Rat subcutaneous tissue response to MTA Fillapex® and Portland cement

Abstract

The aim of this study was to evaluate the response of rat subcutaneous tissue to MTA Fillapex® (Angelus), an experimental root canal filling material based on Portland cement and propylene glycol (PCPG), and a zinc oxide, eugenol and iodoform (ZOEI) paste. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 7 and 15 days. The specimens were stained with hematoxylin and eosin, and evaluated regarding inflammatory reaction parameters by optical microscopy. The intensity of inflammatory response against the sealers was analyzed by two blinded and previously calibrated examiners for all experimental periods (kappa=0.96). The histological evaluation showed that all materials caused a moderate inflammatory reaction at 7 days, which subsided with time. A greater inflammatory reaction was observed at 7 days in the tubes filled with ZOEI paste. Tubes filled with MTA Fillapex presented some giant cells, macrophages and lymphocytes after 7 days. At 15 days, the presence of fibroblasts and collagen fibers was observed indicating normal tissue healing. The tubes filled with PCPG showed similar results to those observed in MTA Fillapex. At 15 days, the inflammatory reaction was almost absent at the tissue, with several collagen fibers indicating normal tissue healing. Data were analyzed by the nonparametric Kruskal-Wallis test (α=0.05). Statistically significant difference (p<0.05) was found only between PCPG at 15 days and ZOEI at 7 days groups. No significant differences were observed among the other groups/periods (p>0.05). MTA Fillapex and Portland cement added with propylene glycol had greater tissue compatibility than the PCPG paste.

In Situ Assessment of the Setting of Tricalcium Silicate–based Sealers Using a Dentin Pressure Model

“…MTA Fillapex (Angelus, Londrina, Brazil) was among the first sealers based on MTA. Although several publications report physical (9,10), chemical, and biological (11)(12)(13) properties of MTA Fillapex, little is known of its composition and hydration. The material has been shown to be bioactive (14).…”

Biocompatibility of Portland cement combined with different radiopacifying agents

“…Implantation of new endodontic materials into subcutaneous tissues of rats is one of the most suitable methods for determining their local effects and biocompatibility (2,9,10,(22)(23)(24). As well as preliminary tests for materials that are utilized for root-end filling, furcal perforation, and as apical barriers, such materials must be investigated for their biocompatibility characteristics, as toxic components present in them might induce irritation or even degeneration of the surrounding tissues, especially is they are accidentally extruded into the periradicular tissues (4,25).…”

“…This property is of special significance for endodontic materials (3,4) because sealants may cause different reactions in the dental pulp (4)(5)(6)(7). Subcutaneous tissue reaction is one of the in vivo biocompatibility tests that have been used for evaluating several new materials (2,(8)(9)(10).…”

Avaliação comparativa do escoamento e tempo de presa do cimento MTA Fillapex®

“…Para a avaliação do escoamento, Bernardes et al 12 . A alta fluidez do MTA Fillapex ® pode comprometer seu desempenho clínico, pois aumenta a chance de seu extravasamento durante a obturação, o que pode acarretar dor intensa no pós-operatório, uma vez que, em recentes estudos biológicos, o MTA Fillapex ® apresentou maior irritação em culturas de células 14 nas primeiras cinco semanas e moderada reação inflamatória nos primeiros sete dias em tecido conjuntivo 15 .…”