Extraction of poly(3-hydroxybutyrate) from Spirulina LEB 18 for developing nanofibers

Abstract

SbstractThe objective of this study was to extract poly(3-hydroxybutyrate) (PHB) from the microalgal biomass of Spirulina LEB 18 for the development of nanofibers by electrospinning method. Different extraction methods were tested. The maximum yield obtained was 30.1 ± 2%. It was possible to produce nanofibers with diameters between 826 ± 188 nm and 1,675 ± 194 nm. An increase in the nanofiber diameter occurred when a flow rate of 4.8 μL min -1 and a capillary diameter of 0.90 mm were used. The nanofibers produced had up to 34.4% of biomass additives, i.e., non-PHB materials. This can be advantageous, because it enables the conservation of microalgal biomass compounds with bioactive functions.

Physicochemical characteristics of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) electrospun nanofibres for the adsorption of phenol

“…This paper also reported the average fibre diameters were found to be decreased from 1.59 to 1.17 lm when the applied voltage is increased [7]. Another recent study by Kuntzler et al [16] and Morais et al [17], the optimum conditions of their experiment was processed at 15 kV electric potential, and 0.45 mm diameter of the extrusion capillary. Reduced nanofibre diameter was caused by the superior conductivity of the solution.…”

Microalgae Biomolecules: Extraction, Separation and Purification Methods

“…Thus, GPC gives the molecular weight distribution of a sample [125,147]. For microalgae, this technique is particularly useful in the characterization of polysaccharides [148,149] that can reach levels in the range of 8-64% DW [150] and polyhydroxyalkanoates [151].…”

Cyanobacterial PHA Production—Review of Recent Advances and a Summary of Three Years’ Working Experience Running a Pilot Plant

“…Nevertheless, it must be emphasized that Arthrospira , at an industrial scale, is still one of the most promising candidates for PHB production with cyanobacteria. Indeed, PHB nanofibers were produced recently from Arthrospira PHB and showed highly favourable properties [ 58 , 59 ]. The biggest challenge for further research is to increase the relatively low PHB content of Arthrospira .…”