Starch synthesis and gelatinization properties of potato tubers

Su, Wang; Ye, Guangji; Zhou, Yun; Wang, Jian

doi:10.1590/0103-8478cr20210050

Cited by 2 publications

(5 citation statements)

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“…An increase in starch content accompanied by an increase in the expression of the AGPase gene was previously recorded in potato by Müller-Röber et al [ 50 , 51 ] and Stark et al [ 50 , 51 ]. The synchronization between the increase in starch content and the expressions of the AGPase, SS, GBSS and SBE genes has been recorded in potato [ 8 ], rice [ 52 ], wheat [ 53 ] and lanzhou lily bulb [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…AGPase catalyzes the key step in starch biosynthesis; it produces ADP glucose, which provides the glycosyl moiety required for starch biosynthesis [ 7 , 8 ]. Thus, AGPase is the rate-limiting enzyme in starch accumulation in potato [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Both GBSS and SSs are responsible for chain elongation by catalyzing the formation α−1,4 glucosidic bonds in amylose and amylopectin, respectively, while SBEs are responsible for the formation of α−1.6 linkages at the branch points of amylopectin [ 6 ]. Several SSs have been characterized in plants; however, they are mainly referred to as SS II and SS III in potato [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…One μg of the purified RNA was converted to cDNA using a Sensi-FAST™ cDNA synthesis kit following the standard protocol from the manufacturer. Quantitative real-time PCR for AGPase, GBSS I, SBE I, SBE II, SS II and SS III cDNAs was carried out on a Mx3000P (Stratagene, CA, USA) qPCR system using specific primers ( Table 1 ) [ 8 ]. The thermal profile of the real-time system was 95 °C for 10 min, followed by 40 cycles (95 °C for 15 s and 60 °C for 60 s).…”

Section: Methodsmentioning

confidence: 99%

“…Starch synthesis is hosted in amyloplasts and catalyzed with granular-bound starch synthase (GBSS), specialized for elongation of growing α−1,4 linkage with exclusive or nearly exclusive activity in the soluble phase, and both starch branching enzyme (SBE) and starch synthases (SSs) to construct the branched chains [ 6 ]. The glycosyl moieties required for both amylose and amylopectin are provided as ADP glucose synthesized under catalysis of ADP glucose pyrophosphorylase (AGPase) [ 7 , 8 ]. The increased rate of starch biosynthesis during the early stages of tuber induction and the subsequent developmental stages of tuberization is underlined…”

Section: Introductionmentioning

confidence: 99%

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Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

Adly

Niedbała

El-Denary

et al. 2023

Plants

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Starch content is one of the major quality criteria targeted by potato breeding programs. Traditional potato breeding is a laborious duty due to the tetraploid nature and immense heterozygosity of potato genomes. In addition, screening for functional genetic variations in wild relatives is slow and strenuous. Moreover, genetic diversity, which is the raw material for breeding programs, is limited due to vegetative propagation used in the potato industry. Somaclonal variation provides a time-efficient tool to breeders for obtaining genetic variability, which is essential for breeding programs, at a reasonable cost and independent of sophisticated technology. The present investigation aimed to create potato somaclones with an improved potential for starch accumulation. Based on the weight and starch content of tubers, the somaclonal variant Ros 119, among 105 callus-sourced clones, recorded a higher tuberization potential than the parent cv Lady Rosetta in a field experiment. Although this somaclone was similar to the parent in the number of tubers produced, it exhibited tubers with 42 and 61% higher fresh and dry weights, respectively. Additionally, this clone recorded 10 and 75% increases in starch content based on the dry weight and average content per plant, respectively. The enhanced starch accumulation was associated with the upregulation of six starch-synthesis-related genes, namely, the AGPase, GBSS I, SBE I, SBE II, SS II and SS III genes. AGPase affords the glycosyl moieties required for the synthesis of amylose and amylopectin. GBSS is required for amylose elongation, while SBE I, SBE II, SS II and SS III are responsible for amylopectin.

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