Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

Pereira, Daniele Araujo; Vidotto, Marilda Carlos; Nascimento, Karla Alvarenga; Santos, Adriana Cavalcanti dos; Mechler, Marina Lopes; Oliveira, Luís Guilherme de

doi:10.1590/0103-8478cr20151269

Cited by 14 publications

(15 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, E. coli vaccines have been used for many years to stimulate mucosal immunity of the sows and, hence, to elicit specific IgG and IgA antibodies in colostrum, conferring protection to the neonatal piglets [5][6][7]. However, the available parenteral vaccines to prevent postweaning diarrhea tend to stimulate the systemic rather than mucosal immune system [8][9][10][11][12][13]. In contrast to parenteral vaccines, mucosal vaccines are safer and induce immunity both systemically as well as in mucosal tissues.…”

Section: Introductionmentioning

confidence: 99%

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Matías

Brotons

Cenoz³

et al. 2019

Vaccines

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of illness and death in neonatal and recently weaned pigs. The immune protection of the piglets derives from maternal colostrum, since this species does not receive maternal antibodies through the placenta. In the present study, outer membrane vesicles (OMVs) obtained from main ETEC strains involved in piglet infection (F4 and F18 serotypes), encapsulated into zein nanoparticles coated with Gantrez®® AN-mannosamine conjugate, were used to orally immunize mice and pregnant sows. Loaded nanoparticles were homogeneous and spherical in a shape, with a size of 220–280 nm. The diffusion of nanoparticles through porcine intestinal mucus barrier was assessed by a Multiple Particle Tracking technique, showing that these particles were able to diffuse efficiently (1.3% diffusion coefficient), validating their oral use. BALB/c mice were either orally immunized with free OMVs or encapsulated into nanoparticles (100 µg OMVs/mouse). Results indicated that a single dose of loaded nanoparticles was able to elicit higher levels of serum specific IgG1, IgG2a and IgA, as well as intestinal IgA, with respect to the free antigens. In addition, nanoparticles induced an increase in levels of IL-2, IL-4 and IFN-γ with respect to the administration of free OMVs. Orally immunized pregnant sows with the same formulation elicited colostrum-, serum- (IgG, IgA or IgM) and fecal- (IgA) specific antibodies and, what is most relevant, offspring suckling piglets presented specific IgG in serum. Further studies are needed to determine the infection protective capacity of this new oral subunit vaccine

show abstract

Section: Introductionmentioning

confidence: 99%

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Matías

Brotons

Cenoz³

et al. 2019

Vaccines

View full text Add to dashboard Cite

show abstract

“…At the same time, diarrhoea causes serious economic losses in pig production due to increased morbidity, mortality, treatment costs and poor growth (9,12,18). Escherichia coli (E. coli) live in the intestines and are part of the commensal microbiota that plays an important role in the protection of the intestinal tract of animals (21,26). Nevertheless, there are E. coli strains which cause dangerous infections in suckling and/or weaned piglets.…”

Section: Praca Oryginalnamentioning

confidence: 99%

“…The initial step in the pathogenesis of disease is the attachment of the ETEC strains to the microvilli of the small intestine epithelial cells (17,18,26). Adhesins target specific receptors on the intestinal epithelial cells, thus enabling the transfer of enterotoxins on target cells (7,16,21). Two enterotoxin classes are well known: the high molecular weight heat-labile toxin (LT) and low molecular weight heat-stable a and b toxins (STa and STb) (6,12,16).…”

