In Vitro Periodontal Ligament Cell Viability in Different Storage Media

Abstract

The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk.

Which is the most recommended medium for the storage and transport of avulsed teeth? A systematic review

“…Overall, 22 studies exclusively recommended natural products as storage media, while two studies recommended them in addition to HBSS and Ringer's lactate . A further three studies also recommend milk and HBSS along with different natural products .…”

Egg White-Milk Mixture as a Novel Biological Storage Media for Avulsed teeth Periodontal Ligament Cells: An In-Vitro Study

“…This finding was in agreement with Krasner., et al who stated that HBSS is effective in preserving periodontal ligament cells of avulsed teeth, renew the degenerated periodontal ligament cells and maintain a superior success rate if an avulsed tooth is soaked in them for 30 minutes [25]. Furthermore, Krasner., et al and Ashkenazi., et al reported that, the pH balanced at 7.2 and osmolality of 320m Osm/kg allowing proper supply of different cell need to prevent starvation and dehydration up to replantation [35,36].…”

“…They were promising concerning the organization of collagen fibres and the number of viable cells. Some suggest that they can be perfect media for storing avulsed teeth but they need additional studies [4,6,[18][19][20][21]. Some considered milk as a gold standard for transporting avulsed teeth.…”

“…While milk came second after 1h and third next to egg white after 3h storage time with insignificant values [24]. However, other studies demonstrated that egg white reported higher viable PDL cells than milk after half hour storage time [25,26]. On the same context Khademi., et al found that teeth stored in egg white for 6 to 10h had a better incidence of repair and lower surface resorption than those stored in milk [20].…”

“…The viable and non-viable cells were counted using digital microscope with magnification 20X with a corresponding special software in area 121 x 129 Mm for each slide in General Pathology Lab. of National Research Centre (Figure 1) [19][20][21]. …”