Error propagation in open respirometric assays

Lobo, Cintia Cecilia; Bértola, Nora Cristina; Contreras, Edgardo Martín

doi:10.1590/0104-6632.20140312s00002659

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Based on the values of q Sm (Table 1), the tested phenolic compounds can be ordered as follows: CA > PH >> RE > HY. This order of metabolism rates agrees with other authors that studied the oxygen consumption rate in the presence of phenolic compounds by intact cells, and enzyme (cell-free) preparations (Ahuatzi-Chacón et al, 2004;Lepik and Tenno, 2011;Lobo et al, 2013Lobo et al, , 2014Orupõld et al, 2001). Table 1 shows that the values corresponding to the coefficients K S , and K i had relative errors ranging from 30 to 53%.…”

Section: Modelling the Oxidation Of Single Substrates And Binary Mixtsupporting

confidence: 89%

“…The operation temperature was 20 ± 1 °C. Before the addition of the tested compound, the oxygen mass transfer coefficient of the respirometer (k L a) and the endogenous oxygen uptake rate (OUR end ) were obtained using a non-steady state procedure (Lobo et al, 2014). According to the experimental conditions, such as biomass concentration and the compound tested, the k L a values employed ranged between 10 and 40 h -1…”

Section: Respirometric Assaysmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition Kinetics During the Oxidation of Binary Mixtures of Phenol With Catechol, Resorcinol and Hydroquinone by Phenol Acclimated Activated Sludge

Lobo

Bértola

Contreras

2016

Braz. J. Chem. Eng.

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-In this work the aerobic degradation of phenol (PH), catechol (CA), resorcinol (RE), hydroquinone (HY) and of the binary mixtures PH+CA, PH+RE, PH+HY by phenol-acclimated activated sludge was studied. Single substrate experiments show a Haldane-type dependence of the respiration rate on PH, RE and HY, while CA corresponded to the Monod model. Binary substrate experiments demonstrated that the presence of a second substrate only affected the kinetics, but not the stoichiometry of the oxidation of the compounds tested. While CA inhibited the oxidation of PH, PH inhibited the oxidation of RE and HY. A mathematical model was developed to represent the aerobic biodegradation of the phenolic compounds tested. The agreement between the proposed model and the experimental data indicates that the proposed model can be useful for predicting substrate and dissolved oxygen concentrations in bioreactors treating phenolic wastewaters.

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Section: Modelling the Oxidation Of Single Substrates And Binary Mixtsupporting

confidence: 89%

Section: Respirometric Assaysmentioning

confidence: 99%

Inhibition Kinetics During the Oxidation of Binary Mixtures of Phenol With Catechol, Resorcinol and Hydroquinone by Phenol Acclimated Activated Sludge

Lobo

Bértola

Contreras

2016

Braz. J. Chem. Eng.

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“…Before the addition of the tested compounds (BAC100 or BAC100/HF), the oxygen mass transfer coefficient of the respirometer (kLa) was obtained using a non-steady state procedure (Lobo et al, 2014).…”

Section: Respirometric Assaysmentioning

confidence: 99%

Fenton Oxidation as a Strategy to Preserve the Biomass in the Activated Sludge System

Doumic¹,

Almeida²,

Giulietti³

et al. 2020

LAAR

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Wastewaters containing biocides constitute an increasing environmental pollution concern. In this work, the feasibility of implementing a pre-treatment based on the Fenton process to minimize the negative impact of biocide compounds, such as benzalkonium chlorides (BAC), on the activated sludge system was studied. The behavior of the microbial community in the presence of a BAC solution untreated (BAC100) or pre-treated by homogeneous Fenton oxidation (BAC100/HF) was analyzed. The addition of BAC100 to the activated sludge system caused a deleterious effect. The cellular lysis process caused a 30% decrease in biomass concentration. The inhibition of respiratory activity was 73%. In contrast, BAC100/HF caused no significant decrease in the biomass concentration or detectable inhibition of the respiratory activity. Besides, the consumption of easily oxidizable products was observed. Results indicated that the homogeneous Fenton process as a pre-treatment of wastewaters containing BAC could become a feasible alternative to protect active biomass in biological treatment systems.

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“…A respirometric technique (Lobo et al 2013(Lobo et al , 2014 was used to evaluate the metabolic activity of the activated sludge in all the tested reactors (G1, A1, P1, G2, A2 or P2). Reactors were continuously aerated at a stable flow rate (1.0 L min -1 ) using a high precision rotameter (Bruno Schilling model MB 60 V, Argentina).…”

Section: Respirometric Measurementsmentioning

confidence: 99%

“…The DO concentration (C) as a function of time (t) was recorded every 5 s using an optical DO probe (YSI ProODO). Before the addition of the tested substrate (glucose, acetate, peptone), the oxygen mass transfer coefficient of the respirometer (k L a) was obtained using a non-steady state procedure (Lobo et al 2014). When a stable dissolved oxygen concentration (C) was observed, the reactor was spiked with the corresponding biogenic substrate (glucose, acetate or peptone, 500 mgCOD L -1 ) and C as a function of time (t) was recorded.…”

Section: Respirometric Measurementsmentioning

confidence: 99%

Interdependence between the aerobic degradation of BPA and readily biodegradable substrates by activated sludge in semi-continuous reactors

2018

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The objective of the present work was to analyze the interrelationship between the aerobic degradation of BPA and readily biodegradable substrates by activated sludge (AS) in semi-continuous reactors (SCRs). AS were obtained from three SCRs fed with glucose, acetate or peptone. AS from these reactors were used as inocula for three SCRs that were fed with each biogenic substrate, and for three SCRs that were fed with the biogenic substrate and BPA. In all cases, dissolved organic carbon (DOC), BPA, total suspended solids (TSS) and respirometric measurements were performed. Although BPA could be removed in the presence of all the tested substrates, AS grown on acetate exhibited the longest acclimation to BPA. Reactors fed with peptone attained the lowest TSS concentration; however, these AS had the highest specific BPA degradation rate. Specific DOC removal rates and respirometric measurements demonstrated that the presence of BPA had a negligible effect on the removal of the tested substrates. A mathematical model was developed to represent the evolution of TSS and DOC in the SCRs as a function of the operation cycle. Results suggest that the main effect of BPA on AS was to increase the generation of microbial soluble products. This work helps to understand the relationship between the biodegradation of BPA and readily biodegradable substrates.

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Error propagation in open respirometric assays

Cited by 8 publications

References 25 publications

Inhibition Kinetics During the Oxidation of Binary Mixtures of Phenol With Catechol, Resorcinol and Hydroquinone by Phenol Acclimated Activated Sludge

Inhibition Kinetics During the Oxidation of Binary Mixtures of Phenol With Catechol, Resorcinol and Hydroquinone by Phenol Acclimated Activated Sludge

Fenton Oxidation as a Strategy to Preserve the Biomass in the Activated Sludge System

Interdependence between the aerobic degradation of BPA and readily biodegradable substrates by activated sludge in semi-continuous reactors

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