The use of lysozyme to prepare biologically active chitooligomers

Abstract

SbstractTwo types of crustacean commercial chitosans (CS1, CS2) were dissolved in lactic acid solutions, hydrolysed by lysozyme and finally fractioned by methanol solutions into two parts containing chito-oligomers (CS-O1, CS-O2). The antioxidant power and antimicrobial properties of both fractions were studied and compared with non-hydrolysed CS1 and CS2. The antioxidant properties were determined by the ferric ion reducing antioxidant power (FRAP) method while the bioactive properties were evaluated against a strain of Listeria monocytogenes. CS-O obtained from the solid fraction of the chito-oligomers solid fractions treated with 90% methanol showed the highest reducing power. Microbiological tests showed that CS-O exhibit higher antilisterial activity than CS.

Antimicrobial and Antioxidant Activity of Chitosan/Hydroxypropyl Methylcellulose Film-Forming Hydrosols Hydrolyzed by Cellulase

“…It was noticed that samples of C0.1 T 20 statistically have the highest antioxidant power. A better antioxidant power result can be achieved by fractionation of hydrolyzed polysaccharides, as was proposed by Zimoch-Korzycka et al [ 15 ]. They have proven that original chitosan used in the degradation process possess a good reducing capacity of 447.5 and 536.6 µM Fe(II)/mL, but was still worse than their insoluble fractions, ranging from 500 to 1350 µM Fe(II)/mL.…”

“…The mode of action of high molecular weight chitosan and large particles relies on the interaction with the cell surface (resulting in changes in cell permeability [ 12 ]), or forming an impermeable layer around the cell (which blocks the transport of essential substances into the cell [ 13 ]). Many authors have proven better biological activity of chitooligomers than chitosan [ 14 , 15 , 16 ].…”

The use of chitosan, lysozyme, and the nano-silver as antimicrobial ingredients of edible protective hydrosols applied into the surface of meat

“…and E. coli with a decreasing viscosity of 1000 to 10 cP (Cho et al 1998). Chitosan oligomers obtained after lysozyme degradation exhibit stronger antilisterial activity than not hydrolyzed chitosan (Zimoch-Korzycka et al 2014). According to Wu, the increasing concentration of chitosan in membrane with the addition of cellulose also greatly reduces the growth of E. coli (Wu et al 2004).…”

Modification Effect of Cellulase on the Physicochemical Characteristic of Polysaccharides Edible Films

“…The mechanical properties and permeability of chitosan films can be controlled by selecting molecular weight and a suitable solvent system and adding plasticizer and dispersing or homogenizing agents [7]. Many researchers have proved better bactericidal effect of chitooligomers than chitin and chitosan [8,9]. Enzymatic hydrolysis of chitosan is an environmentally friendly way to obtain chitooligomers with modified properties.…”