Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

Silva, Léa Assed Bezerra da; Hernández, Patrícia Gatón; Pucinelli, Carolina Maschietto; Paula-Silva, Francisco Wanderley Garcia; Lucisano, Marília Pacifico; Consolaro, Alberto; Sá, Rafaela Cardoso de; Araújo, Lisa Danielly Curcino; Sousa‐Neto, Manoel Damião; Silva, Léa Assed Bezerra

doi:10.1590/0103-6440202203562

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…Contradictorily, whereas studies revealed that Biodentine promoted higher viability and cellular differentiation and mineralization potential (Ali et al, 2019; Rathinam et al, 2021), other studies showed that ProRoot MTA prompted higher osteogenic/odontogenic gene expression and higher mineralization (Kim et al, 2021; Santiago et al, 2021). In vivo , two studies revealed that Biodentine is associated with higher early immunoreactivity (da Fonseca et al, 2019) and gene expression (Silva et al, 2022) of proinflammatory cytokines (IL‐6 and TNF‐α) as compared to MTA. Collectively, these variations in the biological responses may at least be partly due to variable effects of the two materials on the interactions between different cell types, such as cells from inflammatory and stromal lineages.…”

Section: Introductionmentioning

confidence: 99%

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

et al. 2023

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Aim Inflammatory‐regenerative cell interaction is believed to mediate hard tissue formation. This study aimed to investigate the interaction between human inflammatory monocytes with human regenerative fibroblasts after exposure to different calcium silicate materials. Methodology Human monocytes were cultured on three materials, polystyrene (PS), mineral trioxide aggregates (MTA) and biodentine (BD), in the presence or absence of lipopolysaccharide (LPS). Half of the monocyte‐conditioned media (MoCM) of each group was used to analyse inflammatory cytokine secretion, namely TNF‐α, IL‐1β, IL‐1RA and IL‐6. The remaining MoCM was used to culture recipient fibroblasts, measuring the cell number (proliferation) and levels of alkaline phosphatase (differentiation) and lactic acid dehydrogenase (cytotoxicity). Results In absence of LPS, MTA was associated with higher secretion of TNF‐α and lower secretion of IL‐1β, while BD triggered higher secretions of both cytokines when both materials were compared to control (PS). When LPS was added, higher levels of all analysed cytokines were observed in the PS and BD groups, whereas for the MTA group, only TNF‐α and IL‐6 were increased. Fibroblasts responded differently to the MoCM from the different groups, revealing significant increases in proliferation and differentiation capacities, particularly when cultured in CM from monocytes exposed to MTA. The morphological evaluation revealed different patterns of fibroblast shape and spread in the different MoCM groups. Conclusion Calcium silicate materials modulate the monocyte inflammatory response, which subsequently induce differential effects on the recipient fibroblasts. MTA appears to promote the secretion of prodifferentiation signals from the monocytes, which are received by fibroblasts, promoting their proliferation and differentiation. The model represents a promising tool to evaluate the interaction of different cells in response to different materials.

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Section: Introductionmentioning

confidence: 99%

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

et al. 2023

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Mines of cytokine: A treasure trove in pulpal and periapical diseases

Chandwani,

Gedam,

Deshmukh

et al. 2024

Journal of Conservative Dentistry and Endodontics

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Pulpitis is a special disease of dental pulp. It causes localized inflammation, due to various inflammatory mediators such as cytokines and chemokines. These inflammatory mediators are responsible for various reparative and resorptive processes in the dental pulp. The balance between these processes ultimately determines the viability of the tooth. Due to the important properties of various inflammatory markers, the correlation of cytokinin gene expression in various stages of inflammation becomes necessary to focus on. Several studies in the past have focused on the importance of such correlation to help in diagnostic applications. The nature of these inflammatory mediators can help us in diagnostic evaluation. Several attempts have been made to focus on these associations so that it can assist in making clinical decisions effectively. The data available are vast but are the most neglected topic. This review article briefly outlines and summarizes the importance of various inflammatory mediators such as cytokinin and chemokines in various pathways of pulpal and periapical inflammation in explanatory and diagrammatic forms. Knowledge gained about pulpal inflammatory response may aid in understanding the molecular level of inflammatory pulpal and periapical diseases, which shall modify our future diagnostic modalities. Several medicaments are used in the treatment of minimal to advanced dental caries which leads to periapical infections. Thorough understanding of these medicaments can resolve secondary infection and can improve the prognosis of the treated tooth.

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Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

Cited by 2 publications

References 43 publications

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

Mines of cytokine: A treasure trove in pulpal and periapical diseases

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