Safety of the novel influenza viral vector Brucella abortus vaccine in pregnant heifers

Tabynov, Kaissar; Ryskeldinova, Sholpan; Kydyrbayev, Zhailaubay; Sansyzbay, Abylai

doi:10.1590/0103-8478cr20150497

Cited by 9 publications

(13 citation statements)

References 8 publications

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“…Despite the fact that undulant fever is one of the major symptoms of Brucella infection in humans [37], [38], the induction of fever associated with infection or vaccination has never been investigated in swine. In a recent study, an increase in body temperature was observed in pregnant heifers within 1 to 2 days following vaccination with Brucella abortus S19 [39]. To the best of our knowledge, this is the first study investigating body temperature fluctuations as a potential side effect of vaccination in swine.…”

Section: Discussionmentioning

confidence: 71%

Vaccine safety studies of Brucella abortus S19 and S19ΔvjbR in pregnant swine

et al. 2019

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HighlightsVaccination with Brucella abortus S19 or S19ΔvjbR in pregnant swine did not induce abortion, stillbirths or a reduction in litter size.Gross and histopathological evaluation did not demonstrate any local or systemic side effect associated with either vaccine.At the time of the delivery, there was no evidence of the presence of either vaccine strains in the fetuses, placentas or sows.Both vaccine candidates are safe for use in pregnant swine.

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Section: Discussionmentioning

confidence: 71%

Vaccine safety studies of Brucella abortus S19 and S19ΔvjbR in pregnant swine

et al. 2019

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“…abortus ) we developed Flu-BA vaccine candidate in which a vector based on recombinant influenza virus subtypes H5N1 and H1N1 expresses the Brucella L7/L12 or Omp16 proteins from the NS1 (non-structural) gene open reading frame (ORF). In the past few years, we conducted many vaccine trials and demonstrated this vaccine’s safety and efficacy [6, 7]. Our results were comparable or even superior in vaccine trials comparing it to commercial vaccines made from B .…”

Section: Introductionmentioning

confidence: 65%

“…abortus S19 and RB51 in cattle, including in pregnant heifers [8, 9]. We also demonstrated our candidate vaccine’s ability to induce prolonged protective immune response for up to 12 months [10], as well as showed its DIVA potential [7]. Furthermore, as the bovine Flu-BA vaccine is genetically stable, the vaccine virus does not excrete from the body into the environment, provides cross-protection against B .…”

Section: Introductionmentioning

confidence: 98%

Improved influenza viral vector based Brucella abortus vaccine induces robust B and T-cell responses and protection against Brucella melitensis infection in pregnant sheep and goats

Mailybayeva¹,

Yespembetov²,

Ryskeldinova³

et al. 2017

PLoS ONE

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We previously developed a potent candidate vaccine against bovine brucellosis caused by Brucella abortus using the influenza viral vector expressing Brucella Omp16 and L7/L12 proteins (Flu-BA). Our success in the Flu-BA vaccine trial in cattle and results of a pilot study in non-pregnant small ruminants prompted us in the current study to test its efficacy against B. melitensis infection in pregnant sheep and goats. In this study, we improved the Flu-BA vaccine formulation and immunization method to achieve maximum efficacy and safety. The Flu-BA vaccine formulation had two additional proteins Omp19 and SOD, and administered thrice with 20% Montanide Gel01 adjuvant, simultaneously by both subcutaneous and conjunctival routes at 21 days intervals in pregnant sheep and goats. At 42 days post-vaccination (DPV) we detected antigen-specific IgG antibodies predominantly of IgG2a isotype but also IgG1, and also detected a strong lymphocyte recall response with IFN-γ production. Importantly, our candidate vaccine prevented abortion in 66.7% and 77.8% of pregnant sheep and goats, respectively. Furthermore, complete protection (absence of live B. melitensis 16M) was observed in 55.6% and 66.7% of challenged sheep and goats, and 72.7% and 90.0% of their fetuses (lambs/yeanlings), respectively. The severity of B. melitensis 16M infection in vaccinated sheep and goats and their fetuses (index of infection and rates of Brucella colonization in tissues) was significantly lower than in control groups. None of the protection parameters after vaccination with Flu-BA vaccine were statistically inferior to protection seen with the commercial B. melitensis Rev.1 vaccine (protection against abortion and vaccination efficacy, alpha = 0.18–0.34, infection index, P = 0.37–0.77, Brucella colonization, P = 0.16 to P > 0.99). In conclusion, our improved Flu-BA vaccine formulation and delivery method were found safe and effective in protecting pregnant sheep and goats against adverse consequences of B. melitensis infection.

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“…Animals, mostly those vaccinated as adults, show persistent serological reactions against the antigenic O-chain of the lipopolysaccharide of the smooth Brucella strains [ 55 ]. In addition, a recent study showed that pregnant heifers vaccinated with a high dose of the B. abortus S19 vaccine displayed an increased body temperature within the first two days post vaccination [ 56 ]. Nevertheless, it was previously demonstrated that [ 57 ] the subcutaneous or conjunctival vaccination of animals with a reduced dose ranging from 3 ×10 8 to 5 × 10 9 can decrease the serological antibody response.…”

Section: Resultsmentioning

confidence: 99%

Whole Genome Sequence Analysis of Brucella abortus Isolates from Various Regions of South Africa

et al. 2021

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The availability of whole genome sequences in public databases permits genome-wide comparative studies of various bacterial species. Whole genome sequence-single nucleotide polymorphisms (WGS-SNP) analysis has been used in recent studies and allows the discrimination of various Brucella species and strains. In the present study, 13 Brucella spp. strains from cattle of various locations in provinces of South Africa were typed and discriminated. WGS-SNP analysis indicated a maximum pairwise distance ranging from 4 to 77 single nucleotide polymorphisms (SNPs) between the South African Brucella abortus virulent field strains. Moreover, it was shown that the South African B. abortus strains grouped closely to B. abortus strains from Mozambique and Zimbabwe, as well as other Eurasian countries, such as Portugal and India. WGS-SNP analysis of South African B. abortus strains demonstrated that the same genotype circulated in one farm (Farm 1), whereas another farm (Farm 2) in the same province had two different genotypes. This indicated that brucellosis in South Africa spreads within the herd on some farms, whereas the introduction of infected animals is the mode of transmission on other farms. Three B. abortus vaccine S19 strains isolated from tissue and aborted material were identical, even though they originated from different herds and regions of South Africa. This might be due to the incorrect vaccination of animals older than the recommended age of 4–8 months or might be a problem associated with vaccine production.

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Safety of the novel influenza viral vector Brucella abortus vaccine in pregnant heifers

Cited by 9 publications

References 8 publications

Vaccine safety studies of Brucella abortus S19 and S19ΔvjbR in pregnant swine

Vaccine safety studies of Brucella abortus S19 and S19ΔvjbR in pregnant swine

Improved influenza viral vector based Brucella abortus vaccine induces robust B and T-cell responses and protection against Brucella melitensis infection in pregnant sheep and goats

Whole Genome Sequence Analysis of Brucella abortus Isolates from Various Regions of South Africa

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