Concentrations of cytokinin and the use of agricultural residues in the in vitro propagation media of highbush blueberry

Abstract

The objective of the research was to evaluate cytokinin concentrations and agricultural residues as physical structuring agents of the culture medium in the in vitro propagation of highbush blueberry (Vaccinium corymbosum ‘Duke’). To that end, three different concentrations of 2-isopentenyladenine (2iP) (0, 5, and 10 mg L-1)in woody plant medium (WPM) and five structuring agents (in natura rice husks, carbonized rice husks, coconut fiber, S-10 Beifort®, and agar)were tested. After 60days of culture, the following parameters were evaluated: survival (%),sprout and shoot lengths (cm),propagation rate, number of sprouts, and shoot dry mass (g). The substrates used as structuring agents were analyzed in terms of pH and electrical conductivity. The in natura rice husk, S-10 Beifort®, and carbonized rice husk did not differ from the agar in terms of the sprout length. The shoots were longer (1.64 cm) in cultures within natura rice husk than in other treatments. In the absence of 2iP, S-10 Beifort® resulted in the highest propagation rate (2.97). Concerning the number of sprouts, S-10 Beifort®, in natura rice husks, and carbonized rice husks did not differ from agar. However, when the regulator was added, the agar performed better in comparison to the other residues. Thus, in natura rice husks, carbonized rice husks, and S-10 Beifort®are potential physical structuring agents of the culture medium that can be used without 2iP.

Grape-based residue as a substrate in Oncidium baueri Lindl. acclimatization

“…Furthermore, to these materials, the use of commercial substrates, such as S-10 Beifort ® , can be an option for plant acclimatization. This substrate has grapevine residues (Vitis sp) in its composition and was tested for the germination of papaya (Carica papaya) seeds (Almeida et al, 2020), pearl millet (Pennisetum glaucum) seeds (Suñe et al 2019), and micropropagation of blueberry (Vaccinium corymbosum) (Nadal et al, 2020). However, considering the scarcity of information about its use in the acclimatization of orchids, this study aimed to evaluate the use of S-10 Beifort ® , alone or in mixture with other agricultural residues, in the acclimatization of Oncidium baueri Lindl.…”