BIOCHEMICAL RESPONSES FROM BIOMASS OF ISOLATED Chlorella sp., UNDER DIFFERENT CULTIVATION MODES: NON-LINEAR MODELLING OF GROWTH KINETICS

Abstract

In this study, the effect of different modes of cultivation viz., photoautotrophic (AT), heterotrophic (HT) and photomixotrophic (MT), on the growth and biochemical responses of Chlorella sp. isolated from local ponds. The performance of microalgal growth was quantified using nonlinear growth models such as Gompertz, logistic, Baranyi-Roberts, Morgan under different cultivation conditions. The results revealed that microalgae could grow better in MT than in other cultivation modes with a major increase in biochemical responses for carbohydrates, which showed higher accumulation under HT. The maximum biomass concentration was 1.24 g L-1 (MT), 1.16 g L-1 (HT), 0.76 g L-1 (AT) with maximum specific growth rates of 0.0083 h-1 (HT), 0.0078 h-1 (MT) and 0.0055 h-1 (AT) respectively. The biomass concentration was higher in the order of MT>HT>AT for which MT yielded 8.8 fold higher biomass, compared with the initial biomass concentration, at the end of experiments (16 days). Concomitant increases in biochemical responses were observed in the three cultivation conditions. Protein and lipid accumulation in the MT mode was higher (1.2 fold) compared with the initial protein yield as well as the other cultivation modes. However, the carbohydrate yield was higher (1.12 fold) in the heterotrophic mode than in other cultivation conditions.

Effects of different culture media on physiological features and laboratory scale production cost of Dunaliella salina

“…Different biochemical contents were also reported for microalgal biomass cultivated under different media [ 6 , 12 , 50 ]. The biochemical comparison among microalgal biomass suggests that medium components present an intrinsic relationship in cell metabolism promoting the increase of a specific molecule; therefore, it can be further used to promote the enhancement of biocompounds of interest.…”

GROWTH, CARBOHYDRATE PRODUCTIVITY AND GROWTH KINETIC STUDY OF Halochlorella rubescens CULTIVATED UNDER CO2-RICH CONDITIONS

“…Appropriate models have been used in the study of microalgae growth and product formation in order to reduce the overall cost of growth production, particularly in the context of large-scale application (Yang et al, 2011). According to the study by Praveen et al (2018), which showed that different cultivation mode would have different growth model. Previous study by Béchet, et al (2017) described that the cultivation of Dunaliella salina was well adapted to the Weibull model under high temperature condition.…”