A New Calcium Silicate-Based Root Canal Dressing: Physical and Chemical Properties, Cytotoxicity and Dentinal Tubule Penetration

Abstract

The aims of this study were to evaluate the physical and chemical properties, cytotoxicity and dentinal tubule penetration of a new calcium silicate-based root canal dressing. For pH and calcium ion release evaluation (1, 24, 72 and 168 h) were used a pH meter and colorimetric spectrophotometer, respectively. Radiopacity evaluation followed the ISO 6876:2012. Cytotoxicity was evaluated by the percentage of cell viability using MTT assay. Illustrative images of dentinal tubule penetration were obtained using confocal laser scanning microscopy (CLSM). Data from pH and calcium ion release were statistically analyzed by two-way analysis of variance and Tukey test. Radiopacity was analyzed using the Student t-test. The statistical tests for cytotoxicity results were the one-way analysis of variance and Tukey test. Both materials showed alkaline pH in all experimental times. The pH values for calcium hydroxide paste were higher than bioceramic paste at 1, 24, and 72 h (p<0.05). The calcium ion release of bioceramic was lower than the calcium hydroxide paste only at 24 h (p<0.05). The bioceramic was more radiopaque than the calcium hydroxide paste (p<0.05). Bioceramic paste presented a dose and time-dependent cytotoxic effect after MTT assay. CLSM images showed absence of tubule penetration for both pastes. The new calcium silicate-based canal dressing presented alkaline pH, high calcium release, and acceptable radiopacity. Bio C Temp showed a dose and time-dependent cytotoxic and absence of dentinal tubule penetration.

Biological parameters, discolouration and radiopacity of calcium silicate‐based materials in a simulated model of partial pulpotomy

“…In the present study, the null hypothesis was rejected, as significant differences were found between the calcium silicate‐based materials in most of the evaluated parameters. Although previous studies have evaluated BD using similar methodologies (Adl et al, 2019; Farrugia et al, 2018; Marconyak et al, 2015; Ochoa‐Rodrıguez et al, 2019; Ochoa‐Rodriguez et al, 2020; Oliveira et al, 2020; Shokouhinejad et al, 2016; Vallés et al, 2015; Yoldas et al, 2016), few studies have used BCT (Guerreiro et al, 2021; Oliveira et al, 2020; Villa et al, 2020) and BCR (Benetti et al, 2019; Ghilotti et al, 2020; López‐García et al, 2019), which are new ready‐to‐use materials. To assess crown discolouration by these materials in the presence of blood components, a model was designed to simulate the clinical situation of partial pulpotomy.…”

“…Other ready‐for‐use silicate‐based materials developed recently are Bio‐C Temp (BCT; Angelus, Londrina, PR, Brazil) and Bio‐C Repair (BCR; Angelus). Both have low cytotoxicity (Ghilotti et al, 2020; Guerreiro et al, 2021; López‐García et al, 2019; Oliveira et al, 2020; Villa et al, 2020), good biocompatibility (Benetti et al, 2019) and biomineralization ability (Benetti et al, 2019; López‐García et al, 2019). BCT was primarily conceived by the manufacturer as an intracanal dressing (Villa et al, 2020) and is also indicated for full pulpotomy (Oliveira et al, 2020).…”

“…Both have low cytotoxicity (Ghilotti et al, 2020; Guerreiro et al, 2021; López‐García et al, 2019; Oliveira et al, 2020; Villa et al, 2020), good biocompatibility (Benetti et al, 2019) and biomineralization ability (Benetti et al, 2019; López‐García et al, 2019). BCT was primarily conceived by the manufacturer as an intracanal dressing (Villa et al, 2020) and is also indicated for full pulpotomy (Oliveira et al, 2020). However, there are no data regarding the use of BCT and BCR for partial pulpotomies in immature traumatized permanent incisors.…”

Análise do pH e da atividade antimicrobiana de um novo medicamento intracanal biocerâmico Bio-C Temp

“…Já o Bio-C Temp apresentou os menores valores de pH em todos os períodos experimentais com diferença significativa com os grupos HC/Soro e HC/PG (P < 0,05). Os materiais a base de silicatos de cálcio, quando hidratados, formam o hidróxido de cálcio que se dissociam rapidamente em íons Ca ++ e OHaumentando o pH do meio (Villa, et al, 2020). No entanto, a baixa solubilidade do HC pode ser responsável pela lenta liberação dos íons OH -, justificando os menores valores do pH do Bio-C Temp.…”

“…O crescente interesse por esses materiais é evidenciado pelo desenvolvimento de novos medicamentos bioativos à base de silicato de cálcio, como o Bio-C Temp (Angelus Indústria de Produtos Odontológicos S/A, Londrina, PR, Brasil). Esse medicamento se apresenta como uma pasta pronta para uso, é biocompatível, possui pH alcalino e boa radiopacidade (Villa, et al, 2020). Além disso, o Bio-C Temp induz a biomineralização e possui atividade antimicrobiana (Guerreiro, et al, 2021).…”