANEOUS LIPID AND CAROTENOID PRODUCTION BY STEPWISE FED-BATCH CULTIVATION OF Rhodotorula mucilaginosa WITH CRUDE GLYCEROL

Abstract

In the present study, an innovative cultivation strategy of Rhodotorula mucilaginosa CCT 7688 to accumulate both lipids and high added value carotenoids was proposed, with the potential to contribute to the economic feasibility of microbial oils in comparison with vegetable oils. The stepwise fed-batch cultivation with crude glycerol feeding at 24 h, 96 h, and 144 h, with the addition of magnesium to the initial culture medium, resulted in a lipid content of 51.0 ± 0.3 % CDW, biomass concentration of 21.00 ± 0.48 g L-1 , total lipid production of 10.72 ± 0.18 g L-1 , lipid productivity of 0.037 ± 0.001 g L-1 h-1 , volumetric carotenoid production of 2843.2 ± 282.0 μg L-1 , and carotenoid productivity of 9.87 ± 0.98 μg L-1 h-1. These values represent a 6-fold increase in lipid content, a 19-fold increase in lipid production, and a 2-fold increase in carotenoid production compared to batch cultivation.

Coproduction of Microbial Oil and Carotenoids within the Circular Bioeconomy Concept: A Sequential Solid-State and Submerged Fermentation Approach

“…where X (R i ) was the obtained value at every level of each factor studied, min X (R i ) represents a value lower than the minimum obtained in the whole set of data and max X (R i ) , values for each response were based on the maximum production of carotenoids and lipids reported in the literature [20,[43][44][45]. The global desirability function was defined as:…”

“…Oleaginous yeasts have been exploited recently as plausible cell factories due to their biotechnological capacity to produce a variety of added-value compounds [10,12,13] and their main advantages such as short periods of cellular growth, strain capacity to use a wide variety of substrates, no land utilization and feasibility of process scale-up [14][15][16]. The mostreported biomolecules synthesized by oleaginous yeast are lipids and carotenoids [17][18][19][20], and its concomitant production in the genus related to Rhodotorula, Rhodosporidium, and Sporobolomyces is possible due to the common precursor acetyl-coA in lipogenesis and carotenogenesis [21,22]. Remarkably, oleaginous yeast that can accumulate carotenoids become an extra value to bioprocess.…”

Current Advances in Carotenoid Production by Rhodotorula sp.

“…Therefore, the fed-batch mode can reliably provide nutritional supplements during the stationary phase [50]. For example, studies carried out on R. mucilaginosa demonstrated that fed-batch cultivation feeding with crude glycerol achieved a 2-fold increase in carotenoid production compared to batch culture [87]. Rodrigues et al [35] reported that carotenoid production from R. mucilaginosa grown in an agro-industrial environment also had a 12-fold increase in carotenoid production in a fed-batch fermentation setup compared to batch fermentation.…”

Bioremediation with an Alkali-Tolerant Yeast of Wastewater (Nejayote) Derived from the Nixtamalization of Maize

“…It generates mucoid colonies in various tones of pinkish orange, a color given by its production of carotenoids such as β-carotene, torulene, and torularhodin [64][65][66]. It has the capacity to assimilate different sources of carbon, including glucose, sucrose, and fructose, and to grow in complex substrates (e.g., molasses, whey, ketchup, and raw glycerol) [64,[66][67][68].…”