Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models

Pacheco, Larissa Ferreira; Banzi, Éfani Caroline de Freitas; Rodrigues, Eliana Aparecida; Soares, Luís Eduardo Silva; Pascon, Fernanda Miori; Correr-Sobrinho, Lourenço; Puppin-Rontani, Regina Maria

doi:10.1590/0103-6440201302357

Cited by 27 publications

(36 citation statements)

References 23 publications

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“…The in vitro demineralization models commonly use organic acids, chelators, or bacterial biofilms to mimic carious lesions (34‐36). Although in vitro caries models never reproduce all aspects of the clinical situation, sample acquisition is simple and specimens can be treated in a standardized and reproducible way (37).…”

Section: Discussionmentioning

confidence: 99%

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

Scholz¹,

Hinderberger²,

Widbiller³

et al. 2020

European J Oral Sciences

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Selective caries excavation may support pulp preservation. This in vitro study investigated the influence of selective removal of demineralized dentin on marginal integrity of composite restorations as determined by dye penetration. Dentinal caries‐like lesions were produced in the approximal surfaces of 40 extracted human molars (ethylenediaminetetraacetate, 0.5 M, 96 h). The following test procedures were established: complete excavation, selective excavation, and caries‐free control. Two class II cavities with enamel at the cervical margins were prepared per tooth and demineralization volume was determined by micro‐computed tomography for the purpose of a stratified distribution to receive complete excavation or selective excavation. After complete or selective excavation (30 cavities each), adhesive composite restorations were placed. Cavities without demineralized dentin (20 cavities) served as control. The marginal integrity of restorations was evaluated by dye penetration with and without thermocycling or mechanical loading. Results were analyzed by non‐parametrical statistical tests (Mann‐Whitney U Test) with an α = 0.05 level of significance. Dye penetration did not differ significantly among completely excavated, selectively excavated, or undemineralized teeth, but was increased by thermocycling and mechanical loading in all experimental groups. Selective caries removal did not increase marginal penetration in class II restorations. The presence of remaining demineralized dentin surrounded by sound dentin did not impair marginal integrity of restorations with margins placed in sound enamel.

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Section: Discussionmentioning

confidence: 99%

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

Scholz¹,

Hinderberger²,

Widbiller³

et al. 2020

European J Oral Sciences

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“…In an attempt to simulate and standardize the conditions found in natural cariesaffected dentin, several methods of producing caries-like lesions in vitro have been developed, such as chemical and biological models (Lenzi et al, 2015;Pacheco et al, 2013). The present study submitted the sound dentin specimens to a chemical demineralizing challenge by applying an acid gel containing lactic acid, a by-product of bacterial metabolism (de Carvalho et al, 2008;Pacheco et al, 2013). It was verified that this method was effective in impairing the TBS values of dentin; furthermore, SEM analysis presented a emineralized dentin exhibiting a heterogeneous and porous surface with some lumens of dentinal bliterated (Figure 2 -B1).…”

Section: Discussionmentioning

confidence: 99%

“…2.4.1. Demineralization procedures Tooth slices (N=108) were immersed in 5 mL of 6% carboxymethylcellulose acid gel (Proderma Pharmacy; Piracicaba, SP, Brazil) containing 1 M lactic acid titrated with a concentrated KOH solution at 37ºC, pH 5.0, for 48 h (de Carvalho et al, 2008;Pacheco et al, 2013). Tooth slices were rinsed twice with deionized water in an ultrasonic bath for 15 min and slightly dried with tissue paper (Sacramento et al, 2012).…”

Section: Dentin Demineralization and Surface Pretreatmentmentioning

confidence: 99%

Enhancing bond strength on demineralized dentin by pre-treatment with selective remineralising agents

Martins

Sousa

Castilho

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

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Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue. This study evaluated the effect of 0.2% NaF-(NaF), MI Paste™-(CPP-ACP) and the self-assembling peptide 'P' (Ace-QQRFEWEFEQQ-NH) contained in Curodont™ Repair, have on microtensile bond strength-(µTBS) of two different adhesive systems (Adper™ Single Bond-(SB) or Clearfil™ SE Bond (CSE)) and wettability of demineralized dentin slices after remineralising agents were applied. The highest µTBS were found for the demineralized dentin-(DD) treated with CPP-ACP; both adhesives systems (p < 0.05) did not significantly difference from P treatment associated with SB, and also presented higher values than sound dentin-(SD/SB) (p < 0.01). DD treated with P associated with CSE did not differ from DD/CSE (p > 0.05). The NaF treatment associated with CSE recovered the bond strength values of SD/CSE and associated with CSE demonstrated lower µTBS than other groups, although significantly higher than DD (p < 0.05). P and CPP-ACP increased significantly the wettability of demineralized dentin (p < 0.05); etching acid improved wettability for all groups (p < 0.05), whilst NaF did not affect the wettability of demineralized dentin (p > 0.05). Morphological analysis of the dentin surface and dentin-resin interface revealed unique features of the applied remineralizing agent. The results indicated that self-assembling peptide P associated with SB and CPP-ACP associated with SB or CSE significantly enhanced the bond strength to demineralized dentin (p < 0.05). We conclude that by modifying the dentine surface and restoring conditions found on sound dentin, this can enhance the interfacial bonding.

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“…Altered dentin characteristics can affect the resin/dentin bonding interface, since caries-affected dentin leads to greater variability and a highly irregular composition along the interface (9). Although, clinically caries affected dentin shows mineralized precipitates within the tubules (6), Pacheco et al (10) showed that similar mineral content concerning Ca, CO 3 and PO 4 and organic content like CH bonds, may be found also in vitro when S mutans biofilm provided dentin carious lesions compared with the natural ones.…”

Section: Introductionmentioning

confidence: 99%

Influence of Different Dentin Substrate (Caries-Affected, Caries-Infected, Sound) on Long-Term μTBS

et al. 2017

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The aim of this study was to evaluate the μTBS in different dentin substrates and water-storage periods. Twenty-four dentin blocks obtained from sound third molars were randomly divided into 3 groups: Sound dentin (Sd), Caries-affected dentin (Ca) and Caries-infected dentin (Ci). Dentin blocks from Ca and Ci groups were subjected to artificial caries development (S. mutans biofilm). The softest carious tissue was removed using spherical drills under visual inspection with Caries Detector solution (Ca group). It was considered as Ci (softer and deeply red stained dentin) and Ca (harder and slightly red stained dentin). The Adper Single Bond 2 adhesive system was applied and Z350 composite blocks were built in all groups. Teeth were stored in deionized water for 24 h at 37 ºC and sectioned into beams (1.0 mm 2 section area). The beams from each tooth were randomly divided into three storages periods: 24 h, 6 months or 1 year. Specimens were submitted to µTBS using EZ test machine at a crosshead speed of 1.0 mm/min. Failure mode was examined by SEM. Data from µTBS were submitted to split plot two-way ANOVA and Tukey's HSD tests (α=0.05). The µTBS (MPa) of Sd (41.2) was significantly higher than Ca (32.4) and Ci (27.2), regardless of storage. Ca and Ci after 6 months and 1 year, presented similar µTBS. Mixed and adhesive failures predominated in all groups. The highest µTBS values (48.1±9.1) were found for Sd at 24 h storage. Storage of specimens decreased the µTBS values for all conditions.

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Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models

Cited by 27 publications

References 23 publications

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

Enhancing bond strength on demineralized dentin by pre-treatment with selective remineralising agents

Influence of Different Dentin Substrate (Caries-Affected, Caries-Infected, Sound) on Long-Term μTBS

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