Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Casagrande, Renata Assis; Pires, Prhiscylla Sadanã; Silva, Rodrigo; Sonne, Luciana; Borges, João Batista; Neves, Monique Silva; Rolim, Verônica Machado; Souza, Suyene Oltramari de; Driemeier, David; Lobato, Francisco Carlos Faria

doi:10.1590/0103-8478cr20141447

Cited by 15 publications

(19 citation statements)

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“…This agent is responsible for blackleg, a very common disease in cattle that occurs also in large outbreaks (LOBATO et al, 2013). Notably, in the cases where only C. chauvoei was detected, the owners reported that animals with good nutrition and in good health were affected, which also corroborates previous studies (WILLIAMS, 1977;ASSIS et al, 2010;GROSETH et al, 2011;CASAGRANDE et al, 2015).…”

Section: Resultssupporting

confidence: 89%

“…Two samples from myocardial muscle were positive for C. chauvoei alone, confirming the diagnosis of clostridial myocarditis, a manifestation of blackleg that commonly leads to sudden death in bovines (UZAL et al, 2003a;CASAGRANDE et al, 2015). In fact, among the 28 cases evaluated, eight (28.6%) reported sudden death of apparently healthy animals, including the three diagnosed with clostridial myocarditis.…”

Section: Resultsmentioning

confidence: 59%

“…Specimens are rarely submitted to the laboratory for diagnosis and confirmation (LOBATO et al, 2013). In addition, the literature is mostly based on case descriptions, commonly atypical cases or outbreak reports (LIMA et al, 2006;CASAGRANDE et al, 2015). Thus, the frequency of the clostridia species involved in regular myonecrosis is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil

et al. 2017

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

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A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil

et al. 2017

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“…In addition to classical clinical signs of black disease, a few of the sick animals showed nose bleeding which is unusual clinical signs in naturally or experimentally infected cattle. At necropsy, the pathognomic lesions of black disease were prominent and all dead animals showed hemorrhagic myositis, crepitus sounds, rancid odor and necrotizing lesions in muscles of the neck, hind and front quarters [21,22]. Severe cardiac lesions have been observed; mainly diffuse necrohemorrhagic myocarditis associated with extensive enlargement of the spleen.…”

Section: Discussionmentioning

confidence: 99%

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan

Hussain

Javed

Khan

et al. 2019

J Infect Dev Ctries

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Introduction: Clostridium chauvoei (C. chauvoei) is an anaerobic, histotoxic Gram-positive, bacterium causing fatal myonecrosis in livestock with high mortalities. The disease is common in dairy animals, but little is known about the pathophysiology of the disease in exotic (non-native) animals kept under local conditions in Pakistan. Methodology: Diagnosis of blackleg was made based on hematological and serum biochemical analysis, PCR, necropsy and histopathology. Results: Clinically sick animals exhibited fever, lameness, subcutaneous gaseous swelling and edema particularly in hindquarter and front legs. Hematological analysis showed increases in erythrocyte sedimentation rate and reduces in number of red blood cells, packed cell volume, leukocytes and differential leukocyte count. Serum aspartate aminotransferase, lactate dehydrogenase, alkaline phosphates, alanine aminotransferase, urea, creatinine, creatine kinase, and creatinine phosphokinase were significantly (P < 0.05) higher in the infected animals. At necropsy, swelling areas contained straw-colored fluid with gas bubbles. The muscles were swollen, dark to black and exhibited crepitation sounds at the time of incisions with a rancid odor. Severe pulmonary edema, myocarditis along with petechial hemorrhages, as well as enlargement and congestion of liver and spleen have been observed. Microscopic examination revealed severe inflammatory reaction, edema, and disruption of the myofibrils. Examination of heart, spleen, liver, kidneys, intestine, and lungs showed congestion, severe inflammatory changes with neutrophilic infiltration and necrosis accompanied by dissociation of the normal tissue structure. PCR confirmed C. chauvoei in exudates and different samples of muscles. Conclusion: The pathophysiology should be considered in diagnosis of blackleg. The disease is exist in the non-native cattle farms and biosecurity measures have to be elevated.

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“…Pires et al (15), in studies of pigs, and Casagrande et al (5), in studies of cattle, using the streptavidinbiotin-peroxidase technique, reported positive staining in immunohistochemical analysis. In this study, performed by similar methods, positive immunohistochemical results were obtained that supported gross, microscopical, and microbiological findings, similar to those of the researchers.…”

Section: Tab 2 Biochemical Parametersmentioning

confidence: 99%

Examination of sheep in which injection of Yeldif® induced sudden death

Uyar

Yener

Başbuğan

et al. 2017

Medycyna Weterynaryjna

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Two breeders from the Başkale district of the Van province and the Hizan district of the Bitlis province reported deaths of their lambs one day after injection of a commercial preparation containing a combination of Se and Vit E (Yeldif®). Purulent-hemorrhagic-necrotic myositis was detected at the injection site during necropsy of the lambs (2 animals) brought to the Pathology Department of our Faculty by these breeders. This study was conducted to determine whether these post-injection deaths occurred as a result of the drug used or due to a faulty application of the medicine by the breeders. The trial period for this study was 1 week. Eighteen 2-month-old lambs were used in this study. The lambs were divided into three groups (control and 2 study groups) of 6 lambs each. An injection of 1 ml (recommended dose) of the product purchased in the market from the same batch as the one that had caused the deaths was administered I.M. to lambs in the control group. The medicine reported to have caused the deaths by the breeders was administered to the first study group (A) at 1 ml I.M. and to the second study group (B) at 3 ml I.M. (three times the recommended dose). The lambs were bled for biochemical analysis before the administration, on the first day after the administration, and at the end of the trial. Necropsies were performed on dead animals during the trial and on all animals at the end of the trial. At the end of the trial, no clinical-pathological findings were noted in lambs of the control group and group A. However, in all lambs of group B, emphysematous gangrene was detected at the injection site. Cl. chauvoei was isolated and identified in bacteriological and cultural examinations. It was concluded that bacterial contamination occurred as a result of incorrect drug administration by the breeders and injection of a high dose of the contaminated drug.

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Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Cited by 15 publications

References 10 publications

A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil

A retrospective study on the diagnosis of clostridial myonecrosis in ruminants in Brazil

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan

Examination of sheep in which injection of Yeldif® induced sudden death

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