Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization

Vasconcelos, Carlos Otávio de Paula; Brandão, Felipe Zandonadi; Martins, Gabriel; Penna, Bruno; Souza-Fabjan, J. M. G.; Lilenbaum, Walter

doi:10.1590/0103-8478cr20150365

Cited by 18 publications

(20 citation statements)

References 14 publications

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“…The information obtained in the current study support previous reports on the drawbacks which may be associated to the use of progestogen‐based protocols to control reproduction in sheep (De Paula Vasconcelos et al., ; Lopez‐Sebastian et al., ). Despite a 100% oestrous response at the induced ovulation and a normal pattern of luteal function as assessed by progesterone secretion, lambing rate after mating at the induced oestrus was slightly over 50%, significantly lower than for “PGF” ewes.…”

Section: Discussionsupporting

confidence: 90%

GnRH and prostaglandin‐based synchronization protocols as alternatives to progestogen‐based treatments in sheep

Rekik¹,

Haile

Abebe

et al. 2016

Reprod Domestic Animals

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The study investigated, for cycling sheep, synchronizing protocols simultaneously to the standard "P" protocol using progestogens priming with intravaginal devices and gonadotropin. In November 2014, 90 adult Menz ewes were assigned to either the "P" protocol, "PGF" treatment where oestrus and ovulation were synchronized using two injections of prostaglandin 11 days apart or a "GnRH" treatment where the ewes had their oestrus and ovulation synchronized with GnRH (day 0)-prostaglandin (day 6)-GnRH (day 9) sequence. The ewes were naturally mated at the induced oestrus and the following 36 days. Plasma progesterone revealed that 92% of the ewes were ovulating before synchronization and all, except one, ovulated in response to the applied treatments. All "P" ewes exhibited oestrus during the 96-hr period after the end of the treatments in comparison with only 79.3% and 73.3% for "PGF" and "GnRH" ewes, respectively (p < .05). Onset and duration of oestrus were affected by the hormonal treatment (p < .05); "GnRH" ewes showed oestrus earliest and had the shortest oestrous duration. Lambing rate from mating at the induced oestrus was lower for "P" than for "PGF" ewes (55.6% and 79.3%, respectively; p < .05). The same trait was also lower for "P" than for "PGF" and "GnRH" ewes (70.4%, 89.7% and 86.7%, respectively; p < .05) following the 36-day mating period. Prostaglandin and GnRH analogue-based protocols are promising alternatives for both controlled natural mating and fixed insemination of Menz sheep after the rainy season when most animals are spontaneously cycling.

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Section: Discussionsupporting

confidence: 90%

GnRH and prostaglandin‐based synchronization protocols as alternatives to progestogen‐based treatments in sheep

Rekik¹,

Haile

Abebe

et al. 2016

Reprod Domestic Animals

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“…On the other hand, number of vaginal bacteria decreased to values before sponge was applied as progesterone applied ewes showed estrus when sponges were removed, and no decrease occurred in the number of vaginal bacteria in ewes to which empty sponge was attached as no estrus developed after sponges were removed. However, Vasconcelos et al (2016) identified in a study they carried out on ewes that although vaginitis was observed in all MAP and CIDR applied ewes including the control group after vaginal devices were removed, that CFU analysis of the control group was lower than the ewes to which MAP and CIDR were applied. This result shows that unlike the findings of Yeşilmen et al (2008), hormones -synthetic or regardless of their natural origin -also increase bacterial load as well as physical movement of…”

Section: Factors Leading To Change In Vaginal Microbiotamentioning

confidence: 99%

Importance of Characterization of the Vaginal Microbiota in Ewes and Nannies

Altinçekiç

Koyuncu

2018

Hayvansal Üretim

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The referees list / Hakem listesi Journal of Animal Production is a peer-reviewed journal. List of referees is given in the last press issue of the year.Hayvansal Üretim hakemli bir dergi olup, hakem listesi her yılın son sayısında basılı yayınlanmaktadır.Journal of Animal Production is published two times in a year (May and November) by Ege Animal Science Association in Turkey. Detail information about Ege Animal Science Association and Journal of Animal Science could be finding from the web site of the Ege Animal Science Association or correspondence address of the journal given below. Guidelines to authors are also given at the end of each issue of the journal.Hayvansal Üretim dergisi, Ege Zootekni Derneği'nin "yaygın süreli" bir yayınıdır. Yılda iki kez (Mayıs ve Kasım aylarında) yayınlanmaktadır. Ege Zootekni Derneği ve Hayvansal Üretim dergisine ilişkin ayrıntılı ve güncel bilgiler Ege Zootekni Derneği'nin internet sitesinden veya dergi yazışma adresinden öğrenilebilir. Yazım kuralları derginin her sayısının sonunda verilmektedir.

