EFFECTS OF CULTIVATION MEDIA COMPONENTS ON BIOSURFACTANT AND PIGMENT PRODUCTION FROM Pseudomonas aeruginosa PAO1

Das, Subhasish; Das, Palashpriya

doi:10.1590/0104-6632.20150322s00003262

Cited by 11 publications

(12 citation statements)

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“…might be due to siderophores production which can enhance its acquisition of iron (Cheng et al . ) and pigment pyocyanin, a kind of toxin which can kill other competitor micro‐organisms (Das and Das ).…”

Section: Discussionmentioning

confidence: 99%

“…Inhibitory ability of Pseudomonas sp. might be due to siderophores production which can enhance its acquisition of iron (Cheng et al 1995) and pigment pyocyanin, a kind of toxin which can kill other competitor micro-organisms (Das and Das 2015). Unlike planktonic populations, the antagonistic interactions between Salmonella and P. aeruginosa in the dual-species biofilms resulted in lower biofilm cell densities of both species compared to individual mono-species biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Biofilm formation and disinfectant resistance ofSalmonellasp. in mono- and dual-species withPseudomonas aeruginosa

2017

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Biofilm formation and disinfectant resistance ofSalmonellasp. in mono- and dual-species withPseudomonas aeruginosa

2017

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“…All evaluated P. putida strains were able to some extent to reduce the attachment and biofilm formation by S. Java, supporting the potential of P. putida as a BCA against S. Java in the DWS of broiler houses. The ability of Pseudomonas strains to inhibit the growth of several pathogenic bacteria, among which Salmonella, was previously attributed to the production of iron-capturing siderophores and the toxic pigment pyocyanin [ 11 , 17 , 24 , 29 , 45 ]. P. putida specifically also produces several biosurfactants that can inhibit biofilm formation and even break down existing biofilms [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas putida as a potential biocontrol agent against Salmonella Java biofilm formation in the drinking water system of broiler houses

et al. 2020

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Background Environmental biofilms can induce attachment and protection of other microorganisms including pathogens, but can also prevent them from invasion and colonization. This opens the possibility for so-called biocontrol strategies, wherein microorganisms are applied to control the presence of other microbes. The potential for both positive and negative interactions between microbes, however, raises the need for in depth characterization of the sociobiology of candidate biocontrol agents (BCAs). The inside of the drinking water system (DWS) of broiler houses is an interesting niche to apply BCAs, because contamination of these systems with pathogens plays an important role in the infection of broiler chickens and consequently humans. In this study, Pseudomonas putida, which is part of the natural microbiota in the DWS of broiler houses, was evaluated as BCA against the broiler pathogen Salmonella Java. Results To study the interaction between these species, an in vitro model was developed simulating biofilm formation in the drinking water system of broilers. Dual-species biofilms of P. putida strains P1, P2, and P3 with S. Java were characterized by competitive interactions, independent of P. putida strain, S. Java inoculum density and application order. When equal inocula of S. Java and P. putida strains P1 or P3 were simultaneously applied, the interaction was characterized by mutual inhibition, whereas P. putida strain P2 showed an exploitation of S. Java. Lowering the inoculum density of S. Java changed the interaction with P. putida strain P3 also into an exploitation of S. Java. A further increase in S. Java inhibition was established by P. putida strain P3 forming a mature biofilm before applying S. Java. Conclusions This study provides the first results showing the potential of P. putida as BCA against S. Java in the broiler environment. Future work should include more complex microbial communities residing in the DWS, additional Salmonella strains as well as chemicals typically used to clean and disinfect the system.

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“…All evaluated P. putida strains were able to some extent to reduce the attachment and bio lm formation by S. Java, supporting the potential of P. putida as a BCA against S. Java in the DWS of broiler houses. The ability of Pseudomonas strains to inhibit the growth of several pathogenic bacteria, among which Salmonella, was previously attributed to the production of iron-capturing siderophores and the toxic pigment pyocyanin (Oblinger and Kraft, 1970;Gram, 1993;Cheng et al, 1995;Das and Das, 2015;Khare and Tavazoie, 2015). P. putida speci cally also produces several biosurfactants that can inhibit bio lm formation and even break down existing bio lms (Kuiper et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas putida as a potential biocontrol agent against Salmonella Java biofilm formation in the drinking water system of broiler houses

Maes

Reu

Weyenberg

et al. 2020

Preprint

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Background: Environmental biofilms can induce attachment and protection of other microorganisms including pathogens, but can also prevent them from invasion and colonization. This opens the possibility for so-called biocontrol strategies, wherein microorganisms are applied to control the presence of other microbes. The potential for both positive and negative interactions between microbes, however, raises the need for in depth characterization of the sociobiology of candidate biocontrol agents (BCAs). The inside of the drinking water system (DWS) of broiler houses is an interesting niche to apply BCAs, because contamination of these systems with pathogens plays an important role in the infection of broiler chickens and consequently humans. In this study, Pseudomonas putida, which is part of the natural microbiota in the DWS of broiler houses, was evaluated as BCA against the broiler pathogen Salmonella Java.Results: To study the interaction between these species, an in vitro model was developed simulating biofilm formation in the drinking water system of broilers. Dual-species biofilms of P. putida strains P1, P2, and P3 with S. Java were characterized by competitive interactions, independent of P. putida strain, S. Java inoculum density and application order. When equal inocula of S. Java and P. putida strains P1 or P3 were simultaneously applied, the interaction was characterized by mutual inhibition, whereas P. putida strain P2 showed an exploitation of S. Java. Lowering the inoculum density of S. Java changed the interaction with P. putida strain P3 also into an exploitation of S. Java. A further increase in S. Java inhibition was established by P. putida strain P3 forming a mature biofilm before applying S. Java.Conclusions: This study provides the first results showing the potential of P. putida as BCA against S. Java in the broiler environment. Future work should include more complex microbial communities residing in the DWS, additional Salmonella strains as well as chemicals typically used to clean and disinfect the system.

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EFFECTS OF CULTIVATION MEDIA COMPONENTS ON BIOSURFACTANT AND PIGMENT PRODUCTION FROM Pseudomonas aeruginosa PAO1

Cited by 11 publications

References 12 publications

Biofilm formation and disinfectant resistance ofSalmonellasp. in mono- and dual-species withPseudomonas aeruginosa

Biofilm formation and disinfectant resistance ofSalmonellasp. in mono- and dual-species withPseudomonas aeruginosa

Pseudomonas putida as a potential biocontrol agent against Salmonella Java biofilm formation in the drinking water system of broiler houses

Pseudomonas putida as a potential biocontrol agent against Salmonella Java biofilm formation in the drinking water system of broiler houses

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