Maintenance-energy-dependent dynamics of growth and poly(3-hydroxybutyrate) [P(3HB)] production by Azohydromonas lata MTCC 2311 using simple and renewable carbon substrates

Zafar, Mohd.; Kumar, Shashi; Dhiman, Amit; Park, Hung‐Suck

doi:10.1590/0104-6632.20140312s00002434

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…In particular, the C/N and C/P mass ratios were set at 10 w/w and 1.9 w/w, respectively, while the culture volumes were harvested when RT was equal to 1 h. The incubation frequency was constant at 210 rpm, so DO was not controlled in this series of experiments. As confirmed by the results in Table 1, A. lata is indeed able to grow on and utilize different sugar substrates, even if they are mono-or di-saccharides [51]. On the contrary, there are clear experiments, such as those with lactose (L), xylose (X), fructose (F), and cheese whey (CW), in which the bacteria could not fully metabolize the available carbon source and produce sufficient amounts of biomass and PHB.…”

Section: Evaluation Of Cheese Whey Conversion To Phbsupporting

confidence: 67%

Microbial Conversion of Cheese Whey to Polyhydroxybutyrate (PHB) via Statistically Optimized Cultures

2023

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The intended circular economy for plastics envisages that they will be partially replaced by bio-based polymers in the future. In this work, the natural polyester polyhydroxybutyrate (PHB) was produced by Azohydromonas lata using cheese whey (CW) as a low-cost substrate. Initially, CW was evaluated as the sole carbon source for PHB production; it was found to be efficient and comparable to PHB production with pure sugars, such as saccharose or glucose, even when mild (with dilute acid) hydrolysis of cheese whey was performed instead of enzymatic hydrolysis. An additional series of experiments was statistically designed using the Taguchi method, and a dual optimization approach was applied to maximize the intracellular biopolymer content (%PHB, selected as a quantitative key performance indicator, KPI) and the weight average molecular weight of PHB (Mw, set as a qualitative KPI). Two different sets of conditions for the values of the selected bioprocess parameters were identified: (1) a carbon-to-nitrogen ratio (C/N) of 10 w/w, a carbon-to-phosphorous ratio (C/P) of 1.9 w/w, a dissolved oxygen concentration (DO) of 20%, and a residence time in the stationary phase (RT) of 1 h, resulting in the maximum %PHB (61.66% w/w), and (2) a C/N of 13.3 w/w, a C/P of 5 w/w, a DO of 20%, and a RT of 1 h, leading to the maximum Mw (900 kDa). A final sensitivity analysis confirmed that DO was the most significant parameter for %PHB, whereas C/N was the most important parameter for Mw.

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Section: Evaluation Of Cheese Whey Conversion To Phbsupporting

confidence: 67%

Microbial Conversion of Cheese Whey to Polyhydroxybutyrate (PHB) via Statistically Optimized Cultures

2023

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“…Any attempt of reducing CO 2 emission in the atmosphere can be a milestone to protecting the natural ecosystem. In addition, strain G-1-1-14 T produces P3(HB) (and regulatory proteins WP_169159239, WP_169160336, and WP_169163299; Supplementary Figure 6), which is also responsible for reducing the CO 2 releases in the atmosphere (Zafar et al, 2014). Species of Azohydromonas have been revealed to synthesize various polyβ-hydroxyalkanoates (PHAs) (Zafar et al, 2014;Sharma et al, 2017).…”

Section: Genome Analysismentioning

confidence: 99%

“…In addition, strain G-1-1-14 T produces P3(HB) (and regulatory proteins WP_169159239, WP_169160336, and WP_169163299; Supplementary Figure 6), which is also responsible for reducing the CO 2 releases in the atmosphere (Zafar et al, 2014). Species of Azohydromonas have been revealed to synthesize various polyβ-hydroxyalkanoates (PHAs) (Zafar et al, 2014;Sharma et al, 2017). PHAs have been widely applied for the production of biodegradable and biocompatible plastics (Zafar et al, 2014).…”

Section: Genome Analysismentioning

confidence: 99%

“…Species of Azohydromonas have been revealed to synthesize various polyβ-hydroxyalkanoates (PHAs) (Zafar et al, 2014;Sharma et al, 2017). PHAs have been widely applied for the production of biodegradable and biocompatible plastics (Zafar et al, 2014). From this point of view, strain G-1-1-14 T can be considered as a promising bioresource to mitigate plastic-associated problems and environmental issues.…”

Section: Genome Analysismentioning

confidence: 99%

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Azohydromonas caseinilytica sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method

et al. 2021

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A bacterial strain, designated strain G-1-1-14T, was isolated from Kyonggi University forest soil during a study of previously uncultured bacterium. The cells of strain G-1-1-14T were motile by means of peritrichous flagella, Gram-stain-negative, rod-shaped, and able to grow autotrophically with hydrogen and fix nitrogen. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain G-1-1-14T belonged to the genus Azohydromonas. The closest species of strain G-1-1-14T were Azohydromonas ureilytica UCM-80T (98.4% sequence similarity), Azohydromonas lata IAM 12599T (97.5%), Azohydromonas riparia UCM-11T (97.1%), and Azohydromonas australica IAM 12664T (97.0%). The genome of strain G-1-1-14T was 6,654,139 bp long with 5,865 protein-coding genes. The genome consisted of N2-fixing genes (nifH) and various regulatory genes for CO2 fixation and H2 utilization. The principal respiratory quinone was ubiquinone-8, and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol. The major fatty acids were summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), C16:0, summed feature 8 (C18:1ω7c and/or C18:1ω6c), and cyclo-C17:0. The DNA G + C content was 69.9%. The average nucleotide identity (OrthoANI), in silico DNA–DNA hybridization (dDDH), and conventional DDH relatedness values were below the species demarcation values for novel species. Based on genomic, genetic, phylogenetic, phenotypic, and chemotaxonomic characterizations, strain G-1-1-14T represents a novel species within the genus Azohydromonas, for which the name Azohydromonas caseinilytica sp. nov. is proposed. The type strain is G-1-1-14T (= KACC 21615T = NBRC 114390T).

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Characterization of polyhydroxyalkanoates extracted from wastewater sludge under different environmental conditions

Cha

Son

Jamal

et al. 2016

Biochemical Engineering Journal

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Maintenance-energy-dependent dynamics of growth and poly(3-hydroxybutyrate) [P(3HB)] production by Azohydromonas lata MTCC 2311 using simple and renewable carbon substrates

Cited by 6 publications

References 9 publications

Microbial Conversion of Cheese Whey to Polyhydroxybutyrate (PHB) via Statistically Optimized Cultures

Microbial Conversion of Cheese Whey to Polyhydroxybutyrate (PHB) via Statistically Optimized Cultures

Azohydromonas caseinilytica sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method

Characterization of polyhydroxyalkanoates extracted from wastewater sludge under different environmental conditions

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