LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Costa, Tania Maria; Hermann, Kátia Luiza; García-Román, Miguel; Valle, Rita de Cássia Siqueira Curto; Tavares, Lorena Benathar Ballod

doi:10.1590/0104-6632.20170342s20150477

Cited by 37 publications

(18 citation statements)

References 28 publications

(45 reference statements)

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“…SSF assays were carried out in 500‐mL flasks (cylindrical glass jar, 135 mm × 86.5 mm) containing 30 g of red rice and 90 mL of water. The moisture content of the SSF medium was adjusted to 51.0 ± 6.01% according to the method described in our previous work . The flasks with the medium were autoclaved (121 ºC for 30 Min) and inoculated with 20 mL of G. lipsiense mycelial fragments suspension, prepared according to the method described in our previous work .…”

Section: Methodsmentioning

confidence: 99%

“…The SSF mimics the natural habitat of filamentous fungi , ideal for the daily formation of some compounds, as well as for fruiting bodies in the cultivation of mushrooms. As part of ongoing studies to discover new bioactive compounds from basidiomycetes fungi , our team has identified some phenolic compounds produced by G. lipsiense using solid red rice medium . This cereal is rich in nutrients and some researchers have used this as a substrate for antioxidants, phenolic compounds, enzymatic inhibitors, enzymes, and coumarins production in process using fungi .…”

Section: Introductionmentioning

confidence: 99%

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Liposoluble compounds from Ganoderma lipsiense grown on solid red rice medium with antiparasitic and antibacterial properties

Costa

Lenzi

Paganelli

et al. 2019

Biotech and App Biochem

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Liposoluble molecules are a group of compounds that display potent biological and therapeutic properties. The present study aimed to identify liposoluble molecules produced by Ganoderma lipsiense grown in red rice medium using solid‐state fermentation (SSF) techniques, and to investigate the antigiardial and antibacterial activities potential of extracts in vitro. Eighteen fatty acids and derivatives were identified by gas chromatograph‐mass spectrometry (GC‐MS) analysis in G. lipsense extract. Qualitative (Fourier transform infrared spectroscopy and nuclear magnetic resonance) characterizations identified the steroid ergosta‐6,22‐diene‐3β,5α,8α‐triol in purified hexane subfraction (HEXsf) F19 isolated from hexane fraction (HEXf) of crude extract (CE). Ergosta‐6,22‐diene‐3β,5α,8α‐triol exhibited significant inhibitory activity against Giardia duodenalis throphozoites (93.6%) in in vitro assays. CE and HEXf inhibited 95.38% and 92.74% of the G. duodenalis throphozoites in 100 µg mL−1, whereas CE and their fractions dichloromethane (DCMf) and ethyl acetate (EAf) showed antibacterial activities against Pseudomonas aeruginosa and Staphylococcus aureus at 500 µg mL−1. Importantly, some liposoluble compounds produced and identified in G. lipsiense are unpublished for this species. This is first report for the production of ergosta‐6,22‐diene‐3β,5α,8α‐triol by G. lipsiense and its antiparasitic activity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Liposoluble compounds from Ganoderma lipsiense grown on solid red rice medium with antiparasitic and antibacterial properties

Costa

Lenzi

Paganelli

et al. 2019

Biotech and App Biochem

Self Cite

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“…The contemporary period machinery favors the procedure of batch fermentation and low cost extraction methods so the fungal lipases have assistances over bacteria. Major filamentous genera of fungi included are Rhizopus, Aspergillus, Penicillium, Mucor , Ashbya, Geotrichum, Beauveria , Humicola, Rhizomucor, Fusarium, Acremonium, Alternaria, Eurotrium and Ophiostoma for the production of lipases [ 123 , 124 ]. Other species such as Candida rugosa , Candida antarctica , T. lanuginosus , Rhizomucor miehei, Pseudomonas, Mucor and Geotrichum .…”

