Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

Fosso‐Kankeu, Elvis; Marx, Sanette; Brink, A.

doi:10.1590/0104-6632.20170342s20150491

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…As Gram-positive, endospore-forming bacteria, Bacillus spp. exhibit a wide range of physiological abilities which includes adaptation to biodiesel-diesel contamination 46 and active biodiesel degradation 47 . Differently from biodiesel- and diesel-contaminated soils, Rubrobacter , a known Actinobacteria well adapted for semi-arid soils 48 , was highest in control samples.…”

Section: Discussionmentioning

confidence: 99%

Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Mitter

Germida

Freitas

2021

Sci Rep

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Soil contamination as a result of oil spills is a serious issue due to the global demand for diesel fuel. As an alternative to diesel, biodiesel has been introduced based on its high degradability rates and potential for reducing of greenhouse gases emissions. This study assessed the impacts diesel and biodiesel contamination on soil microbial community activity and structure. Our results suggest higher microbial activity in biodiesel contaminated soils and analysis of PLFA profiles confirmed shifts in microbial community structure in response to contamination. High-throughput 16S rRNA amplicon sequencing also revealed a lower bacterial richness and diversity in contaminated soils when compared to control samples, supporting evidence of the detrimental effects of hydrocarbons on soil microbiota. Control samples comprised mostly of Actinobacteria, whereas Proteobacteria were predominantly observed in diesel and biodiesel contaminated soils. At genus level, diesel and biodiesel amendments highly selected for Rhodococcus and Pseudomonas spp., respectively. Moreover, predicted functional profiles based on hydrocarbon-degrading enzymes revealed significant differences between contaminated soils mostly due to the chemical composition of diesel and biodiesel fuel. Here, we also identified that Burkholderiaceae, Novosphingobium, Anaeromyxobacter, Pseudomonas and Rhodococcus were the main bacterial taxa contributing to these enzymes. Together, this study supports the evidence of diesel/biodiesel adverse effects in soil microbial community structure and highlights microbial taxa that could be further investigated for their biodegradation potential.

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Section: Discussionmentioning

confidence: 99%

Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Mitter

Germida

Freitas

2021

Sci Rep

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“…The control contained MSM and sterilized diesel oil 1% but without bacteria. The MSM was incubated under shaking condition at 150 rpm for 20 days at 37 o C (Fosso-Kankeu, Marx, & Brink, 2017). The extraction was done on day 0 and every 5 consecutive days.The diesel oil was extracted using liquid-liquid extraction of n-hexane with the equivalent volume of the mixture 1:1 (Latha & Kalaivani, 2012).…”

Section: Secondary Screening For Hydrocarbon Degradation Analysismentioning

confidence: 99%

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Arifin

Razali

Vigneswari

2021

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.

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“…As was reported by Soriano et al, in biodiesel and biodiesel blend 18 microbial genera (8 fungal and 10 bacterial) were identified by DNA sequencing, which have been associated with, among others, fuel microbial contamination and/or biodegradation, biofuel production or related enzymes' expression, biofilm formation and/or exopolysaccharide production [6]. Fosso-Kankeu et al studied two types of bacteria, which were exposed to different concentrations of diesel to increase their adaptation to the fossil fuel and were used for the degradation of dieselbiodiesel blends [7]. Owsianiak et al investigated the effect of surfactants on the microbial life of diesel/biodiesel blends [8].…”

Section: Introductionmentioning

confidence: 99%

Examination of Selected Physicochemical Properties of Biodiesel after Electron Beam Sterilization in Flow System

Grabowski

Jarosiński

2021

Energies

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The problem of microbial growth in fuels, especially biofuels, is a very important issue. Water is a necessity for microbial growth to occur. Unfortunately, it is very hard to exclude all water from fuel systems during daily operations, especially when biodiesel is added to the fuel mix. The aim of this work was to investigate the impact of ionizing radiation on selected properties of fatty acids methyl esters in order to evaluate the possibility of using irradiation techniques to sterilize biodiesel and its blends. Ionizing radiation influences the chemical composition of biodiesel samples containing especially unsaturated bonds. Irradiation was performed in a specially designed flow system. The tested parameters were compared with the normative values from the ISO 14214 standard. Density, kinematic viscosity, flash point, water content and cold filter plugging point as a macroscopic parameters do not change despite their irradiation with ionizing radiation. Change was observed in microscopic parameters as oxidation stability and esters content. During irradiation, Rapeseed Methyl Ester (RME) samples formed oxidation products, which lower the oxidative stability. It was observed that, for esters content, requirements of the standard could be met in the case of using very small doses. In the case of RME samples saturated with water, the changes in the ester concentration were smaller, but the resulting products decreased the oxidation stability. Sterilization fatty acids methyl esters (FAME) was observed with the use of e beam radiation is to apply very low doses (up to 2 kGy) in a flow system without adding water. Sterilization FAME saturated by water requires the use of higher doses of radiation, which adversely affects the oxidative stability.

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Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

Cited by 7 publications

References 32 publications

Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Examination of Selected Physicochemical Properties of Biodiesel after Electron Beam Sterilization in Flow System

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