BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Pirota, Rosangela Donizete Perpetua Buzon; Tonelotto, Mariana; Delabona, P. S.; Fonseca, R. F.; Paixão, Douglas Antônio Alvaredo; Baleeiro, Flávio C. F.; Neto, V. Bertucci; Farinas, Cristiane S.

doi:10.1590/0104-6632.20160331s00003520

Cited by 18 publications

(4 citation statements)

References 31 publications

(32 reference statements)

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“…Currently, it is industrially interesting to produce cellulase cocktails. Therefore, many of them have been studied with applications in several industries, such as paper and pulp, textile, detergent, food, juice, chemical reagents, agriculture, and the production of biofuels from lignocellulosic biomass [71,97,98] . Several authors have presented the potential effect of enzymatic cocktails to further improve the production and properties of NC, [61,89] and recently, it has also been demonstrated that enzymatic hydrolysis with customized blends has also been demonstrated that enzymatic hydrolysis produces nanocellulose with applications in cosmetic and pharmaceutical fields [99,100] …”

Section: Cellulases As Potential Tools For Nanocellulose Productionmentioning

confidence: 99%

Nanocellulose: Production and Processing for Biomedical Applications

et al. 2021

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Nanocellulose (NC) is a unique and promising natural material derived from native cellulose. It has attracted much attention for its versatility in construction composites, which have improved chemical and mechanical properties. This review covers the type of biomass used and NC production methods, emphasizing biotechnological routes, which promote their biological properties (biocompatibility, biodegradability and low toxicity), fundamental for medical applications. NC has many hydroxyl groups on its surface, enabling its structure to be modifiable, adaptable and viable for various fields. Furthermore, NC‐based biosensors are suitable for food quality control, monitoring environmental analytes, and particularly in health diagnostics, such as COVID19 detection. The developed analytical devices were described in a table, including information about the sensor molecule, reading system, analyte, range, detection limit, sensitivity and analytical performance.

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Section: Cellulases As Potential Tools For Nanocellulose Productionmentioning

confidence: 99%

Nanocellulose: Production and Processing for Biomedical Applications

et al. 2021

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“…Aspergillus oryzae MUCL 14492 was employed in screening on solid medium as a reference culture. This culture has been extensively used before particularly for synthesis of cellulase, tannase and pectinolytic enzymes (Heerd et al 2012;Pirota et al 2016;Koseki et al 2018). There were no significant differences between levels of cellulase and pectinase displayed on solid medium by the isolates and the reference culture.…”

Section: Screening Of Isolates For Enzyme Production On Solid Mediummentioning

confidence: 99%

Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape

Gülsünoğlu¹,

Kılıç‐Akyılmaz²,

Karbancıoğlu-Güler³

2019

Tarım Bilimleri Dergisi

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Black aspergilli newly isolated from local grape and date were investigated for production of hydrolytic enzymes including cellulase, tannase and pectinase. Isolates were morphologically and molecularly identified as Aspergillus niger, Aspergillus tubingensis, Aspergillus japonicus and Aspergillus aculeatus. Isolates were screened for enzyme production ability on solid and in liquid media. Enzymatic activity was determined in the culture filtrate of liquid medium. A total of six isolates were found to produce multiple hydrolytic enzymes. The highest activity of cellulase was produced by A. japonicus ZGM4 and A. aculeatus ZGM6 as 40 and 35 U g -1 dry biomass, respectively. All the isolates exhibited high level of tannase activity in the range of 150-343 U g -1 dry biomass after 24 h of incubation. A. tubingensis ZGM5 and A. aculeatus ZGM6 were found to produce the highest pectinase activity at a level of 130 and 117 U g -1 dry biomass, respectively. In the light of these results, isolates can be used for multiple hydrolytic enzyme production in industry.

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“…The A. oryzae and A. niger are important potent endoglucanase producers, usually employed in various industrial and biotechnological applications. They also produce other enzymes for the degradation of plant polysaccharides such as pectin, xyloglucan, xylan and galactomannan [19,20]. The Koji (A. oryzae)…”

Section: Introductionmentioning

confidence: 99%

Gamma Rays Mediated Mutagenesis of Super Koji (Aspergillus oryzae) to enhance the Thermostability and Production of CMCases: Process Scale-up at Pilot Level

Zeb¹,

Rashid²,

Aleem³

et al. 2019

Preprint

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Background The β-1, 4-endoglucanase belongs to cellulases group of enzymes, which hydrolyse β-1, 4 glucosidic bonds in the naturally occurring cellulose or synthetic Carboxymethyl cellulose CMC thus also named as CMCase. Endoglucanase from Aspergillus oryzae is favoured in food and feed industries due to its GRAS (generally regarded as safe) status and for relatively stable enzymes production. Results Current report is novel as it deals with the development of super Koji strain having hyper production of thermostable CMCases. Hence, the potent Koji’s mutant, hyper producer of thermostable endoglucanase was screened from the available mutants stock (Total = 52 variants), which was generated through gamma rays treatment of Super Koji strain (A. oryzae cmc1). Initially, seventeen (17) mutants hyper producer of CMCases were selected based on the basis of clearance zone index (CI) on the plates supplemented with 1% CMC. Afterwards, six mutants resistant to 2-deoxy-D-glucose were selected having enhanced CMCase production. Mutants’ next screening was on submerged CMCase production. Potent mutant 60(5) having 60 kRad γ-rays exposure showed 3.6 folds hyper CMCase production (6.02 U ml-1). The half-life of endoglucanse produce of A. oryzae mutant 60(5) was 29.42 min and 14.8 min at 55 ⁰C and 60 ⁰C, respectively, which was 4 folds higher than the control at 60 ⁰C. The LC-MS analysis showed that A. oryzae mutant 60(5) does not produce aflatoxins. Field Emission Scanning Electron Microscopy (FESM) of mutated Koji depicted that gamma radiations enhanced the stiffness of fungal mycelia. Kinetics of CMCase production by mutant 60(5) in 10 L and 200L fermenter was as: CMCase = 913 U dl-1 & 821 U dl-1; Specific growth (µ) = 0.064 & 0.082 hours; Cell mass doubling time (td) = 10.71 & 8.42 hours; Yield coefficient with respect to cell mass (Yp/x) = 59 Ug-1 & 89 U g-1 and Specific rate of CMCase production (qp) = 3.84 Ug-1h-1 & 7.37 Ug-1h-1 respectively. Conclusion We concluded, γ-rays induced potent mutations in mutant 60(5) resulting in hyper production of thermostable endoglucanase, which can withstand harsh industrial conditions. Hence, has great potential for applications in food & feed and other industrial processes.

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BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Cited by 18 publications

References 31 publications

Nanocellulose: Production and Processing for Biomedical Applications

Nanocellulose: Production and Processing for Biomedical Applications

Production of Multiple Hydrolytic Enzymes by Black Aspergilli Isolated from Date and Grape

Gamma Rays Mediated Mutagenesis of Super Koji (Aspergillus oryzae) to enhance the Thermostability and Production of CMCases: Process Scale-up at Pilot Level

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