Section: Praca Oryginalnamentioning

confidence: 99%

See 1 more Smart Citation

Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia

Žutić¹,

Valčić²,

Milićević³

et al. 2020

Medycyna Weterynaryjna

View full text Add to dashboard Cite

A total of 120 Escherichia coli (E. coli) strains from suckling piglets with diarrhoea and 30 E. coli strains from healthy piglets were tested for the presence of fimbrial and enterotoxin virulence genes. Out of the 120 isolates sampled from diarrheic piglets, 81 (67.5%) expressed one or more genes encoding virulence factors. Adhesin genes were detected in 52 (43.33%) out of 120 E. coli isolates, and the most common among them was F4 adhesin (33.33%). Genes encoding E. coli toxins were detected in 81 (67.5%) isolates. E. coli included in the study carried genes for one or more of the following toxins: STa, STb, LT and EAST1. The astA gene encoding EAST1 was the most prevalent and was identified in 72 (60%) E. coli isolates. EAST1 toxin was detected in 5 out of 30 isolates (16.7%) from healthy piglets. Among the 81 isolates expressing virulence genes, a total of 15 different combinations for fimbrial and toxin genes were found. The most common virulence pattern was F4/STb/LT/EAST1 detected in 23.45% of E. coli strains isolated from suckling piglets with diarrhoea. The results indicate that F4 adhesin and EAST1 toxin are the most common in E. coli isolates sampled from diarrhoeic suckling piglets in Serbia.

show abstract

“…Attachment to human cells leading to gut colonisation [17] may be primary in mediating enteric disorders and are related with adhesins on fimbriae F4 (K88), F5 (K99), F6 (987P), F41, and F18 [18]. Hemagglutinins are also known E. coli cell surface virulence factors carried by fimbriae.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Characterisation of Escherichia coli Isolates from Fish, Diarrheic and Healthy Children in Zanzibar, Tanzania

Rabia

Wambura

Kimera

et al. 2017

IJTDH

View full text Add to dashboard Cite

and fish consumers through the seasons. Cross sectional design was used to collect children faecal samples from Mnazimmoja referral hospital. Place and Duration of Study: The study was carried out in Zanzibar between August 2014 and June 2016 Methodology: A total of 113 E. coli isolates from fish (58), diarrheic (35) and healthy children (20) less than five years old were screened. Serotyping was used for detection of serotype O157 and extended-spectrum-β-lactamases (ESBL) production by disc diffusion using Cepodoxime/clavulanic acid (10/1μg) discs. Hemolysin was detected by hemolysis in human blood agar, serum resistance factors by growth inhibition of E. coli that was mixed and incubated with human sera, Hemagglutinins by Hemagglutination of RBCs and Gelatinase enzyme by production of clear zone of degradation of gelatine. Statistical analysis was done using Medcalc statistical software. Statistical difference in virulence factors possession data in E. coli isolates between fish, diarrheic and healthy children were subjected to analysis of variance [ANOVA], where p<0.05 was judged indicative of significant difference. Results: Virulence factors detected in their order of prevalence were hemolysins (21.2%), serum resistance (12.2%), hydrophobicity (8.8%), Hemagglutinins (4.4%), and Gelatinase (2.7%). Virulence factors were detected in 82.9% and 38% of diarrheic children and fish isolates respectively. E. coli O157 serotype was detected in all 3 sources with higher percentage in diarrheic children (6.8%). Extended spectrum β-lactamase E. coli producers were found in fish (6.8%) and diarrheic samples (17.1%) but not from healthy children. Multidrug resistance [MDR] was detected in fish (44.9%), diarrheic children (82.8%) and healthy children (10%). Ampicillin (100%; 22.4%) and Tetracycline (82.9%; 44.8%) exhibited high antibiotic resistance both in diarrheic children and fish respectively. Fish foods could be sources of pathogenic and antibiotic resistant E. coli possessing multiple virulence factors. Moreover O157 serotypes, ESBL producing and multidrug resistant E. coli were also detected in fish foods. Conclusion: It is therefore emphasised to improve hygiene in the fish value chain as well as raise awareness and frequent monitoring to fish stakeholders in Zanzibar.

show abstract

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

Cited by 14 publications

References 40 publications

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Oral Immunogenicity in Mice and Sows of Enterotoxigenic Escherichia Coli Outer-Membrane Vesicles Incorporated into Zein-Based Nanoparticles

Profiles of virulence genes in enterotoxigenic Escherichia coli isolates from suckling piglets in Serbia

Phenotypic Characterisation of Escherichia coli Isolates from Fish, Diarrheic and Healthy Children in Zanzibar, Tanzania

Contact Info

Product

Resources

About