ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

Rodrigues, Plínio Ribeiro; Nunes, Jéssica Magalhães Neves; Lordelo, Luciana N.; Druzian, Janice Izabel

doi:10.1590/0104-6632.20190361s20170267

Cited by 13 publications

(5 citation statements)

References 44 publications

(59 reference statements)

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“…The degree of crystallinity (X c ) is one of the most important characteristics among the different mechanical performance and processability properties of the polymer and is summarized in Table 5. To minimize polymer processing challenges, X c should ideally be ≤ 50%; otherwise, corrective actions are required resulting in challenges during polymer processing that can increase the operation price (Rodrigues et al 2019). It was observed that the X c for the cyclic events decreased from 52% at CFBF 1 to 34% after CFBF 4 (Table 5).…”

Section: Dsc Analysismentioning

confidence: 99%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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The accumulation of petrochemical plastic waste is detrimental to the environment. Polyhydroxyalkanoates (PHAs) are bacterial-derived polymers utilized for the production of bioplastics. PHA-plastics exhibit mechanical and thermal properties similar to conventional plastics. However, high production cost and obtaining high PHA yield and productivity impedes the widespread use of bioplastics. This study demonstrates the concept of cyclic fed-batch fermentation (CFBF) for enhanced PHA productivity by Bacillus thuringiensis using a glucose-rich hydrolyzate as the sole carbon source. The statistically optimized fermentation conditions used to obtain high cell density biomass (OD600 of 2.4175) were: 8.77 g L−1 yeast extract; 66.63% hydrolyzate (v/v); a fermentation pH of 7.18; and an incubation time of 27.22 h. The CFBF comprised three cycles of 29 h, 52 h, and 65 h, respectively. After the third cyclic event, cell biomass of 20.99 g L−1, PHA concentration of 14.28 g L−1, PHA yield of 68.03%, and PHA productivity of 0.219 g L−1 h−1 was achieved. This cyclic strategy yielded an almost threefold increase in biomass concentration and a fourfold increase in PHA concentration compared with batch fermentation. FTIR spectra of the extracted PHAs display prominent peaks at the wavelengths unique to PHAs. A copolymer was elucidated after the first cyclic event, whereas, after cycles CFBF 2–4, a terpolymer was noted. The PHAs obtained after CFBF cycle 3 have a slightly higher thermal stability compared with commercial PHB. The cyclic events decreased the melting temperature and degree of crystallinity of the PHAs. The approach used in this study demonstrates the possibility of coupling fermentation strategies with hydrolyzate derived from lignocellulosic waste as an alternative feedstock to obtain high cell density biomass and enhanced PHA productivity.

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Section: Dsc Analysismentioning

confidence: 99%

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Singh

Sitholé

Lekha

et al. 2021

Bioresour. Bioprocess.

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“…This result suggests that C. necator is a better PHA producer than B. cepacia from hydrolyzed BSG. Previously, Rodrigues et al [58] compared the PHA production using C. necator and B. cepacia from soybean as a substrate. In that study, C. necator resulted better than B. cepacia to produce PHA, reaching up to 0.84 g P3HB•L −1 .…”

Section: Pha Production From Sugar-rich Hydrolysatesmentioning

confidence: 99%

Brewer’s spent grain biotransformation to produce lignocellulolytic enzymes and polyhydroxyalkanoates in a two-stage valorization scheme

Llimós-Turet

Martínez-Avila

Marti

et al. 2020

Biomass Conv. Bioref.

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Lignocellulolytic enzymes from low-cost sources are gaining attention as a tool to reduce production costs. Such enzymes can be obtained sustainably by diverse fungal strains via solid-state fermentation (SSF) of lignocellulosic-derived residues as substrates. Besides, these enzymes allow hydrolyzing the same residue, releasing fermentable sugars that can be transformed into valueadded products. This study shows a two-stage valorization approach for the lignocellulosic leftover brewer's spent grain (BSG): first, by producing lignocellulolytic enzymes through the SSF of BSG using three fungal strains and, second, by using the selfproduced enzymes to hydrolyze the same BSG and obtaining sugar-rich hydrolysates that serve as an alternative carbon source for polyhydroxyalkanoates (PHA) production. From the evaluated set, Aspergillus niger and Thermoascus aurantiacus produced the highest xylanase activities compared with Trichoderma reesei (268 ± 24, 241 ± 10, and 150 ± 24 U per gram of dry BSG, respectively). Also, A. niger extracts resulted in the most effective for releasing sugars from BSG, obtaining up to 0.56 g per gram of dry BSG after 24 h without any pretreatment needed. Thus, the sugar-rich hydrolysate obtained with A. niger was used as a source for producing PHA by using two bacterial strains, namely, Burkholderia cepacia and Cupriavidus necator. Maximum PHA yield was achieved by using C. necator after 48 h with 9.0 ± 0.44 mg PHA•g −1 dry BSG. These results show the significant potential of BSG as raw material for obtaining value-added bioproducts and the importance of multiple valorization schemes to improve the feasibility of similar residue-based systems.

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“…Among the waste streams identified, there is convincing scientific evidence in support of the higher carbon content in oilbased feedstocks (Akiyama et al, 2003). So far, PHA production using waste plant oils (e.g., palm kernel oil, crude palm oil, palm oil, jatropha oil, sludge palm oil, soybean oil) as inexpensive carbon sources has been taken into consideration owing to its widespread availability and renewability (Lee et al, 2008;Sudesh et al, 2011;Rodrigues et al, 2019;Thinagaran and Sudesh, 2019). Different agriculture sectors generate different types of waste.…”

Section: Introductionmentioning

confidence: 99%

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

Surendran

Lakshmanan

Chee

et al. 2020

Front. Bioeng. Biotechnol.

101

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Polyhydroxyalkanoates (PHAs) are a potential replacement for some petrochemical-based plastics. PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions. PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources. Such fermentation conditions have been optimized by industry to reduce the cost of PHAs produced commercially. Industrially, these biodegradable polyesters are derived from microbial fermentation processes utilizing various carbon sources. One of the major constraints in scaling-up PHA production is the cost of the carbon source metabolized by the microorganisms. Hence, cheap and renewable carbon substrates are currently being investigated around the globe. Plant and animal oils have been demonstrated to be excellent carbon sources for high yield production of PHAs. Waste streams from oil mills or the used oils, which are even cheaper, are also used. This approach not only reduces the production cost for PHAs, but also makes a significant contribution toward the reduction of environmental pollution caused by the used oil. Advancements in the genetic and metabolic engineering of bacterial strains have enabled a more efficient utilization of various carbon sources, in achieving high PHA yields with specified monomer compositions. This review discusses recent developments in the biosynthesis and classification of various forms of PHAs produced using crude and waste oils from the oil palm and fish industries. The biodegradability of the PHAs produced from these oils will also be discussed.

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ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

Cited by 13 publications

References 44 publications

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Optimization of cultivation medium and cyclic fed-batch fermentation strategy for enhanced polyhydroxyalkanoate production by Bacillus thuringiensis using a glucose-rich hydrolyzate

Brewer’s spent grain biotransformation to produce lignocellulolytic enzymes and polyhydroxyalkanoates in a two-stage valorization scheme

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?

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