Section: Sources For Microbial Lipasesmentioning

confidence: 99%

Microbial lipases and their industrial applications: a comprehensive review

et al. 2020

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Lipases are very versatile enzymes, and produced the attention of the several industrial processes. Lipase can be achieved from several sources, animal, vegetable, and microbiological. The uses of microbial lipase market is estimated to be USD 425.0 Million in 2018 and it is projected to reach USD 590.2 Million by 2023, growing at a CAGR of 6.8% from 2018. Microbial lipases (EC 3.1.1.3) catalyze the hydrolysis of long chain triglycerides. The microbial origins of lipase enzymes are logically dynamic and proficient also have an extensive range of industrial uses with the manufacturing of altered molecules. The unique lipase (triacylglycerol acyl hydrolase) enzymes catalyzed the hydrolysis, esterification and alcoholysis reactions. Immobilization has made the use of microbial lipases accomplish its best performance and hence suitable for several reactions and need to enhance aroma to the immobilization processes. Immobilized enzymes depend on the immobilization technique and the carrier type. The choice of the carrier concerns usually the biocompatibility, chemical and thermal stability, and insolubility under reaction conditions, capability of easy rejuvenation and reusability, as well as cost proficiency. Bacillus spp., Achromobacter spp., Alcaligenes spp., Arthrobacter spp., Pseudomonos spp., of bacteria and Penicillium spp., Fusarium spp., Aspergillus spp., of fungi are screened large scale for lipase production. Lipases as multipurpose biological catalyst has given a favorable vision in meeting the needs for several industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. This review represents a discussion on microbial sources of lipases, immobilization methods increased productivity at market profitability and reduce logistical liability on the environment and user.

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“…Agro-industrial wastes can easily be degraded by filamentous fungi, when synthesizing industrially important bio-compounds, such as lipolytic enzymes (34) . It is known that various fats, fatty acids, plant oils, triglycerides, ester-based detergents, and other substances are the best inducers of lipase synthesis by microorganisms and sources of carbon (35) .…”

Section: Quantitative Analysis Of Lipase Enzyme Under Submerges Fermementioning

confidence: 99%

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

Patel¹,

Shah²

2020

IJST

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Background/Objectives: The present study was focused to exploit the indigenous strains of fungi isolated from cotton seed soapstock for the production of the extracellular lipase through submerged fermentation technique. Methods/ Statistical analysis: Cotton seed soapstock samples used in the study contains gelatinous oil richer chemical constituents. In addition, their enrichment and diluted materials were used for the isolation of lipase producing microorganisms on tributyrin agar plates. All isolates were lipase positive confirmed by a qualitative plate assay. Quantitative estimation of Lipase production activity was measured spectrophotometrically using pnitrophenyl palmitate (p-NPP) as a substrate. In order to exploit the isolated fungal strain for industrial usage, other cellulase and protease enzymes were tested by plate assay. Morphological and molecular characteristics of selected isolates were studied. Findings: From enriched soapstock samples, a total of 49 cultures were isolated among them 19 fungal isolates were screened for lipase, cellulase, and protease activity qualitatively by plate assay. Out of 19, six fungi were selected based on their lipase activity. Highly potent Fusarium solani 7F had the ability to produce 5.95 U/mL/min. crude lipase whereas Aspergillus niger 13F has 4.2 U/mL/min. after 4days incubation at 30oC. Potent fungi culture was identified by morphological, cultural, and molecular characteristics (18s rRNA gene sequence and phylogenetic analysis) revealed them as Penicillium griseofulvum 5F, Aspergillus flavus 6F, Fusarium solani 7F, Aspergillus niger 12F, Aspergillus niger 13F, and Aspergillus terreus 17F. Novelty: Fungi was the first time reported and isolated from cotton seed soapstock materials. In future studies, this enzyme will be used in the degradation of soapstock and also in the production of biosurfactant from soapstock. Keywords: Cotton seed soapstock; Fusarium solani 7F; p-nitrophenyl palmitate; Tributyrin agar plates; degradation

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LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Cited by 37 publications

References 28 publications

Liposoluble compounds from Ganoderma lipsiense grown on solid red rice medium with antiparasitic and antibacterial properties

Liposoluble compounds from Ganoderma lipsiense grown on solid red rice medium with antiparasitic and antibacterial properties

Microbial lipases and their industrial applications: a comprehensive review

Isolation, screening and identification of Lipase producing fungi from cotton seed soapstock

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