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“…Al-Hamedawi et al (2003) stated some changes in the number of vaginal bacteria as a result of the physical actions exerted by the devices that retained and absorbed vaginal secretions throughout their stay in the vagina. Vasconcelos et al (2016), reported that vaginitis six days after the intravaginal devices were inserted, with typical clinical signs such as mucopurulent discharge, erythemas and increased local sensitivity, besides a considerable increase in bacterial count, independently of the type of device and the presence of progesterone. According to Vasconcelos et al (2016), the agents of the vaginitis were coliforms, which were replaced by the original microbiota 48 hours after implant was removed.…”

Section: Bacterial Countmentioning

confidence: 99%

“…Vasconcelos et al (2016), reported that vaginitis six days after the intravaginal devices were inserted, with typical clinical signs such as mucopurulent discharge, erythemas and increased local sensitivity, besides a considerable increase in bacterial count, independently of the type of device and the presence of progesterone. According to Vasconcelos et al (2016), the agents of the vaginitis were coliforms, which were replaced by the original microbiota 48 hours after implant was removed. However, researchers (Suarez et al, 2006;Yesilmen et al, 2008;Oliveira et al, 2013) have reported that CFUs number increased with the device's insertion but decreased after the device's withdrawal.…”

Section: Bacterial Countmentioning

confidence: 99%

Investigation of the Effect of Intravaginal Device-type and Treatment-duration on Vaginal Features in Ewes and Determination of Antibiotic Susceptibility

Altýncekic

Cilo

Duru

et al. 2020

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Background: Seasonal anestrus, which is controlled by the photoperiod in ewes, limits the ability to the fertility of ewe every time. Therefore, using hormones by vaginal devices is the most effective way of increasing fertility in order to form oestrus during the anestrus period and obtain a high rate of pregnancy. But, vaginal devices may cause a change in the profile and number of bacteria in the uterus and vagina, and thus create an environment suitable for infection. For this reason, identification of bacteria that are the source of infection can be achieved by performing antibiotic susceptibility tests and interpreting them correctly. The purpose of this study was to characterize the effect of intravaginal device-type and treatment-duration on vaginal bacteria count and type and determine the antibiotic susceptibility of vaginal bacterias in ewes after a program of oestrus synchronization. Methods: A total of 90 clinically healthy Kivircik ewes were selected. In order to provide oestrus synchronization during anestrus season (June), CIDR was inserted intravaginally to 45 ewes, the other 45 ewes were progestogen sponges inserted. In both groups remained inserted for 5, 9 and 13 days. While vaginal devices were withdrawal both groups ewes were exposed to intramuscular 350 IU PMSG injection. The mucous samples were collected from all ewes 0, 5, 9 and 13 days at time the IVS or CIDR withdrawal. In samples, bacterial concentration, bacterial identification, and antibiotic susceptibility were determined. Result: The changes in the vaginal flora caused by the vaginal devices favored the development of opportunistic microbiota that could potentially be pathogenic. In the study, after vaginal device withdrawal, the bacteria species most frequently observed in both IVS and CIDR groups were E. coli. According to the results of this study, the first choice of antibiotics for vaginal infections caused by G- agents in ewes should be any of AXC, CEP and CTZ compared to other antibiotics.

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Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization

Abstract: This study evaluated the presence of vaginitis and the bacterial load associated with different intravaginal implants in ewes. Twenty-four Dorper and crossbred ewes were allocated into three groups and received intravaginal implant containing 0.3g progesterone (CIDR ®)

Cited by 18 publications

References 14 publications

GnRH and prostaglandin‐based synchronization protocols as alternatives to progestogen‐based treatments in sheep

GnRH and prostaglandin‐based synchronization protocols as alternatives to progestogen‐based treatments in sheep

Importance of Characterization of the Vaginal Microbiota in Ewes and Nannies

Investigation of the Effect of Intravaginal Device-type and Treatment-duration on Vaginal Features in Ewes and Determination of Antibiotic Susceptibility

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