Creolin® administered by different pathways in rats experimentally poisoned with Bothrops jararaca venom

Carvalho, Lucas Rannier Ribeiro Antonino; Pereira, Helder Camilo da Silva; Silva, Hugo Thyares Fonseca Nascimento Pereira da; Lucena, Ricardo Barbosa; Guerra, Ricardo Romão

doi:10.1590/0103-8478cr20180699

Cited by 1 publication

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“…In the case of death due to senescence or euthanasia (see section 2.2 ), necropsy was performed, with macroscopic analysis, photo documentation, and collection of organ samples for histopathological evaluation(s). The samples were immediately stored in a 10% formalin solution for fixation, and later preparation of slides as previously described [ 34 ]. The tissues were dehydrated using ethyl alcohol in increasing concentrations, diaffinized in xylene, embedded in liquid paraffin (60 °C), and cut using a microtome (5 μm).…”

Section: Methodsmentioning

confidence: 99%

Effects of chronic dietary nitrate supplementation on longevity, vascular function and cancer incidence in rats

et al. 2021

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Rationale Dietary nitrate and nitrite have a notoriously bad reputation because of their proposed association with disease, in particular cancer. However, more recent lines of research have challenged this dogma suggesting that intake of these anions also possess beneficial effects after in vivo conversion to the vital signaling molecule nitric oxide. Such effects include improvement in cardiovascular, renal and metabolic function, which is partly mediated via reduction of oxidative stress. A recent study even indicates that low dose of dietary nitrite extends life span in fruit flies. Methods In this study, 200 middle-aged Wistar rats of both sexes were supplemented with nitrate or placebo in the drinking water throughout their remaining life and we studied longevity, biochemical markers of disease, vascular reactivity along with careful determination of the cause of death. Results Dietary nitrate did not affect life span or the age-dependent changes in markers of oxidative stress, kidney and liver function, or lipid profile. Ex vivo examination of vascular function, however, showed improvements in endothelial function in rats treated with nitrate. Neoplasms were not more common in the nitrate group. Conclusion We conclude that chronic treatment with dietary nitrate does not affect life span in rats nor does it increase the incidence of cancer. In contrast, vascular function was improved by nitrate, possibly suggesting an increase in health span.

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Section: Methodsmentioning

confidence: 99%