Steroidogenic enzymes mRNA expression profile and steroids production in bovine theca cells cultured in vitro and stimulated with angiotensin II

Rigo, Melânia Lazzari; Dau, Andressa Minussi Pereira; Glanzner, Werner Giehl; Martins, Mário Rino; Zanella, Renato; Rizzetti, Tiele Medianeira; Comim, Fábio Vasconcellos; Gonçalves, Paulo Bayard Dias

doi:10.1590/0103-8478cr20240254

Cited by 2 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most likely, in case of OA treatment E2 reduction could be largely due to the reduced transcription of HSD3B1 and CYP19A1 , whereas higher levels of E2 in PA and SA treated GC can be attributed to upregulation of CYP19A1 (47). Nevertheless, a clear effect of reduced transcription of STAR that is considered as the rate-limiting factor of steroid hormone synthesis (48) might be obscured by the presence of androstenedione that is a preferred substrate for CYP19A1 to produce E2 in GC (49) in our cell culture medium.…”

Section: Discussionmentioning

confidence: 99%

Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance

Sharma

Baddela

Becker

et al. 2019

Endocrine Connections

View full text Add to dashboard Cite

High-yielding dairy cows postpartum face the challenge of negative energy balance leading to elevated free fatty acids levels in the serum and follicular fluid thus affecting the ovarian function. Here, we investigated effects of physiological concentrations of palmitic acid (PA), stearic acid (SA) and oleic acid (OA) on the viability, steroid production and gene expression in a bovine granulosa cell (GC) culture model. Treatment with individual and combined fatty acids increased the CD36 gene expression, while no significant apoptotic effects were observed. Both PA and SA significantly upregulated the expression of FSHR, LHCGR, CYP19A1, HSD3B1, CCND2 and increased 17β-estradiol (E2) production, while OA downregulated the expression of these genes and reduced E2. Interestingly, STAR was equally downregulated by all fatty acids and combination treatment. E2 was significantly reduced after combination treatment. To validate the effects of OA, in vivo growing dominant follicles (10–19 mm) were injected with bovine serum albumin (BSA) with/without conjugated OA. The follicular fluid was recovered 48 h post injection. As in our in vitro model, OA significantly reduced intrafollicular E2 concentrations. In addition, expression of CD36 was significantly up- and that of CYP19A1 and STAR significantly downregulated in antral GC recovered from aspirated follicles. The ovulation rates of OA-injected follicles tended to be reduced. Our results indicate that elevated free fatty acid concentrations specifically target functional key genes in GC both in vitro and in vivo. Suggestively, this could be a possible mechanism through which elevated free fatty acids affect folliculogenesis in dairy cows postpartum.

show abstract

Section: Discussionmentioning

confidence: 99%

Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance

Sharma

Baddela

Becker

et al. 2019

Endocrine Connections

View full text Add to dashboard Cite

show abstract

The impact of postnatal leuprolide acetate treatment on reproductive characteristics in a rodent model of polycystic ovary syndrome

Mujica

Bertolin

Bridi

et al. 2017

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Steroidogenic enzymes mRNA expression profile and steroids production in bovine theca cells cultured in vitro and stimulated with angiotensin II

Abstract: The main objective of this study was to detect the steroidogenic effects of Ang II in bovine theca cells in vitro. Bovine theca cells were obtained from follicles (larger than 10mm of diameter) collected from a local abattoir and submitted to

Cited by 2 publications

References 12 publications

Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance

Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance

The impact of postnatal leuprolide acetate treatment on reproductive characteristics in a rodent model of polycystic ovary syndrome

Contact Info

Product

Resources

About