ETHANOL PRODUCTION FROM SUGAR LIBERATED FROM Pinus SP. AND Eucalyptus SP. BIOMASS PRETREATED BY IONIC LIQUIDS

Abstract

Pretreatment of lignocellulosic biomass using ionic liquids (ILs) has been widely studied and is considered one of the most promising methods to obtain fermentable sugars. However, few data exist on the fermentation of reducing sugars (RS) obtained by enzymatic hydrolysis of biomass pretreated with ionic liquids for the production of ethanol. Therefore, this study evaluated the production of ethanol from sugars liberated from sawdust of Pinus sp. and Eucalyptus sp. pretreated with ionic liquid [C 4 mim][OAc] and [C 2 mim] [OAc], hydrolyzed with enzymes of Penicillium echinulatum employing Saccharomyces cerevisiae and Schizosaccharomyces pombe Y698 yeasts. The data indicate that when the biomass is pre-treated by [C 2 mim] [OAc] there is higher production of ethanol that, when treated by [C 4 mim][OAc] for both evaluated yeasts, even though the pretreatment with [C 2 mim] [OAc] caused the highest losses of cellulose and hemicellulose. Under the conditions analyzed, it is possible to produce approximately 40 and 47 L of ethanol per ton of Pinus sp. and Eucalyptus sp, respectively. In addition to contributing to knowledge about the physiology of the yeasts in the sugars liberated from biomass pretreated in presence of ionic liquid, this data is also relevant to the development of processes for the production of lignocellulosic ethanol.

Insight into the Factors for Separation of Lignin and Cellulose by Ionic Liquids Based on Molecular Simulation

“…It was found that the lignin content after pretreatment in [Choline]­[Gly] was much lower than [Choline]­[OAc]. Through our simulation, it is found that [Choline]­[Gly] has a better effect than [Choline]­[OAc] for the separation of cellulose and lignin, and the results are coincident with the reported experimental results. − Detailed data for composition changes of lignin and cellulose in the literatures can be found in Table S4. Molecular dynamics simulations were performed to study the separation mechanism of lignin and cellulose at the microscopic level, and the effects of different ILs on the separation of lignin and cellulose were analyzed.…”

Upcycling of tetra pack waste cellulose into reducing sugars for bioethanol production using Saccharomyces cerevisiae

“…Following GC analysis of the sample, the purity of bioethanol was found to be 96.47%. In a separate study, Tura et al (2018) reported the highest ethanol production from pre-treated lignocellulosic biomass by Schizosaccharomyces pombe at 35°C. Similarly, Dwivedi et al (2022) recorded a maximum ethanol production of 0.32 g/g after 48 hr of incubation using Schizosaccharomyces sp.…”

“…Millions of tons of tetra packs are discarded annually, constituting a signi cant portion of solid municipal waste, leading to environmental problems (Koh-Dzul et al, 2023). However, the potential utilization of this waste for valuable products, particularly bioethanol, is under investigation due to its wide availability, renewability, and costeffectiveness (Tura et al, 2018;Rathour et al, 2023). Production of bioethanol from tetra pack cellulose involves several steps, including pretreatment, enzymatic hydrolysis, microbial fermentation, and distillation, making it a viable biofuel alternative to petrol (Bhatia et al, 2017;Bhatt et al, 2018).…”

Energetic and Economic Analysis of Use of Hydrated Ethanol

“…The practical use of hydrated ethanol represents an essential factor for the reducing costs related to the energy consumed in the process (Venturini et al, 2018;Tura et al, 2018;Matugi et al, 2018;Robertson and Pavlath, 1985;Fagundez et al, 2017;Cataluña et al, 2017). The production of first and second-generation ethanol in the same industrial plant presents better financial results compared to the isolated processes (Silva et al, 2017;Caetano et al, 2015b;Dias et al, 2012;Ojeda et al, 2011;Seabra et al, 2010).…”

“…The production of first and second-generation ethanol in the same industrial plant presents better financial results compared to the isolated processes (Silva et al, 2017;Caetano et al, 2015b;Dias et al, 2012;Ojeda et al, 2011;Seabra et al, 2010). Also, ethanol obtained from the sugarcane has renewability, biodegradability and provides CO 2 emissions mitigation (Klunk et al, 2018a;Ponomarev et al, 2017;Caetano and Silva, 2017;Tura et al, 2018;Idrees et al, 2014;Banerji et al, 2014;Quintero et al, 2008).…”