Epidemiological status of felid herpesvirus type-1 and feline calicivirus infections in Brazil

Henzel, Andréia; Lovato, Luciane Teresinha; Weiblen, Rudi

doi:10.1590/0103-8478cr20141202

Cited by 3 publications

(1 citation statement)

References 32 publications

(124 reference statements)

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“…The results showed that the prevalence of FHV-1 and FCV was 11.3 and 27.8%, respectively, and the prevalence of the two viruses was statistically significant (Table 2). This result was the same as that of previous epidemiological studies, which revealed that infections with FCV were more common (Henzel et al, 2015;Ravicini et al, 2016;Fernandez et al, 2017). In addition, the screening results using dnRT-PCR and conventional RT-PCR/PCR are consistent, and the concordance rates were 99.13% for FHV-1 and 98.26% for FCV (Table 3), respectively, suggesting that the dnRT-PCR could be used as a highly specific and sensitive method to differentiate FHV-1 and FCV in clinical samples.…”

Section: Discussionsupporting

confidence: 92%

Rapid Detection of Feline Calicivirus and Feline Herpesvirus by Duplex Nested RT-PCR

Yi¹

2018

PVJ

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The aim of this study was to develop a novel duplex nested RT-PCR (dnRT-PCR) method which can simultaneously detect and distinguish feline herpesvirus type 1 (FHV-1) and feline calicivirus (FCV) in cats with upper respiratory tract disease (URTD) and evaluate the performance of this assay in clinical screening. Four pairs of primers based on TK gene of FHV-1 and ORF2 gene of FCV were designed, respectively, and a dnRT-PCR assay was developed and evaluated. A total of 115 clinical swaps of cats collected from private veterinary clinics and animal shelters in Jilin province between November 2016 and May 2017 were used to evaluate this newly developed dnRT-PCR method compared with conventional assay. The results indicated that the detection limits of this method were 10 1.0 TCID50/ml, 10 2.2 TCID50/ml and 10 2.0 TCID50/ml for FHV-1, FCV and a mixture of the two viruses which were 100-fold and 1000-fold higher than single and duplex conventional RT-PCR/PCR, respectively, and was also highly specific. Clinical screening indicated the concordance rates for dnRT-PCR and single conventional RT-PCR/PCR were 99.13% (kappa=0.955) for FHV-1 and 98.26% (kappa=0.956) for FCV, respectively. These results suggest that the duplex nested RT-PCR is a rapid and sensitive method that is useful for clinical diagnosis and etiological investigation of feline caolicivirus and feline herpesvirus.

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Section: Discussionsupporting

confidence: 92%

Rapid Detection of Feline Calicivirus and Feline Herpesvirus by Duplex Nested RT-PCR

Yi¹

2018

PVJ

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Common viral diseases of dogs and cats

Aliyu

2024

Introduction to Diseases, Diagnosis, and Management of Dogs and Cats

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Communication of the Results of the Treatment with Probiotics in Two Cats with Chronic Gingivostomatitis

Segovia¹,

Torras²

2018

OJVM

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Feline chronic gingivostomatitis (GECF) is a disease of unknown etiology and considered as one of the most frequent paradontal diseases in cats of medium and/or advanced age. This problem is aggravated in the case of cats suffering from some immunosuppressive pathology such as feline leukemia virus (FeLV), feline immunodeficiency virus (HIV) or feline panleukopenia virus (VPF), in which the probability of suffering from GECF is increased. There is a wide variety of treatments against GECF but none of them ensures a complete cure of the disease, with signs of recurrence being observed at the month of treatment. For these reasons, in this investigation we have evaluated a strain of Lactobacillus plantarum with probiotic capacity for the preventive and/or therapeutic purpose of this oral pathology in cats. This investigation was performed in accordance with the Institutional Animal Ethics Committee of the Veterinary Faculty of the Autonomous University of Barcelona.

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Epidemiological status of felid herpesvirus type-1 and feline calicivirus infections in Brazil

Abstract: Feline calicivirus (FCV) and felid herpesvirus type-1

Cited by 3 publications

References 32 publications

Rapid Detection of Feline Calicivirus and Feline Herpesvirus by Duplex Nested RT-PCR

Rapid Detection of Feline Calicivirus and Feline Herpesvirus by Duplex Nested RT-PCR

Common viral diseases of dogs and cats

Communication of the Results of the Treatment with Probiotics in Two Cats with Chronic Gingivostomatitis

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