Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry

Dutra, Tatiane Viana; Prado, Daniela Biral do; Anjos, Márcia Maria dos; Machinski, Miguel; Mikcha, Jane Martha Graton; Filho, Benício Alves de Abreu

doi:10.1590/0103-8478cr20190790

Cited by 2 publications

(2 citation statements)

References 13 publications

(13 reference statements)

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“…The proportion of 1 mL milk or broth, 100 µL of bacterial suspension and 100 µL of 300 µg/mL Sf solution and irradiation for 30 minutes was used (Figure 1A). To verify the effect of photoinactivation on bacterial biofilm removal, in each well, a stainless-steel coupon (AISI 304, simulating the surface of equipment used in the dairy industry) (8 mm x 8 mm x 1 mm) was added (Dutra et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Safranin-O-Mediated Photodynamic Inactivation of Microorganisms Reduces Milk Contaminating Bacteria Count and Prevented New Cases of Mastitis

Rodrigues¹,

Saraiva²,

Silva³

et al. 2023

Trop. Anim. Sci. J.

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The objectives of this study were to evaluate the antimicrobial capacity of the photosensitizer (PS) safranin-O (Sf), through antimicrobial photodynamic therapy (PDTa), against strains of Pseudomonas fluorescens (PV1, PVh, and PC3) and Staphylococcus aureus (SV1, SV3, SV5, SC5, SC6, and SC8) isolated from milk from cows and goats and the biofilm formation on equipment. To evaluate the effectiveness of the formulation, studies were carried out through in situ applications of the Sf hydrogel as post-dipping in lactating cows and goats. Antibiogram tests showed the resistance of Staphylococcus aureus isolates to ampicillin, trimethoprim, and tetracycline. In the in vitro assays in bovine milk, the PDTa reduced the P. fluorescens (PV1) counts by 17.4% (p<0.05). In goat milk, the PDTa reduced the growth of S. aureus by up to 24.43% (SC8) (p<0.05), and for P. fluorescens (PC3), the inactivation was 50.25%. In biofilm formation, there were reduction of 66.66% (SV1) and 38.27% (SC5) in the adhesion of S. aureus to stainless steel coupons. For in situ applications, photoactivated Sf maintained bacterial counts in bovine milk similar to the control treatment (lactic acid). During the experimental period (28 days), there were reductions of 23.23% and 28.85% in staphylococci and Pseudomonas spp. in cow's milk and 76.13% for Pseudomonas spp. in goat milk (p<0.05). There was no significant difference in goat milk's somatic cell count (CCS). These results indicate that photoactivated Sf can help reduce milk contamination and maintain the health of the mammary gland.

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Section: Methodsmentioning

confidence: 99%

Safranin-O-Mediated Photodynamic Inactivation of Microorganisms Reduces Milk Contaminating Bacteria Count and Prevented New Cases of Mastitis

Rodrigues¹,

Saraiva²,

Silva³

et al. 2023

Trop. Anim. Sci. J.

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“…Milk samples were evaluated at time 0 and after 48 h at 7 °C, and the stainless-steel coupons were evaluated after 48 h at 7 °C and 35 °C. The number of cells adhered to the stainless-steel coupons was determined according to Dutra et al (2020).…”

Section: Photoinactivation Procedures Of Experimentally Contaminated ...mentioning

confidence: 99%

Safranine-O Incorporated in F127 Nanocarriers Reduces the Contamination of Staphylococcus aureus in Sheep’s Milk

Rodrigues

Silva

Saraiva

et al. 2022

Trop. Anim. Sci. J.

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Mastitis caused byStaphylococcus aureus uses antibiotics as conventional treatment, a practice that has led to bacterial resistance. Therefore, the photodynamic inactivation of microorganisms (PDIM), has the advantage of inactivating pathogens without leading to the selection of resistant microorganisms. The objective of the study was to evaluate the antimicrobial activity of the photosensitizer (PS) Safranine-O (Sf), incorporated in the nanocarrier Pluronic ® F127 against strains of S. aureus isolated from sheep milk. The reduction of contamination in experimentally contaminated milk and Muller Hilton Broth (MHB), the formation of microbial biofilm, and its effect as post dipping in the decrease of total mesophilic aerobic and Staphylococcal counts in milk were evaluated. Three strains of S. aureus (SO1, SO3, SO4) and a coagulase-negative Staphylococcus (CNS) strain (SO2) were identified through the nuc and coa genes and all were sensitive to PDIM. The minimum inhibitory concentration (MIC) ranged from 1.18 to 18.75 mg/mL in the different isolates. The SO4 strain was resistant to Ampicillin and Trimetropim. When the microorganisms were cultivated in milk and MHB, there was a reduction in staph counts by 97.33% and 99.63%, respectively. In stainless steel coupons, photoinactivation reduced S. aureus adhesion by up to 45.92% (milk) and 99.5% (MHB) (p<0.05). The photoactivated Sf was similar to commercial lactic acid when applied as a dipping powder. These results showed that Sf mediated PDIM effectively inactivated pathogens that cause mastitis and reduced milk contamination.

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Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry

Cited by 2 publications

References 13 publications

Safranin-O-Mediated Photodynamic Inactivation of Microorganisms Reduces Milk Contaminating Bacteria Count and Prevented New Cases of Mastitis

Safranin-O-Mediated Photodynamic Inactivation of Microorganisms Reduces Milk Contaminating Bacteria Count and Prevented New Cases of Mastitis

Safranine-O Incorporated in F127 Nanocarriers Reduces the Contamination of Staphylococcus aureus in Sheep’s Milk

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