Physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic repair material

Abstract

The aim of this study was to evaluate the physicochemical properties, cytotoxicity and bioactivity of a ready-to-use bioceramic material, Bio-C Repair (Angelus), in comparison with White MTA (Angelus) and Biodentine (Septodont). The physicochemical properties of setting time, radiopacity, pH, solubility, dimensional and volumetric changes were evaluated. Biocompatibility and bioactivity were assessed in Saos-2 osteoblast cell cultures by the MTT assay 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), Neutral Red (NR), Alizarin Red (ARS), and cell migration tests. Statistical analysis was performed by ANOVA, Tukey or Bonferroni tests (α = 0.05). Bio-C Repair had the longest setting time (p < 0.05), but radiopacity and solubility were accordance with the ISO 6876/2012 standards, besides linear expansion. Bio-C Repair and MTA had similar volumetric change (p > 0.05); lower than Biodentine (p < 0.05). All the materials evaluated had an alkaline pH. Bio-C Repair was cytocompatible and promoted mineralized nodule deposition in 21 days and cell migration in 3 days. In conclusion, Bio-C Repair had adequate radiopacity above 3mm Al, solubility less than 3%, dimensional expansion, and low volumetric change. In addition, Bio-C Repair promoted an alkaline pH and presented bioactivity and biocompatibility similar to MTA and Biodentine, showing potential for use as a repair material.

Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA

“…These results are in line with SEM micrographs, which reveal that a smaller number of osteoblasts adhered to the Zr MTA samples after 24 h of culture compared with other groups. These results are, however, contrary to those found in other studies [ 12 , 20 , 30 ]. Besides the use of different cell lines and the use of a fresh or set material, previously published studies have evaluated the cellular response in direct contact with pure MTA cements, instead of MTA-based coatings applied on a textured zirconia disc, as in the specific context of this study.…”

“…Mineral trioxide aggregate (MTA) is composed of tricalcium silicate, dicalcium silicate, tricalcium aluminum, calcium oxide, silicon dioxide, aluminum trioxide, and a radiopacifying agent [ 10 , 11 , 12 , 13 ]. Due to its excellent chemical, physical and biological properties, in particular its biocompatibility and osteoconductive potential, it has been used in dentistry for over 20 years [ 10 , 11 , 14 , 15 , 16 , 17 , 18 ].…”

Effect of pH on the solubility and volumetric change of ready-to-use Bio-C Repair bioceramic material

“…BCR has similar cytocompatibility and a higher radiopacity than Biodentine (Septodont, Saint Maur des Fosses, France). 2,3 Bio-C Repair is not cytotoxic to osteoblastic cells, 4 and promoted the deposition of mineralized nodules and cell migration 5 demonstrating its biocompatibility and bioactive potential. 6 BCR showed low volumetric change after immersion in distilled water, with values lower than 3%, after evaluation by micro computed tomography (micro-CT).…”

Biocompatibility and bioactive potential of NeoPUTTY calcium silicate‐based cement: An in vivo study in rats

“…Despite the reduction in cell viability (Klein‐Junior et al., 2021), BC demonstrates to be biocompatible and presents bioactivity in subcutaneous connective tissue (Inada et al., 2023, Queiroz et al., 2023). The prolonged setting time of Bio‐C Repair (Campi et al., 2023) may contribute to cytotoxicity. On the other hand, studies on subcutaneous tissues make it possible to evaluate the in vivo effect of the material.…”