In vitro development of Encyclea cordigera in different concentrations of honey

Mantovani, Cibele; Pivetta, Káthia Fernandes Lopes

doi:10.1590/0103-8478cr20150046

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Honey is a natural source of sugars, enzymes, amino acids, organic acids, vitamins, and minerals. A beneficial effect of honey as a medium additive was earlier reported for callus induction and plant regeneration in Miscanthus × giganteus (Płażek and Dubert 2010), and also for in vitro plantlet formation in an orchid, Encyclea cordigera (Mantovani and Fernandes Lopes Pivetta 2016).…”

Section: Introductionmentioning

confidence: 58%

Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench

Dreger

Mól

Deja

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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Sorghum bicolor is a recalcitrant species for tissue culture regeneration and genetic transformation. Browning of explants is one of the factors limiting organ and tissue cultures. To overcome this, callus tissue was initiated from the shoot tips of in vitro germinating seeds (S. bicolor cv. Róna 1), and then cultured on modified MS media (Murashige and Skoog in Physiol Plant 15:473-497, 1962). In the first experiment, we tested callus induction on several media supplemented with casein hydrolysate, polyvinylpyrrolidone, honey, and sucrose. The best callus induction was recorded for the medium with honey and sucrose (80.0%) and for control medium (79.8%). Shoot regeneration was tested on the MS medium with 6-benzylaminopurine (BAP) supplemented with honey and sucrose at a 1:1 ratio (by weight) or with sucrose only. The highest percentage of calluses regenerating shoots was noted for those induced on the medium with sucrose and honey-approx. four times higher when compared to the control. Rooted plantlets were acclimatized with a 92% survival rate. In the second experiment, we analyzed culture responses to various ways of honey application to the induction media: honey (autoclaved or filtered) in presence or absence of sucrose. Supplementation of the medium with fructose, glucose, and maltose at a proportion typical for honey was also investigated. The explant and callus survival rates were similar to those of the honey-sucrose combination in the first experiment. Only presence of both sucrose and honey in the induction medium improved the total regeneration rate to 37.9% over the control (18.8%). Sucrose and honey appear to act synergistically for shoot regeneration in callus cultures of sorghum.

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Section: Introductionmentioning

confidence: 58%

Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench

Dreger

Mól

Deja

et al. 2019

In Vitro Cell.Dev.Biol.-Plant

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“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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