Additive effects of CuSO4 and aromatic compounds on laccase production by Pleurotus sajor-caju PS-2001 using sucrose as a carbon source

Abstract

-Laccase enzymes are now commercially available, and a laccase/mediator combination is currently marketed for indigo dye bleaching in textile manufacturing; replacing traditional chemical-based processes with enzymatic technology reduces the need for effluent treatment. However, an inexpensive source of these enzymes will be needed to enable wider application of this technology. In the present work, the main objective was to increase laccase production by the mushroom Pleurotus sajor-caju strain PS-2001 grown on sucrose derived from sugar cane, one of most economical carbon sources known, by the addition of compounds that are known to affect laccase production. High laccase activities (45-62 U mL -1 ) were obtained with additions of syringaldazine, benzoic acid, gallic acid, and vanillin. When CuSO 4 was used in conjunction with these aromatic compounds, the levels of laccase activity were further improved, reaching 58-80 U mL -1 . These laccase activities indicate the potential of this strain as an enzyme producer, which has also been detected in media containing glucose, but with activity lower than that observed with sucrose.

Influence of Oxygen Supply on Growth and Laccases Production by Pleurotus sajor-caju PS-2001 in Submerged Process

“…The culture medium for inocula preparation and bioreactor experiments contained (per liter): glucose, 5 g; pure casein, 1.5 g; CuSO4, 100 mg; benzoic acid, 100 mg; mineral solution, 100 mL [29]. The mineral solution was composed of (per liter): 20 g KH2PO4, 14 g (NH4)2SO4, 3 g MgSO4.7H2O, 3 g urea, 3 g CaCl2, 15.6 g MnSO4.H2O, 50 mg FeSO4, 14 mg ZnSO4, and 20 mg CoCl2.…”

“…During the experiments, three different conditions of oxygen concentration (DO) were tested (uncontrolled, 30% and 80% of saturation in the medium), using basic medium constituted of glucose, pure casein and mineral solution added of CuSO4 and benzoic acid as enzymatic inducers at pH controlled between 4.5 and 7.0 [28,29]. Figure 1 shows laccases activities, substrate and biomass concentrations during the 168 hours of the experiment.…”

“…Pleurotus sajor-caju PS-2001 shows optimal growth in liquid culture [24]. It is also an important laccases producer [27][28][29].…”

Effects of pH, Temperature and Agitation on the Decolourisation of Dyes by Laccase-Containing Enzyme Preparation from Pleurotus sajor-caju

“…For the production of laccase-containing enzyme broth, the following cultivation medium was used (per litre): glucose, 5.0 g; pure casein (Synth ® ), 1.5 g; CuSO4, 100 mg; benzoic acid, 100 mg; mineral solution, 100 mL [32]. The cultivations were carried out in a stirred-tank bioreactor B. Braun Biotech model Biostat ® B with 4.0 L of working volume.…”

The effect of various inducers and their combinations with copper on laccaseproduction of Trametes versicolor pellets in a repeated-batch process

“…Due to their broad industrial and environmental uses, these enzymes have gained increasing attention. Laccase production can be stimulated by various inductive substances, including metal ions, aromatic or phenolic compounds, alcohol, and detergents (Collins and Dobson, 1997;Birhanli and Yesilada, 2006;Tychanowicz et al, 2006;Bettin et al, 2014). Furthermore, inducer concentration, fermentation types, growth media, and incubation conditions have an important effect on laccase production and they are specific to species and strains (Boran and Yesilada, 2011).…”