Antibacterial Power of Sodium Hypochlorite Combined with Surfactants and Acetic Acid

Giardino, Luciano; Estrela, Carlos; Mohammadi, Zahed; Palazzi, Flavio

doi:10.1590/0103-6440201300075

Cited by 4 publications

(4 citation statements)

References 23 publications

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“…The authors reported that the number of CFUs after 28 days of irrigation was 50% lower than the specimens treated with solutions containing the detergent in their composition. In agreement with this study, Giardino, Estrela, Mohammadi, and Palazzi () tested the conventional 5.25% NaOCl solution with two other solutions containing a surfactant molecule. The number of CFUs was measured and, the authors observed that after 28 days of sample collection, the solutions containing the surfactant molecule were more efficient than the conventional NaOCl solution.…”

Section: Discussionsupporting

confidence: 61%

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

Vitali

Nomura

Delai

et al. 2019

Microscopy Res & Technique

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In this study, we evaluated the effectiveness of 1 and 5.25% NaOCl solutions, with or without surfactant, in disinfecting gutta‐percha cones. Surface changes of the gutta‐percha cones after disinfection were also assessed under scanning electron microscopy (SEM). One‐hundred and eight gutta‐percha cones previously contaminated with Enterococcus faecalis were left in contact for 30 s or 1 min with the following solutions (n = 12): 1% NaOCl; 1% NaOCl + 2% cetrimide; 5.25% NaOCl; 5.25% NaOCl + 2% cetrimide. Positive control group was composed by the remaining contaminated cones (n = 12), and the negative control group by noncontaminated cones (n = 12). The cones were kept in Eppendorf tubes containing Trypticase Soy Broth at 37°C for 24 hr, and then, the presence of turbidity was analyzed. Ten additional cones (n = 2) were observed under SEM to evaluate surface changes after 1 min of disinfection. The data analysis (Kruskal–Wallis test, α = 5%) demonstrated that regardless the presence of surfactant, the NaOCl solutions used were effective in the disinfection when cones were immersed for 1 min. However, at the 30‐s period, only the 5.25% NaOCl solution was effective. SEM analysis demonstrated that NaOCl solutions promoted the formation of sodium chloride crystals on cones surface, less visible when the 1% NaOCl associated to surfactant was used. The addition of surfactant did not affect the disinfection capacity of NaOCl solutions. However, the surfactant has apparently inhibited the sodium chloride crystals deposition on the cones surface when associated to 1% NaOCl solution.

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Section: Discussionsupporting

confidence: 61%

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

Vitali

Nomura

Delai

et al. 2019

Microscopy Res & Technique

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“…Palazzi et al (7) showed that the surface tension of NaOCl solutions with added detergents (Hypoclean and Chlor-XTRA) were lower than NaOCl, agreeing with the findings of the present study. An ex vivo study compared the antibacterial power of 1% NaOCl with 1% acetic acid, 5.25% NaOCl and two NaOCl modified with surfactants in bovine root dentin at different days (22). According to the findings, all the NaOCl solutions showed small number of CFU over 28 days.…”

Section: Influence Of Temperature On Naoclmentioning

confidence: 99%

Influence of Temperature on the Antibacterial Activity of Sodium Hypochlorite

et al. 2016

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The aim of this study was to compare the antimicrobial activity of 5.25% NaOCl, Hypoclean and Chlor-Xtra at 20 °C and 45 °C in bovine root dentin. One-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with Enterococcus faecalis. The specimens were divided into the following groups: 1. 5.25% NaOCl 20 °C; 2. Hypoclean 20 °C; 3. Chlor-Xtra 20 °C; 4. 5.25% % NaOCl 45 °C; 5. Hypoclean 45 °C; 6. Chlor-Xtra 45 °C; 7. positive control; 8. negative control. Dentin chips were collected with round burs into Brain Heart Infusion (BHI) broth. After culturing, the number of colony-forming units (CFU) was counted. Statistical analyses were performed using descriptive statistics (mean, standard deviation, median), Shapiro-Wilk test, ANOVA and Tukey test. Significance level was set at p<0.05. In all experimental groups, CFU was minimum after treatment (day 0) and the obtained results were significantly different from each other at any period (p<0.05). After treatment, the Hypoclean and Chlor-Xtra showed the lowest numbers of CFU at 20 °C and 45 °C, whereas 5.25% NaOCl showed the highest number of CFU at both temperatures. In each group, the number of CFUs increased significantly with time (p<0.05). The antibacterial activity of Hypoclean and Chlor-Xtra at 45 °C were significantly greater than other tested solutions.

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“…Endodontic infections are controlled by means of instrumentation, irrigation and intracanal medication. However, these procedures do not completely eliminate the microorganisms in root canal systems (Giardino, Estrela, Mohammadi, & Palazzi, ). Therefore, root canal filling must prevent periapical tissue fluids from reaching these microorganisms, and should also act as a barrier preventing the microorganisms from attaining the periapical tissues (Donadio et al, ).…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility, physical properties, and antibiofilm activity of endodontic sealers with amoxicillin

et al. 2017

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The aim was to evaluate the cytocompatibility of the AH Plus and Sealapex associated with amoxicillin (AA) in a quantity of 10% by total weight of the sealers, the flow and setting time of these sealers with 10%-0.25% AA and antibiofilm activity of these sealers associated with AA in a concentration that does not alter their physical properties (1%). Cytocompatibility was assessed by MTT, neutral red and cytoskeletal fluorescence assays. Setting time and flow was evaluated using the specifications of ISO 6876/2012. For the antibiofilm evaluation, materials were placed in direct contact with E. faecalis biofilm induced on dentin blocks. The incorporation of AA into the sealers did not diminish the viability of the fibroblasts and did not cause structural changes in the cytoskeletal. The flow of AH Plus + AA at 1.0; 0.5 and 0.25%, and of Sealapex + AA at 5.5; 2.5; 1.0; 0.5 and 0.25% were within the specifications of the ISO 6876. AH Plus, and AH Plus + AA at 1.0; 0.50 and 0.25% presented a setting time of 730, 439, 455, and 474 min., respectively. Sealapex - pure or associated with AA did not set completely. The addition of 1% AA to the sealers did not increase their antibiofilm activity. It was concluded that the incorporation of 10% AA caused no toxic effects on fibroblasts, but harmed the physical properties. The addition of AA in concentration that does not affect the physical properties of the sealers did not increase their antibiofilm activity.

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Antibacterial Power of Sodium Hypochlorite Combined with Surfactants and Acetic Acid

Cited by 4 publications

References 23 publications

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

Disinfection and surface changes of gutta‐percha cones after immersion in sodium hypochlorite solution containing surfactant

Influence of Temperature on the Antibacterial Activity of Sodium Hypochlorite

Cytocompatibility, physical properties, and antibiofilm activity of endodontic sealers with amoxicillin

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